LOCUS       sequence-482046-       21370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a CRISPR/dCas9-based fusion protein designed for targeted 
            DNA demethylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21370)
  TITLE     Versatile molecular tools enabling customizable DNA methylation in 
            Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TET1cd-dCas9-IBM1 (D30)
FEATURES             Location/Qualifiers
     source          1..21370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4103..4123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4124..4150,4151..4153,4154..6640,6641..6643,
                     6644..8227)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4124..8227
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8243..8263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8267..8287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8336..9031
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     terminator      12196..12448
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12477..12493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12477..12493)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12490..12512)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12501..12517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12525..12531,12532..12549,12550..12555))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12570..12591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12782..13458
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     13395..13415
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             13525..14550
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    14591..14765
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14843..14867
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15292..16086
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16173..16761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16662..16681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16915..16932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    16947..17087
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(17013..17032)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17173..17195
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      17431..17625
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(17691..18764)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(19193..19822)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    21123..21147
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     21335..21357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     21349..21366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     21350..21366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagac ccggggatat attactcaac ttttgattcg
       61 ctatttgctg tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca
      121 ttgctattac aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca
      181 taaccatttt ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac
      241 cagctccgtt tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc
      301 tttgattgat cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa
      361 acattgcatt cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag
      421 ctttctacat gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa
      481 gcacgaacac cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg
      541 ttgcgatatt gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt
      601 aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca
      661 ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag
      721 ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt
      781 cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa
      841 actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg
      901 gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata
      961 ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct
     1021 ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta
     1081 gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt
     1141 ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga
     1201 tcactgactt ggttctaacc aaaaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccatgct gcccacctgc agctgtcttg atcgagttat
     1741 acaaaaagac aaaggcccat attatacaca ccttggggca ggaccaagtg ttgctgctgt
     1801 cagggaaatc atggagaata ggtatggtca aaaaggaaac gcaataagga tagaaatagt
     1861 agtgtacacc ggtaaagaag ggaaaagctc tcatgggtgt ccaattgcta agtgggtttt
     1921 aagaagaagc agtgatgaag aaaaagttct ttgtttggtc cggcagcgta caggccacca
     1981 ctgtccaact gctgtgatgg tggtgctcat catggtgtgg gatggcatcc ctcttccaat
     2041 ggccgaccgg ctatacacag agctcacaga gaatctaaag tcatacaatg ggcaccctac
     2101 cgacagaaga tgcaccctca atgaaaatcg tacctgtaca tgtcaaggaa ttgatccaga
     2161 gacttgtgga gcttcattct cttttggctg ttcatggagt atgtacttta atggctgtaa
     2221 gtttggtaga agcccaagcc ccagaagatt tagaattgat ccaagctctc ccttacatga
     2281 aaaaaacctt gaagataact tacagagttt ggctacacga ttagctccaa tttataagca
     2341 gtatgctcca gtagcttacc aaaatcaggt ggaatatgaa aatgttgccc gagaatgtcg
     2401 gcttggcagc aaggaaggtc gacccttctc tggggtcact gcttgcctgg acttctgtgc
     2461 tcatccccac agggacattc acaacatgaa taatggaagc actgtggttt gtaccttaac
     2521 tcgagaagat aaccgctctt tgggtgttat tcctcaagat gagcagctcc atgtgctacc
     2581 tctttataag ctttcagaca cagatgagtt tggctccaag gaaggaatgg aagccaagat
     2641 caaatctggg gccatcgagg tcctggcacc ccgccgcaaa aaaagaacgt gtttcactca
     2701 gcctgttccc cgttctggaa agaagagggc tgcgatgatg acagaggttc ttgcacataa
     2761 gataagggca gtggaaaaga aacctattcc ccgaatcaag cggaagaata actcaacaac
     2821 aacaaacaac agtaagcctt cgtcactgcc aaccttaggg agtaacactg aaaccgtgca
     2881 acctgaagta aaaagtgaaa ccgaacccca ttttatctta aaaagttcag acaacactaa
     2941 aacttattcg ctgatgccat ccgctcctca cccagtgaaa gaggcatctc caggcttctc
     3001 ctggtccccg aagactgctt cagccacacc agctccactg aagaatgacg caacagcctc
     3061 atgcgggttt tcagaaagaa gcagcactcc ccactgtacg atgccttcgg gaagactcag
     3121 tggtgccaat gctgcagctg ctgatggccc tggcatttca cagcttggcg aagtggctcc
     3181 tctccccacc ctgtctgctc ctgtgatgga gcccctcatt aattctgagc cttccactgg
     3241 tgtgactgag ccgctaacgc ctcatcagcc aaaccaccag ccctccttcc tcacctctcc
     3301 tcaagacctt gcctcttctc caatggaaga agatgagcag cattctgaag cagatgagcc
     3361 tccatcagac gaacccctat ctgatgaccc cctgtcacct gctgaggaga aattgcccca
     3421 cattgatgag tattggtcag acagtgagca catctttttg gatgcaaata ttggtggggt
     3481 ggccatcgca cctgctcacg gctcggtttt gattgagtgt gcccggcgag agctgcacgc
     3541 taccactcct gttgagcacc ccaaccgtaa tcatccaacc cgcctctccc ttgtctttta
     3601 ccagcacaaa aacctaaata agccccaaca tggttttgaa ctaaacaaga ttaagtttga
     3661 ggctaaagaa gctaagaata agaaaatgaa ggcctcagag caaaaagacc aggcagctaa
     3721 tgaaggtcca gaacagtcct ctgaagtaaa tgaattgaac caaattcctt ctcataaagc
     3781 attaacatta acccatgaca atgttgtcac cgtgtcccct tatgctctca cacacgttgc
     3841 ggggccctat aaccattggg tcggagggcc gagctctggc gcacccccac caagtggagg
     3901 gtctcctgcc gggtccccaa catctactga agaaggcacc agcgaatccg caacgcccga
     3961 gtcaggccct ggtacatcca cagaaccatc tgaaggtagt gcgcctggtt ccccagctgg
     4021 aagccctact tccaccgaag aaggcacgtc aaccgaacca agtgaaggat ctgcccctgg
     4081 gaccagcact gaaccatctg agccaaaaaa gaagagaaag gtaatggaca agaagtacag
     4141 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     4201 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     4261 cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc ggctgaagag
     4321 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     4381 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     4441 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     4501 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     4561 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     4621 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     4681 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     4741 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     4801 cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct
     4861 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     4921 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     4981 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     5041 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     5101 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     5161 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca tcgatggcgg
     5221 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     5281 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     5341 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     5401 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     5461 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     5521 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     5581 cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     5641 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg agctgaccaa
     5701 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     5761 agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     5821 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     5881 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     5941 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     6001 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     6061 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     6121 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     6181 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     6241 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     6301 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     6361 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     6421 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     6481 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     6541 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     6601 ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt
     6661 tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga accggggcaa
     6721 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcgccagct
     6781 gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg ccgagagagg
     6841 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     6901 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     6961 cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     7021 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     7081 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     7141 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     7201 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     7261 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     7321 cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg tgcggaaagt
     7381 gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     7441 caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa agaaggactg
     7501 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     7561 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     7621 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     7681 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     7741 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     7801 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     7861 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca aacactacct
     7921 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     7981 tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca gagagcaggc
     8041 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     8101 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     8161 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     8221 aggcgacggt ggcggaggct cgccaaaaaa gaagagaaag gtagacccaa agaaaaaacg
     8281 aaaagtaagc ggaagtgaga ccccaggtac atccgaatca gcaacgcctg aaagcagcga
     8341 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     8401 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     8461 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     8521 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     8581 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     8641 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     8701 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     8761 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     8821 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     8881 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     8941 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     9001 cctccctagc aaactggggc acaagcttaa tggcggtggc ggagggatgg atgctaagtc
     9061 actaactgcc tggtccatgg attctgtgga ggaagaaggt gttgttaggg ttgaagaaga
     9121 gaacggacgc ggtggtctcc gtaggcatcg gagggtttct acgaagctgg cgaattacgt
     9181 agatcctccg actgacgacg aagaagatgg aggacccaag cgaaagggca aaaggggtgg
     9241 aaatagggct ccgaaaaaga caccgaagaa agacgaggag atgcaaaaga acgaaattga
     9301 tgaagcaaac cgagtcactg gtctggtgaa ggaaaaaaga gccgcgacta agattctgaa
     9361 tcgcaaagac tccattattg aagtaggaga agcttctgga agcatgccca aggaagtaaa
     9421 gggaattagg attgggaaaa ggaagggaga aatcgatggt gagattccaa ctaagccggg
     9481 caagaagcca aagactacag tagacccgag aatcattggt tacagaccgg acaatatgtg
     9541 ccatcaatgt caaaagagcg ataggattgt tgaacggtgt cagacctgca atagtaagcg
     9601 ttattgtcat ccatgcttgg acacttggta tccccttata gcaaaagaag atgttgccaa
     9661 gaaatgcatg ttctgctcta gtacttgcaa ttgcagagca tgcttgcgtc tagatactaa
     9721 attgaaaggg ataaattcga acctcatagt cagcgaggaa gaaaaggtcc aagcctctaa
     9781 gtttattctg cagagccttc tcccacatct gaaaggaata aatgatgaac aagttgctga
     9841 gaaggaagtt gaggccaaaa tatacggact gaagtttgaa gaggtgaggc cccaagacgc
     9901 taaagctttc cctgatgaaa gactatactg tgatatctgc aagacttcta tctatgatct
     9961 ccataggaat tgcaagtcct gtagttttga tatctgcctt agctgttgcc ttgagatccg
    10021 caatggaaag gctctggcat gtaaggagga tgtgtcttgg aattatatta accgaggttt
    10081 agagtatgaa catggacaag aaggaaaagt gattgaaaag ccggcgaata agctagatga
    10141 taagttgaaa gataagctgg atggtaagcc ggatgataag ccgaagggta agccaaaggg
    10201 taggccaaag ggtaagccgg atgataagcc gaagggtaaa ctgaagggta agcaggatga
    10261 taaaccggat gataagccag atgagaagcc ggttaataca gaccatatga agtacccttc
    10321 tctgtggaaa gcaaatgaag ctgggatcat tacttgttgt tgtggtgctg gggagttagt
    10381 gctgaaacga ctacttccgg atggttggat atctgagttg gtcaacagag ttgaaaaaac
    10441 tgctgaagct ggcgagcttt tgaatttacc tgaaacggtt ttggagcgat gcccttgttc
    10501 taactctgac agacatattg acatagacag ctgtaatttg ttaaaagctg cttgtcgaga
    10561 aggttcagaa gacaattatc tttactctcc aagtgtatgg gatgttcaac aagatgattt
    10621 gaagcatttt cagcaccatt gggtaaaagg cgagcctgtg attgtgagaa atgtgcttga
    10681 agctacatct ggtttaagct gggaaccaat ggtaatgcat cgtgcctgcc gccagataag
    10741 ccatgtccag catggatcac ttaaggatgt tgttgctgtt gattgtttgg acttctgtga
    10801 ggtaaaagtt aatcttcatg aattttttac tggatacacg gatggccgat atgatcgaat
    10861 gggttggcca ctagttctga aactgaaaga ttggcctcca gctaaagtct tcaaagataa
    10921 cttgccacgt catgctgagg agttcttatg tagtttgcct ttgaagcatt acactcatcc
    10981 ggttaatgga cctttgaatc tcgctgtcaa gcttccccaa aattgcttga agccagacat
    11041 gggaccaaag acgtacgttg cttctggatt tgctcaagaa ttaggccgtg gagattctgt
    11101 tactaagctc cactgcgaca tgtctgatgc ggtgaatatt ttgacgcaca tatctgaagt
    11161 gcccaatatg caacctggaa ttggaaatct gaaaaagaag catgccgaac aagatctcaa
    11221 ggagctgtat agctcagtag ctaacaagga ggaaatgatg gagattcttg aaaattccag
    11281 acaacaagtc caaaatgttg aaactgatga tggagctctt tgggacattt tccgtagaga
    11341 agatattcct aaattagaaa gttacattga gaaacatcac aaggagttca gacatctcta
    11401 ctgctgtcct gtgtctcagg ttgttcatcc tatacatgac cagaacttct atctgacgcg
    11461 gtatcatata atgaagctga aagaagaata tggcattgaa ccttggacct tcaatcagaa
    11521 gcttggtgat gctgttttga tacctgtagg ttgccctcat caagttagaa atttgaagtc
    11581 ctgcaataag gtagcgcttg acttcgtctc acctgaaaat gtcagcgagt gtttacgctt
    11641 gacaaaacag tatcgtctac ttccaccaaa tcattttgca aaagaagaca agttaggggt
    11701 taagaagatg atagtccatg cagttgataa agctctcaga gacttaagtg gagagaagtc
    11761 tccagaacct gaagagaaga aacaaaatat gagagggccg aagaaggggg cagccaaggc
    11821 agttgccaag gctctcaaag acttatctcc aagtgaaaag aagtcttcgg aagcagctga
    11881 agaggagata tcaaatggga tagtcaatgc aatcgataag ggtctcaaag acttacctcc
    11941 aagtgaagag aagtcttccg aagccaaagt ggagatatca aatgggatag tcagtgcaat
    12001 ggataaggat ctcgaacaca tatcttcaag cgaaaagaag tctacggaag aggaaggggt
    12061 gaaaagacca aatattgtga ggacatatga gcggagaaaa aagctgggaa gtgaagtaac
    12121 caacgcatac attgacagat tagaaatgga gaagatgtaa ggatcctgat tgatcgatag
    12181 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
    12241 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
    12301 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
    12361 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
    12421 cgcgcggtgt catctatgtt actagatcgg ggtaccgagc tcgaattcgt aatcatgtca
    12481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    12541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    12601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    12661 caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgccaac atggtggagc
    12721 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
    12781 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
    12841 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
    12901 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
    12961 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
    13021 tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
    13081 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
    13141 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
    13201 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct
    13261 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
    13321 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
    13381 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
    13441 ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt
    13501 cgcagatccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
    13561 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
    13621 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
    13681 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
    13741 ccgattccgg aagtgcttga cattggggag tttagcgaga gcctgaccta ttgcatctcc
    13801 cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttcta
    13861 caaccggtcg cggaggctat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
    13921 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
    13981 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
    14041 tccgtcgcgc aggctctcga tgatctgatg ctttgggccg aggactgccc cgaagtccgg
    14101 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
    14161 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    14221 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    14281 catccggagc ttgcaggatc gccacgactc cgggcgtata tgctccgcat tggtcttgac
    14341 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    14401 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
    14461 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
    14521 cccagcactc gtccgagggc aaagaaatag agtagatgcc gaccgggatc tgtcgatcga
    14581 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
    14641 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
    14701 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
    14761 agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
    14821 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
    14881 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
    14941 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
    15001 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
    15061 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat
    15121 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
    15181 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
    15241 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
    15301 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
    15361 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
    15421 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    15481 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    15541 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    15601 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    15661 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    15721 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
    15781 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    15841 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    15901 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    15961 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
    16021 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    16081 ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    16141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    16201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    16261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    16321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    16381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    16441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    16501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    16561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    16621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    16681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    16741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    16801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    16861 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    16921 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
    16981 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    17041 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
    17101 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    17161 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    17221 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
    17281 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
    17341 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
    17401 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
    17461 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
    17521 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
    17581 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
    17641 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
    17701 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
    17761 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
    17821 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
    17881 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
    17941 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
    18001 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
    18061 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
    18121 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
    18181 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
    18241 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
    18301 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
    18361 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
    18421 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
    18481 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    18541 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    18601 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    18661 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    18721 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    18781 tgggggttcc agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac
    18841 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    18901 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    18961 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    19021 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    19081 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    19141 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    19201 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    19261 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    19321 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    19381 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    19441 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    19501 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    19561 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    19621 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    19681 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    19741 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    19801 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    19861 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    19921 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    19981 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    20041 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    20101 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    20161 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    20221 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    20281 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    20341 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    20401 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    20461 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    20521 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    20581 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    20641 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    20701 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    20761 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    20821 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    20881 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    20941 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    21001 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    21061 gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt
    21121 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
    21181 acaatctgat ccaagctcaa gctgctctag cattcgccat tcaggctgcg caactgttgg
    21241 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    21301 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    21361 gccagtgcca
//
