LOCUS       sequence-48269-e        2200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pSB4A5 JQ929581
FEATURES             Location/Qualifiers
     source          1..2200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    749..798
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     promoter        1057..1091
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     protein_bind    1234..1281
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1323..2033
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGIAHGMDELY"
     terminator      2059..2130
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2146..2173
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2180..2200
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agttatcaac gatgtcctaa tttcgacgga aggtcacaat
       61 aacgggctac ggctacttca tgttgttcaa cgtaggtttc tttatcagct tctttaatac
      121 gttctaagcg acgatcaaca tagtatactc caggcatttt aaggttttta gcgggttttt
      181 tggaacgata ggttgttttc aggttgcaga ttaaatgacc gcctccaact aatttcagtg
      241 ccatgtctgc gcgtccttct aagccaccgt ccgcaggata taaggtttct gttgatgctt
      301 cccaaccaag agtttttttt tgcattacgg gtccgttact cgggaaattt acgccacgga
      361 ttttaacatt gtaaatcaga cagccatctt gtaaactcgt atcttgagtt gctgtaagta
      421 caccgccatc ttcataagtt gttacacgtt cccaggtaaa gccttcagga aaggactgtt
      481 taaaaaagtc aggaatgcct tgggtgtgat tgataaaagt tttagacccg tacataaagc
      541 tcgttgcgag aatgtcaaac gcaaagggca ggggtccacc ttcaactgct ttgatgcgca
      601 ttgtttgtgt gccttcatat ggtttacctt caccttcgga tgtgcatttg aaatgatgat
      661 tgttgacagt accttccata tataatttca tgtgcatatt ttctttaatt aattctgaca
      721 ttaatatata cctcttaatt tttactagtc ggccggcttg tcgacgacgg cggtctccgt
      781 cgtcaggatc atccgggccc ctcctgaggt gccacccgtg cggcacgttg aagccgatta
      841 gtacaataga ttttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc
      901 caaaagcaaa aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag
      961 ccggttattc aaccccaaag gtctactacc gtgctaacgc tcgctaaggc ccggtccgga
     1021 agcacgccgt cgctctcgcg cctatacagt tgtcacttga cagctagctc agtcctaggt
     1081 ataatgctag cagatggtta gtgtgcgtat ctgatacttt tatgtctgtc ctacaactcc
     1141 aagcgtacag ttcaggccaa caccctagct ttgagaaagt cgcgtcagta aatgtcgttt
     1201 ccctaaagct tacaatttac ctcctcgcta ttgggtttgt accgtacacc actgagaccg
     1261 cggtggttga ccagacaaac cacgatacta gagaaaagat cttttaagaa ggagatatac
     1321 atatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg
     1381 gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg
     1441 gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc
     1501 tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc
     1561 agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct
     1621 ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg
     1681 taaaccgcat tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata
     1741 agctggaata caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg
     1801 gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg
     1861 atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact
     1921 atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc
     1981 tgctggagtt cgtaaccgca gcgggcatcg cgcatggtat ggatgaactg tacagatgat
     2041 gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2101 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2161 gggcctttct gcgtttatat actagtagcg gccgctgcag
//
