LOCUS       sequence-479842-        6207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IFNGR1-LgBiT Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: IFNGR1-LgBiT Vector
FEATURES             Location/Qualifiers
     source          1..6207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3150..3168)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    3205..3326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3216..3235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3270..3289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3571..3928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3571..3762
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3594..3614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3779..3914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3943..3990
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4022..4816
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4076..4095)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4686..4705
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4880..4928
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5167..5755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5247..5266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5871..6154
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct ctcctctttc
     1081 tcctacccct tgtcatgcag ggtgtgagca gggctgagat gggcaccgcg gatctggggc
     1141 cgtcctcagt gcctacacca actaatgtta caattgaatc ctataacatg aaccctatcg
     1201 tatattggga gtaccagatc atgccacagg tccctgtttt taccgtagag gtaaagaact
     1261 atggtgttaa gaattcagaa tggattgatg cctgcatcaa tatttctcat cattattgta
     1321 atatttctga tcatgttggt gatccatcaa attctctttg ggtcagagtt aaagccaggg
     1381 ttggacaaaa agaatctgcc tatgcaaagt cagaagaatt tgctgtatgc cgagatggaa
     1441 aaattggacc acctaaactg gatatcagaa aggaggagaa gcaaatcatg attgacatat
     1501 ttcacccttc agtttttgta aatggagacg agcaggaagt cgattatgat cccgaaacta
     1561 cctgttacat tagggtgtac aatgtgtatg tgagaatgaa cggaagtgag atccagtata
     1621 aaatactcac gcagaaggaa gatgattgtg acgagattca gtgccagtta gcgattccag
     1681 tatcctcact gaattctcag tactgtgttt cagcagaagg agtcttacat gtgtggggtg
     1741 ttacaactga aaagtcaaaa gaagtttgta ttaccatttt caatagcagt ataaaaggtt
     1801 ctctttggat tccagttgtt gctgctttac tactctttct agtgcttagc ctggtattca
     1861 tctgttttta tattaagaaa attaatccat tgaaggaaaa aagcataata ttacccaagt
     1921 ccttgatctc tgtggtaaga agtgctactt tagagacaaa acctgaatca aaatatgtat
     1981 cactcatcac gtcataccag ccattttcct tagaaaagga ggtggtctgt gaagagccgt
     2041 tgtctccagc aacagttcca ggcatgcata ccgaagacaa tccaggaaaa gtggaacata
     2101 cagaagaact ttctagtata acagaagtgg tgactactga agaaaatatt cctgacgtgg
     2161 tcccgggcag ccatctgact ccaatagaga gagagagttc ttcaccttta agtagtaacc
     2221 agtctgaacc tggcagcatc gctttaaact cgtatcactc cagaaattgt tctgagagtg
     2281 atcactccag aaatggtttt gatactgatt ccagctgtct ggaatcacat agctccttat
     2341 ctgactcaga atttccccca aataataaag gtgaaataaa aacagaagga caagagctca
     2401 taaccgtaat aaaagccccc acctcctttg gttatgataa accacatgtg ctagtggatc
     2461 tacttgtgga tgatagcggt aaagagtcct tgattggtta tagaccaaca gaagattcca
     2521 aagaattttc agtttctcaa ggcagttcag gtggtggcgg gagcggaggt ggaggctcga
     2581 gcggtgtctt cacactcgaa gatttcgttg gggactggga acagacagcc gcctacaacc
     2641 tggaccaagt ccttgaacag ggaggtgtgt ccagtttgct gcagaatctc gccgtgtccg
     2701 taactccgat ccaaaggatt gtccggagcg gtgaaaatgc cctgaagatc gacatccatg
     2761 tcatcatccc gtatgaaggt ctgagcgccg accaaatggc ccagatcgaa gaggtgttta
     2821 aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgccctat ggcacactgg
     2881 taatcgacgg ggttacgccg aacatgctga actatttcgg acggccgtat gaaggcatcg
     2941 ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc aacaaaatta
     3001 tcgacgagcg cctgatcacc cccgacggct ccatgctgtt ccgagtaacc atcaacagct
     3061 aaggccgcga ctctagagtc gacctgcagg catgcaagct gatccggctg ctaacaaagc
     3121 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     3181 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3241 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3301 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3361 ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3421 tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     3481 atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac
     3541 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt
     3601 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3661 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3721 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3781 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3841 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3901 cttttttgga ggcctaggct tttgcaaaaa gcttaattaa ctgttgacaa ttaatcatcg
     3961 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccca ggaggcagat
     4021 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4081 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4141 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4201 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4261 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4321 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4381 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4441 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4501 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4561 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4621 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4681 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4741 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4801 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4861 ctgccatcac gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     4921 tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc ttgtccagat
     4981 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctaccc
     5041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5581 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga
     5761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac
     5881 aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc
     5941 agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt
     6001 taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     6061 tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt
     6121 aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga
     6181 tgcgtccggc gtagaggatc gagatct
//
