LOCUS       sequence-48242-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM
  TITLE     Truncated and full-length thioredoxin-1 have opposing activating and
            inhibitory properties for human complement with relevance to 
            endothelial surfaces.
  JOURNAL   J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19.
  PUBMED    22430737
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2012-04-15"; volume: "188(8)"; pages: "4103-12. 
            Epub 2012 Mar 19"
COMMENT     Sequence Label: pET16b-Trx CC32/35SS
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..64
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             77..88
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(460..478)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      474..521
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(696..701,702..718,719..724))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(751..769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggccatcat catcatcatc atcatcatca
       61 tcacagcagc ggccatatcg aaggtcgtca tatggtgaag cagatcgaga gcaagactgc
      121 ttttcaggaa gccttggacg ctgcaggtga taaacttgta gtagttgact tctcagccac
      181 gtggagtggg cctagcaaaa tgatcaagcc tttctttcat tccctctctg aaaagtattc
      241 caacgtgata ttccttgaag tagatgtgga tgactgtcag gatgttgctt cagagtgtga
      301 agtcaaatgc atgccaacat tccagttttt taagaaggga caaaaggtgg gtgaattttc
      361 tggagccaat aaggaaaagc ttgaagccac cattaatgaa ttagtctaag gatccggctg
      421 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      481 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      541 ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      601 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      661 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
      721 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
//
