LOCUS       sequence-48014-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2314
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             67..132
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
       61 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      121 gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcaccg cggggtacct
      181 atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag
      241 gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg
      301 catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa
      361 gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag
      421 tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct
      481 ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg
      541 gtagaggcag tgcacgcctg gcgcaatgct ctcaccgggg cccccttgaa cctgacccca
      601 gaccaggtag tcgcaatcgc gtcaaacgga gggggaaagc aagccctgga aaccgtgcaa
      661 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt
      721 gcatcccacg acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc
      781 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgaataa caatggaggg
      841 aaacaagcat tggagactgt ccaacggctc c
//
