LOCUS       sequence-481543-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of human ENDOG downstream of SP6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pTwist Amp High Copy ENDOG-HA
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..43
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     35..52
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     36..52
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        60..78
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     60..77
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             992..1018
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1068..1087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1069..1087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1106..1122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1106..1122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgga
       61 tttaggtgac actatagaat acgaattcga gctcggtacc atgagggcac tgcgagctgg
      121 cctgacactg gcctcaggcg ctggcctggg ggccgtcgtg gagggctgga ggcggcgaag
      181 agaagatgcc agagctgctc ccgggctcct cggccgattg ccggtgctgc ctgtggcggc
      241 tgccgccgaa ctccctccag tgcctggcgg gccgagaggt cctggcgaac tcgccaaata
      301 cggtctcccc ggtctcgctc agctgaagag tagagagtct tatgttctct gctacgaccc
      361 ccggaccagg ggagcattgt gggttgtgga acaactgaga cccgaacgcc tcagggggga
      421 tggagacagg agagagtgcg atttccggga ggatgattct gtgcacgcat atcacagggc
      481 gacaaacgca gactatagag gaagcggatt tgatcgtggg catctggcag ctgcagccaa
      541 ccatcggtgg tcccagaaag ctatggacga taccttctat ttgtccaatg tggcacccca
      601 ggtcccacac cttaaccaga atgcttggaa taacctcgag aagtactccc gttctctgac
      661 gcgttcctac caaaacgtgt acgtatgcac aggtcctctc ttcctcccca ggaccgaggc
      721 tgacggcaag agctacgtca aataccaggt tataggcaaa aaccacgtgg ccgttcccac
      781 ccacttcttt aaagttctga tattagaagc cgctggtgga cagatcgagc tgaggaccta
      841 tgttatgcca aatgctcctg tcgacgaagc aataccgctt gaacggtttc tggtccccat
      901 tgagtcaatt gaacgtgcga gcgggctgct ctttgtgcct aacattttgg cacgggcagg
      961 gtccctcaag gccatcaccg cgggaagtaa atacccatac gatgttccag attacgctta
     1021 atagtgagga tcctctagag tcgacctgca ggcatgcaag cttgtctccc tatagtgagt
     1081 cgtattagag cttggcgtaa tcatggtcat agctgtttcc tgtgtga
//
