LOCUS       sequence-482035-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express DCAF1-HaloTag Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DCAF1-HaloTag Vector
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..26)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      191..326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             371..769
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    793..841
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(991..1008)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1125..1670)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1205..1224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1887..2681)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1998..2017)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2608..2627
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2713..2760)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2770..2803
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2918..3039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3009..3028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3126..4010)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             complement(4026..4046)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             7366..7377
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8601..8620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8602..8620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8662..8681)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          8664..8796
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(8932..9135)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8961..8981)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9136..9439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    9672..9753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        join(9832..9849,1..340)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(9832..9849,1..174)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
       61 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
      361 tagcgccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
      421 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
      481 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
      541 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
      601 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
      661 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
      721 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaaa ctctagccat
      781 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
      841 gacgattccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
      901 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
      961 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     1021 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     1081 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     1141 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     1201 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     1261 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     1321 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     1381 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     1441 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     1501 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     1561 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     1621 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     1681 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac
     1741 ctgaagtcag ccccatacga tataagttgt aattctcatg tttgacatgt tctttcctgc
     1801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
     1861 ggcgtcgctt ggtcggtcat ttcgaatcag aagaactcgt caagaaggcg atagaaggcg
     1921 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     1981 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     2041 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     2101 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     2161 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     2221 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     2281 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     2341 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     2401 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     2461 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     2521 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     2581 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     2641 gcggccggag aacctgcgtg caatccatct tgttcaatca tgatctgcct cctgggttta
     2701 gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac
     2761 agtggatcca taacttcgta taatgtatgc tatacgaagt tatattagaa ttcttaatta
     2821 agattttacc acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct
     2881 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc
     3061 ttgctcagcg gtggcagcag ccaaatcagc ttgcatgcct gcaggtcgac tctagaattc
     3121 tattaaccgg aaatctccag agtagacagc cagcgcgcga tctcgctgcc gatcaggtcc
     3181 gggttgtctt cttgcagcag attcagaccc gggccgatgt ccacagcctt gcagttaggc
     3241 aggcttttgg ccaggcgagc ggcttcggcc ggtgggatca gaacgcctgg ggtgccccag
     3301 aacagcagct tcgggacagg ggactggtgc agccagtcca tgtattcttc gaccagcgcg
     3361 acgatgttcg ctggctcacc ggcgattggc agctcgtttg ggaagcgcca cagtggctcg
     3421 cggtcaacag gattcaggaa cggctcgcgg taatggtcca tctcgacttc agtcagcggg
     3481 cggacgacac ccatcggcag cgtaccctcg ataaaaacgt tctgatcgat gatcagcttg
     3541 cggccgacgt cggtggtgcg gaaggcctgg aaggtctcgc gggcaaattc tggccattcg
     3601 tcccaggtcg ggatagggcg gatgaactcc ataaatgcaa tacctttgac gcgctctgga
     3661 ttgcgcttgg cccagtggaa acccagagcg gagccccagt cgtgaatgac caggacgacc
     3721 tcttccagac ccagggcttc gatgaaggca tccatgaagc ggacgtggtc gtcgaagaaa
     3781 taacccaggt ctggtttgtc ggatttgccc ataccgatca ggtctggagc aatgcagcga
     3841 tgggtcggtg caacatgcgg gatgatgttg cgccacacgt aggaggaggt cgggttaccg
     3901 tgcaggaaca gcacaggggt gccatcgcgc ggaccaacat cgacgtagtg catgcgctcg
     3961 cccaggactt ccacataatg ggggtcgaat ggaaagccag taccgatttc ggatccatcg
     4021 ttatcgctct gaaagtacag atcctcagtg gttggctcga gagaaacctc attcagagat
     4081 aagatgatgt catcttccaa atcagagttg tcagagctgt cagtgtcccc caggatcagt
     4141 tccacctcct catctgcatc agagtcttca tcctccccgt cctctccctc ctctagaagg
     4201 tttgctagct cctcatcaga gggagagaag tcattgtccc catcttcccc tgcgttctca
     4261 ttattgtcgt cctcctccaa ctccgcctcc agcaactggt cagtgtcaag ctcatctaaa
     4321 tcatcggtgt catcatcatc ttcatcatca tcttcttcct cctgttcttc ctcttcctgg
     4381 tcctcctctt catcctcatc ctctgccaga cgctgcctgc ccacttcata cagcctgcat
     4441 actgtgtcca tgttcagggc atccatgctg ccttgattct caatgacagc aagatagcag
     4501 tctttggtgt ctgtacacag gtcaaagatg ttccgtttca catcaatggt tgctataggt
     4561 ttgtagtcag ttgcattaaa tgttcggaag gatgacccaa aggggctttt catcctctct
     4621 tccattaagt catcttcatc atctgcctgc aacatagctc catacatcac tgttcccgtg
     4681 tgattgaaca ccacgcgaca ctgatccaga gcgggaacag tatgcaaaag atgaaaagtt
     4741 cgaaggtccc aaatctcagt attaatgatc acctccagtc catttggatg gaaaacacca
     4801 ctgatgttca tattgaactt gtcaaacttg tggatggcct gtgcagagcg gacatcccag
     4861 aggacgccat catttaagac aagatcatct gtaggattaa aggtggcaca gttcctcttg
     4921 tagttgttgg caagatctgg gttaaacaga gtcaacagct tgttgccagt ctgaatatca
     4981 taaatgtggg caatgtctcc ttttgtgccg atgacccgat cctgggagtg cttactgaac
     5041 tcaacatagt gatcttctgt gaaggaatgc ttcatatcaa atactgactt cattccccaa
     5101 agtgcagaca aaggctggct ccaagtagca gatgtcagca gcaaggaccc atccctggaa
     5161 ggttcaagat gtgtgatggc tgagttgtga cagttatagc tggcctcctc ctgtccacta
     5221 aacacattat agagcttcag ctgccctgtg caggtgccaa gcatcaggaa ccgctcccgt
     5281 gctgagaatg cacagcaggt gaagccactc tcatcttcat tggcttcccg gaacactgaa
     5341 ataggacgga atctgctaaa gataaggtgc ctatcaaagc atccgccatc cacccctcca
     5401 tactttggaa atgatgccct gcggtttagc cttgacgtaa agtttattgg cgcttgccgc
     5461 ctctgttttg gctcaggaca ttggtgagga gtaaagaggg agaaaggtgg gcaggtggca
     5521 actggattct tgcagcgagc atgttgttct ctaagatact ctgtgattat actgtccagc
     5581 gtaggtgggg aaggaagatg tctgtccagc tgttttttta tggctgggct ttggctgtag
     5641 gcaccatggt ccgacttctg ccgcaacact ctgattttcc tgccattgca gggtgatggc
     5701 ctctctctga taaaactgat tctaccaatc aaaggggagt tgcctgcata agatgggccg
     5761 ggcagagcta gcggaccctg ggggggccgt ggctgaggat gagcagtagg ggcagaaggc
     5821 gcagaggcac ccacagcagc atggctgccc agacgagttg caatgccatt agcgatacga
     5881 ggggttcggg gtagagagac aggagaagca gcagcagtga ctggggtaaa ggcagaagaa
     5941 tgggaggcag cagtcatggg caggtcagcc tcttttgtca gcacggttgc tgtttctcca
     6001 agccctttag aaataagatg gtttcgtatc aacaaaagca gctctttctc agggaaggag
     6061 atccttgact gggcaacaac atctgctttc tgcagtcgtg ctagggaaac atcagtgcca
     6121 atgagaagtg gttttcctga cacccgttca atgagttcag cagcatactt gcagaacttg
     6181 acatggtcac tgcgcttgtc ctgcagcaca ggctccttca tcagctgctg gatctggcag
     6241 ctgctgaaaa ggggcagttt actgatgatc tgccggacag tgctactgcg agacaggccc
     6301 actagggctt tgcaggccag ggcccggatt tggtctgcat ctgtgatggg catcttaatg
     6361 gacagtaagg acaggagcac cttgatgccg ttgttggact gaaccacatt ccacatcttg
     6421 gccagggtgt gctcactgct tttagggttc tgaggcagct ttctccgagg agtaccagag
     6481 ataaatttac caatactgga tattcggtta tctgggccac acacacaatt gatgataatc
     6541 tgaagtgctg acttctgaat ttcagcatca tggatgaaga actcaccctc agccactccc
     6601 aaaataatgc tgatacctac agtagagact gtagatccag cctcatccaa cacgtccact
     6661 gattctgcca actggagctg gatttttggc accacagtaa gaatagccag gacatccaaa
     6721 gcaaagcgca cagtgtcatt ccttgcataa taggtcttcc aattgcaggc aatagaaata
     6781 agctgcaaca agagttgcac acaagaaagt ttgaggaaaa cttcagctgg ttcccaatat
     6841 agctgcgctg ggccatattc tatcaaaaat tccatcattt ccacaatctg ttcatgagta
     6901 tatgagcatg ccttgtacgg gggttgtggg tggacaagaa tgccaccctc agtcctctga
     6961 agtgactgct tcacttgttc caatttaatg gccaggtgag cctcaaagta tttgcgcaag
     7021 gccatgcagg tatgtttccc agtttggcgg ctagcaaata tttcatcatc actcagaagt
     7081 gcaccctgat cttccaaatt tagaatctcc aaagtactga tcaagttcac cagacgacga
     7141 agaccatcat agcggtcaaa gagctccaag acggcccgaa atgagaagca aattgaaaaa
     7201 aacatggtag catggcagca tcctgaagca tgagaacact ccattaacca cagggtatag
     7261 ttcaccacat cagacagaac attgtgggga tgcatgcaaa ctctttccat ggcatcctga
     7321 ttgtaggata ggtaatacaa acacatagac acaccggttg cagccataga aggacgaggt
     7381 atttccagta atttctgtac tccaccatgc gcaacaaatt ctgtggcaaa tttgttatgg
     7441 agcaggagag atgctaggtg ctttagtgcc tcaaatgtaa gcaggacatc attagtttgc
     7501 ttcaggtcaa tatagaacat catcagctcc cgtgatccaa gttgcatgaa tatgggaagt
     7561 agctcctgat attctcctag aggggtcaaa tattggagaa tgagtcgctg ctcgatagca
     7621 ggagtcatag gataaagggt ataattggtg ccaatcaccc agggagacat ttctgaccaa
     7681 ctgctattag acagctcaac aaacatgcga tctggatcag aagatgagaa acccaacttt
     7741 tgcttggctt tcctaaagtt ctctctgtca ccctgttttg ctgacttgtt tttctttaat
     7801 cctccatcct caggtttggt tgttgagttc actctgctac tagtcttgtg gcctgaatca
     7861 agatgaaagg agatctccat gtctccagaa gcttcctctt ggtctccatc cactacatct
     7921 acagccatgt caccatagtc catatccaca gcctcctcat ccagaggcaa aaggggttca
     7981 gaagagagct tccgtggact gggacgcttg ttttcctgcc gcaaagccac ttcctgtagc
     8041 tgtagctccc tcagtcttcg aagcactatt gccaccagct gtgaattttc atctctatag
     8101 ttggcagcaa tgtcttgatt ttccatagca cctcctaaca gtccagtaga atatgtcctc
     8161 aatggttgat cggcctctcg ggcccatttg aaaagattct cgacaattcc ctccttttct
     8221 tgaaagacga cagcagtttc cagccctggc atgatgtcta ataggagtct gcaagctgca
     8281 gtgtttaaag ggggctctcg gcttgtcatc acatatgcat tcaccagtgc attcatgaaa
     8341 tcatcattct tgaagagtat tctcagcaag tggcccagca tacactctgg atcagctcga
     8401 ccaggatgtc gatcatcaaa tgggtctgga tcccctttac gatactcttc agtttctttt
     8461 tcaatcaatt gagacatcct ggtaaggata ggtaccatgt cctgcccact gccatgttcc
     8521 ttttcccact gctccagcag ggtagtgagc tcagctttgg agtccacatg taccactact
     8581 gtagtcatgg cgatcgctag ccctatagtg agtcgtatta atactctagc cttaagagct
     8641 gtaattgaac tgggagtgga cacctgtgga gagaaaggca aagtggatgt cagtaagacc
     8701 aataggtgcc tatcagaaac gcaagagtct tctctgtctc gacaagccca gtttctattg
     8761 gtctccttaa acctgtcttg taaccttgat acttacctgc ccagtgcctc acgaccaact
     8821 tctgcagctt aagttcgaga ctgttgtgtc agaagcactg actgcgttag caatttaact
     8881 gtgataaact accgcaataa agcttctagt gatctgacgg ttcactaaac cagctctgct
     8941 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaacgggg cggggttatt
     9001 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     9061 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tggtgtactg
     9121 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     9181 aaagtcccgt aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     9241 gtcaataggg ggcggacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     9301 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     9361 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     9421 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     9481 tgattactat taataactag tggcgcgcca taatcctagg ctctcgtacg aagtcagctg
     9541 ataacgtcga cgtattgcgg ccgcggatca taatcagcca taccacattt gtagaggttt
     9601 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     9661 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9721 caaatttcac aaataaagca tttttttcac tgccgacgtc ttaattaacg cgtcttgcgc
     9781 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     9841 aaagaacca
//
