LOCUS       sequence-48270-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pSB4A5 JQ929582
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        complement(940..974)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             1323..2036
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2060..2131
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2147..2174
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2181..2201
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agttatcaac gatgtcctaa tttcgacgga aggtcacaat
       61 aacgggctac ggctacttca tgttgttcaa cgtaggtttc tttatcagct tctttaatac
      121 gttctaagcg acgatcaaca tagtatactc caggcatttt aaggttttta gcgggttttt
      181 tggaacgata ggttgttttc aggttgcaga ttaaatgacc gcctccaact aatttcagtg
      241 ccatgtctgc gcgtccttct aagccaccgt ccgcaggata taaggtttct gttgatgctt
      301 cccaaccaag agtttttttt tgcattacgg gtccgttact cgggaaattt acgccacgga
      361 ttttaacatt gtaaatcaga cagccatctt gtaaactcgt atcttgagtt gctgtaagta
      421 caccgccatc ttcataagtt gttacacgtt cccaggtaaa gccttcagga aaggactgtt
      481 taaaaaagtc aggaatgcct tgggtgtgat tgataaaagt tttagacccg tacataaagc
      541 tcgttgcgag aatgtcaaac gcaaagggca ggggtccacc ttcaactgct ttgatgcgca
      601 ttgtttgtgt gccttcatat ggtttacctt caccttcgga tgtgcatttg aaatgatgat
      661 tgttgacagt accttccata tataatttca tgtgcatatt ttctttaatt aattctgaca
      721 ttaatatata cctcttaatt tttactagtc ggccggcttg tcgacgacgg cggtctcagt
      781 ggtgtacggt acaaacccaa tagcgaggag gtaaattgta agctttaggg aaacgacatt
      841 tactgacgcg actttctcaa agctagggtg ttggcctgaa ctgtacgctt ggagttgtag
      901 gacagacata aaagtatcag atacgcacac taaccatctg ctagcattat acctaggact
      961 gagctagctg tcaagtgaca actgtatagg cgcgagagcg acggcgtgct tccggaccgg
     1021 gccttagcga gcgttagcac ggtagtagac ctttggggtt gaataaccgg cttatcggtc
     1081 agtttcacct gatttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga
     1141 aagatgaatg actgtccacg acgctatacc caaaagaaaa tctattgtac taatcggctt
     1201 caacgtgccg cacgggtggc acctcaggag gggcccggat gatcctgacg acggagaccg
     1261 cggtggttga ccagacaaac cacgatacta gagaaaagat cttttaagaa ggagatatac
     1321 atatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg
     1381 gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg
     1441 gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc
     1501 tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc
     1561 agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct
     1621 ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg
     1681 taaaccgcat tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata
     1741 agctggaata caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg
     1801 gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg
     1861 atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact
     1921 atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc
     1981 tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatgat
     2041 gatatctaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2101 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2161 tgggcctttc tgcgtttata tactagtagc ggccgctgca g
//
