LOCUS       sequence-482806-       21989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Melezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1,
            and pBAD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21989)
  AUTHORS   Al'Abri IS, Haller DJ, Li Z, Crook N
  TITLE     Inducible directed evolution of complex phenotypes in bacteria.
  JOURNAL   Nucleic Acids Res. 2022 Jun 10;50(10):e58. doi: 10.1093/nar/gkac094.
  PUBMED    35150576
REFERENCE   2  (bases 1 to 21989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2022-06-10"; volume: "50(10):e58. doi"; pages: "
            10.1093/nar/gkac094"
COMMENT     Sequence Label: ISA861: Melezitose pathway
FEATURES             Location/Qualifiers
     source          1..21989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             941..1600
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1007..1026)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     RBS             4059..4067
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(17195..18073)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        18100..18384
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     18307..18326
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      18636..18707
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      18723..18750
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    complement(21885..21909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagcc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacactct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgcg gatctttgat
      841 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      901 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      961 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1021 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1081 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1141 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1201 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1261 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1321 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1381 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1441 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1501 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1561 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     1621 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1681 tcggttgttc catatcgcct attcccgctc tggcccactg gtcgcgccgg gctcgaccac
     1741 cacaggcacg gttgtatgag gatcgtccaa ggtcttcccc tcaaccaaat ccagtgcctt
     1801 gcgcgcggca atctgtccga tttcgcgcgg gggagttggc accatggtgc ttaatccaat
     1861 ggattccgcc acgtcggacc catcaaagcc aaagaccgaa acgtcttcgg ggattcgtac
     1921 tccgactgcc tgcaactcat gtaccacgcg caatgcgtag tagtcagacc acacgcagat
     1981 agcggtcggc cgctcaggct gcgcgagcat ggtggcgatg acctgtttgg ccgagcgcgg
     2041 tgattccgag tcgatgaagt ggatgccgca gtcggtgagg ccaagtgaga ctatctcatc
     2101 tttgtatccg cgtgcgcgtg catcgaatcc ccattccttg ccggtcagcg gatcggtgcg
     2161 ccccacatag cagaaacgac gatgcccgag ggccgcaaga taacgcactg ccatggcttc
     2221 ggctgcctcg tcatcaatgg ccacggaggc ttggcagcag ctgatatacg gcgagttcac
     2281 cgagacgatg ggcataccca tgctggccaa cgtgtcggct tcctcatcgg tggcggaaaa
     2341 tccggatacg atgagggcgt cggcattgcg attggccggt aaccgtgtaa agaattccgc
     2401 acgctcctcg acgctgccgg ctcggtatac ggtcaagtca tagcgtgcgc ggctcagttc
     2461 gtcatacagc ccgtcgaata attcgcccat gaaccagccg gccagccgtt cgcgagccag
     2521 catggcaatg cgattggtgc gtccgcttgc cagcgccgcc gccgagcgag acgcggtgta
     2581 attcagctct ttggccactt tcagcacctt atcccgcgta gctgctgcta cgtgatcctt
     2641 acctcgcaaa gcgcgggatg cggtaaaagt tgagactccg gccgtgagag ctacgtcgtt
     2701 gatggtcgcg cgtttcatgg tgaatgggtg gccgccttgt ggctgttacc cggtcgtcct
     2761 ttctctggct ggtatctgtt gtttcgctat tgcaataatg atatgtgcat catcaatgcg
     2821 gacgttgtcg atgtttaatc tcgaggtgct gggcctgtgg tgccgcgcaa catgagcgtc
     2881 gtgggaacaa cgatgcaact ttctgtcaga atttcgcctc gtgataatcg gattgcggca
     2941 tctgccgcta atgctccgta ggaccgaggg tcgtgccgaa tggttgatat attgagcgct
     3001 tgtgccattg gccagtcatc gaatccgatg acagatatgt cctcaggtac gcggaatccg
     3061 tatgagtgaa gcgcatggat tagaggaatg gcattctcgt cgctgatgca gcatacggcg
     3121 gttgggcgag gggagagtgc gagcaggcta ctgagtgcct ctgctccggc aaattcgttg
     3181 tcctgtactg cgatgatgta tccgtcgggc aagttgagtc cctcagccgc catgccaaga
     3241 cggaaccctt ctatacgttg gtcagcttcc catatgaatc cgttactggc ttgctgcttg
     3301 cagataaagg cgatattgcg atgtcccagt cctgcgagct gatgaacggt ttgtcgcatt
     3361 gctttaattt cgtctacgcc gatggaaatc atatcttgct gttccgtaag cccgatggtg
     3421 ttgacaccga tgactggcat accgagttgt tctaaggttc gcctctctgt ctcgctgatg
     3481 tcgaaggagg agatgatgac cgcgtctgcg ttgcgcttca ctggcattga ggaaaagaaa
     3541 tcgcgtcttt gatcggcgtt tgcaagtcgg tatgatagca agtcatatcc ttgggatgcg
     3601 agcacggaat atacgccttc ggctagttgt gaactgaacc agccagccag tgagttacct
     3661 acggtaagtg ctatacgatt tgtctttccc gttcgcagca tggaggctgt ttttgatgct
     3721 gaaaacccca actttgcaca tgcatcaaat accttttgcc tagtagtttc cgatatgcgg
     3781 tcacgtccgt tgattgcacg agatacggtc gccgcagaga ctccagcctc cttggctact
     3841 tcatagattg ttggttcgct catgggatct cctacattgc gtaatcgatt gtattgtatc
     3901 tggattttga agttaaagtc gccattggtg tggtgcaaga ttgacgggtt ccgcaaaaag
     3961 atgtacgatg tgcgtaatcg atatcgcaaa tgtattttat tgctgccgaa aaaaatggtg
     4021 acaataaaga gaaatatcga ttgcatatga tgagggtata aggaggtatc cgatgaatag
     4081 gattatgcgt aacagcggcg cggtgcttgc tgcgatattt atcgctggaa gcctgactgc
     4141 atgtggtcct ggctcggcaa gtagctcacg aagtgataca aatcctactg atgtcagcac
     4201 tgatttaggt aataaaaaat atgagctgac tttgtgggat ggagcgggtc tgaagactgt
     4261 agatgaggcg ctgattgccg ggttcgaagc gaaataccca aacatcacca tcaaaggaca
     4321 atatgatccg gataatgtct ctggacaaaa tggcccgcgt gtgatttcag caaaagatgc
     4381 gccggatatc gcccgcgtca ccgacatgaa ttcagcggtt cgtggcaacc atttggtgtc
     4441 tttagaggca tatgtggatg cgtacggctg ggatgttccc gattcgcaga ctgaattgta
     4501 tcgagtggat tcaaatggca agctcggcag cggtgatttg tacgcattgc cgaactccta
     4561 ttcggtgact ggcatttatt tcaataccaa gcttgccgag cagctaggta ttgatgcagc
     4621 tcctacctct gtcgaggagt ttgaggcgga tatgcagaca gccaaggatg ccggcatact
     4681 gccgatgatg acatatgcca aggatggcgg tacctctttt gtctttcagg cgttaatggc
     4741 taataacagt tctgcggaag atgtgcagaa ttggattttg cagaaatctg gtaccttcga
     4801 caatcaggcg gcgcaggacg ctgcgtccac cttgcaggat tggaataaca agggatatat
     4861 gcctgaaggt gttaacgcag tggatgcctc tactgctctg agcaggttct gcaatggtga
     4921 aggactattt ttcccgagcg gtaattggaa tctggatact gtggccaaag cgctgggtga
     4981 tgatgttcag ttctttgctt tcccgggtgc caccagtgaa gatgagccga acgttgctgc
     5041 taatgcaggt gcattttatg gtattccggt gaatgctaag aaccgtgatg ctgctgcggc
     5101 gttcctcgat tatacgcaaa gtgccgaagc gcaacagatt attgttgaca attctggcta
     5161 tttgccaaaa tcatctactt tggatctcaa ggcgaatagc gcattgcagc agagtatgtt
     5221 tgatgcctat gccgacgtgc tgacgagcgg tcatactagc gatttcatta ataatgctac
     5281 tgcgggaatg cagtcaagtg ggctaatccc taacttccag ttactgctgg ataacagcat
     5341 cacgcctcag gaattcacaa agaatgtgca ggcgcaatat gacaaagaag cgaagcggta
     5401 gaaacagctt caacttatat gccgctttcg ctagcgacgc ttgatttgag gagagtcaac
     5461 ctatgatgat ggcaggaccg tacgcgggaa ctttatcgga tttgccgact gtccgcgacg
     5521 aagtaacatg ttcgattaat gcggagaacc caacgggagc caagggtttt ggttgccgtg
     5581 aaactggtcc gttggggccg tcgcgcaagg gaaagccgtg tttgaatgac attgctcccg
     5641 ggcagtctgt gacattggca gatattgaag gccccggttg tattcggcac atgtggttta
     5701 ccgttacgga tcgcaccagt gacgctgatc gatacgttct gcgcgatgcg gtgcttgcca
     5761 tctattggga tgatgaaaag cgaccttctg tggaatgtcc cctaggagac ttcttctgtt
     5821 gcggctttgg acaaagttgc gatgtattct cagagcctat tgtggtggcg cctacacgag
     5881 gcatgaattg ttatttcacg atgccgtttc gtaaacgggc gcgtatcgta gtgcgcaatg
     5941 accacgctca tgctattccg gctttgtttt atcagattga ttatgcattg ggtaatgcgt
     6001 attcctcccg tgaaatggct tatttccatg cgcaatggcg cagacagcca atcactactt
     6061 taggcgagga ctatgtaatt ctcgatggag tcagcggacg gggccattac gtcggcacat
     6121 atcttgctat ttctacgttg caacgatatt ggtggggtga gggagagatt aaattttttc
     6181 tagatggcga taacgattat cccacagtgt gtagtaccgg taccgaagat tattttggcg
     6241 gtgcatggag cttcgctgag cagcgagatg gtcatacgcg cgagcgtact ttttgtgccc
     6301 catatgtagg atatccattt tattctgtgc gcgatggggc tatagcaaac caatatcatg
     6361 acgattattg tccacctatg cgtggcttgt accgatggca tattcctgat cccatcaggt
     6421 ttgatagtga gcttagagtc acgcttcaac aaattggttc aggatatgaa ggtgcatttg
     6481 agcgtcagga tgatgtgtct agcgtggcgt actggtatca aattgagcca cattcaattt
     6541 ttcccgagct gcccgagcct tcgaaacgtc atcctaggtg aatgaataag cgcttaggta
     6601 ttttaaggga ccgatgtcgc aatttcgcga gaaggaagtg cttgcctatg gggaacgtca
     6661 agccttcctc atttcttcgg gaatcggcca tttgtttgcg tgttgcggat ttgacgaacc
     6721 ggttcgacat ctgttatcct catgcataag cgcttagcaa atacgctaag ccgagatagc
     6781 caaggaagac atagccggga tcgcccggaa ggaagaaagg aataatcaat gaggatcgaa
     6841 cgaaccgcca aaaccgttat tgccggtctg atgtctgtgg catgcctggc atccgtcgcc
     6901 gcttgcggcc cgggctcgtc cagcaacaaa gccaacaccg aaaccgaagc ggtgagcact
     6961 gatcttggcg ataccaagta tgagctcaag ctgtgggacg gcgctggcct gaagaccttt
     7021 gatgaccagc tgatcgaagc cttccagaag aagtacccga acatcaccat caaggccacc
     7081 tacgatccgg acaacacctc ccagcagaat ggcccgcgca tcatctccgc cgccgacacc
     7141 ccggacattg cccgtatcac cgacatcaac tccgctgtgc gtggcaacca tgtcgtcaat
     7201 cttgacgctt acgccgatgc ttacggttgg aagctgccgg attcccagac tcaggtctac
     7261 cgcgtgggct ccgacggtaa gatcggttcc ggcagcctct acgcagtgcc ggacggtgtc
     7321 tcgatgaccg gcctgtactg gaacaagaaa gtcgccaagg aactcggcat caccgaggct
     7381 ccggccactg tcgaagagct cgaggccgat atgaagaagg cctccgacgc tggcaagctc
     7441 gccatgatga tgccggccaa ggaaggcggc acttcctaca tctaccaggc attgctcacc
     7501 aactatgagg gtcgcgatac cgtgcaggat tggattatcc agaaggatgg cgccaccttc
     7561 aacaccgacg gtgccgtgaa ggctgcacag aagatcaagg actggcagga tgccggttac
     7621 ttctcctccg acgcgctcgc ccttgacggt tccactgcac tgagccgctt ctgcaacggc
     7681 gaagccctgt tcttcccctc cggcagctgg tactccgcct ccatcaacga tgcacttggc
     7741 gatgacgccg gctggatcgc cttccccggc gaaaaggccg attccggttc tgccgcagcc
     7801 aacgccgtga ccgctttcgg tatcccggcc aacgccaaga acaagaacgc tgcagccgcc
     7861 ttcctcgact tcctccagtc cgacgaagcc cgccagatcg cagttgacaa cggctacccg
     7921 ccggtcggcg aaggcgagac tccgagcacc gataaccagc ttctcggcca ggtccttacc
     7981 gcctacgaag gtctggtgaa gaccggtaac accaccgact acatcaacaa cgccaccgca
     8041 ggtatgcagg ccagcgccat tatccccggc ttccagtccc tgattgacgg cacgatgacc
     8101 cccaaggcat tcgtcgaatc cattcaggct cagtacgaga aggaagccaa gtaacccttg
     8161 cacttaccgg gcggagtgtt cttccctttt tcccgctccg cctggttcct tcccatcgcg
     8221 cgcacgacgt tgtgtgcgat gttcgattcc cagttcacac caaaccagca aagcgtggtg
     8281 acaatcatgg attcccataa ggcagccaag gcggtcaagc ccaagcgcaa tatgtccctc
     8341 ggtctggtca gcctgctttt cctcgctccg gcgcttgtgt tctacatcgc cttcgagttg
     8401 tggccgatca ttcagaccat ctggtactcg ttctacgagt ggaacggtat cgatgctagc
     8461 accttcatcg gtgtcgaaaa ctacatcacc gtgttcaccg accccgatct gtacggatcg
     8521 attctgcact ccttctacct gatcatcttc ttctccatca tcccgattgt gctcggcctg
     8581 atcattgccg tgctcatcaa agacatgaag tccaaggtcg gtcgttcctt cgcgcaggtt
     8641 tgcctgttcc tgccgcgcgt tatcccaggc gctgcagccg gtgtggcctg gacctggatg
     8701 ctggccgaca agggcaccgc caatcagctg ctgcgcgcag taggcctcgg cgacttcgcc
     8761 cacgtgtggc tcggcgacca atccacctcc ctgaacgcgg taggcgtgat tggtttctgg
     8821 ttgcagctcg gcttctgtgt ggtgctcctg ctctcgggca tcggcggcat cgaccagtcc
     8881 ctgtacgagg cagcaagtct tgacggtgcc ggctggtggc gtcagctctt ctccatcacg
     8941 ttgcctggtc tgcgcggcca aatcggcgta tgcctgacca tgaccatcat ctccgcgctc
     9001 gccagcttcg acgtggtgta catgtccact cagggcggcc ccggcacctc caccatggtg
     9061 cccggcgtgc aggtctaccg cttggccttc acccagcaga gcgttggctt ggcctccgcg
     9121 ctcgccgtca ccctgatgat tctggtgctg ctggtcgtcg gacctctgca gcgtctggtc
     9181 aatggaaagg agcagtgacc atgcagcaat ccaaagccgt tcgcatcacc ggcacggcga
     9241 tgctcgctct gaccgtcctc ttctgcgtac tgccgttctt gagcatgctc tccgcggcat
     9301 tgcagccgca aggttccatg cccgaaggta tccagttcac tgcccacccg cactgggaga
     9361 acttcatcga cgcctggaac atggcaaaca tcaccccgct gctgctcaac agctgcattc
     9421 tggtgattgt ggtcgtgccc tgcgtggtga tcttctgctc gctcggtggt tatgccttcg
     9481 ctcagctcaa ggtgccgtgc aagggcctga tctacgtgct gttcctcgtc ggtctgacca
     9541 ttccgttcga aaccttggtc actccgctgt actacgagat cagcggcatg ggcttgctga
     9601 atacccgtct tgccctgatc ctgccgctca tcggcctgaa tattccgttc ggtattgtgt
     9661 ggatgcgctc ctgcttcgag cagatgccgc gtgatctgat cgaagccgca tccatcgatg
     9721 gtgcaggcca tctgcgcacg ttccgttcca ttcagctgcc gttggcactg ccagccatct
     9781 cctcgctcgg tattctgacc ttcctggcca cctggaacca gttcctgttg gctgtggtgt
     9841 tggagaacga cccgaacaag cgcacgatgg caggtgccct acagtcgttc gttggccagt
     9901 atcagaccga cgtggtgctg ctcaacgcgg gcgcactgct catcatggca ccgacgatga
     9961 tcctgttcat cttcctccag cgctacttca tccgcgccat gcttgccggc tcggtcaagg
    10021 gctgatccga aggcaagagg atgtatggta tggccgccgg aacagtgttt cgtgcggcca
    10081 tactggtaaa accatacgta ccgagcaatc ggacagggga acgatatgac tttcaccaac
    10141 aatgactggt ggcgcgacgc cgtcatctac cagatttacc cccgctcatt cagcgatgcc
    10201 aatggagacg gcaacggtga tctccaaggc gtgatcgatc gtctcgacta tctgcaggcg
    10261 ctcggggtgg acgcgctctg gctttctcca ttctatccat cgccgctggc ggatggaggt
    10321 tatgatgttg ccgactattg cgatgtggat ccccgccttg gcacactcga tcagttcgac
    10381 gaattagtgg ccaaagcgca cgaacgcggt atcggcatca tcgtggacat cgtgccgaac
    10441 cacacgtccg atcagcaccg ttggttccaa gaggccctgg ctcaaggtcc ggaatcggaa
    10501 acggcacaac ggtatgtgtt ccggcagggc aagggggagc acggcgaatt gccgcctacc
    10561 aactggctga gtaacttcgg cggttccgca tgggagtcgt gtggagacgg ctggtactac
    10621 ctgcatctgt tcgccaagga gcagccggac ctcaattggg acaatcccga ggtgcggcat
    10681 gagttcctgc gtgtactcac attctggtgc gatcgcggcg tggacggttt ccgcatcgat
    10741 gtctcgcacg gtctggccaa agacctgcgt gagccgctgc gcgaccgtat cgacccgaca
    10801 ctgatgtctc cacaggctac cgatggttcc gatccgctct gggaccgcga cgccgtgcat
    10861 gatatctacc gtgaatggcg cgacctgttc aatcagtaca tgccagccaa gtatgcggtc
    10921 ggcgaatcgt ggtcgccatt caccacccgt attttccaat acgccaagcc tgatgaactt
    10981 ggtgccgtat ttgacttctc catgtcaaag gccgcctgga atcgcgaaga ataccgcacc
    11041 acgattgagc gcacgcatcg ctatgctctc gctgccgaaa ccgcccccac ctgggtgctc
    11101 ggcaaccacg atgtgccacg tatcgcctcg cgtctcggac tgccgagcgg tgcaaacatc
    11161 gagcagtggg tgacgtccaa cggcacgaat ccgccgattg accccgcgct cgccgctcgt
    11221 cgtgcccgtg cagcagcgct ggtgatgctc ggcctgccag gtaccgcctt tgtgtatcag
    11281 ggtgaagagc ttggtttgcc tgaggacttt gatttgaccg aagatgaaat tcaggatccc
    11341 aattgggaac gttccggtca ttatttcaag gggcgcgacg gttgccgcgt accgctgccg
    11401 tggcagtcag acggcccggc gttcggcttc aatgccaccg gtgctttgtg gctgccccag
    11461 ccggcatggt ttgcgcagta tgccgctaat cgtcagattg gtgacggggc ttccgtcctg
    11521 cacctgtatc gtgctgccat cgcattgcgc aaacagtggg tggacaacgg cacggacttc
    11581 cagttctcgt ggcttaccaa cgaagagtgt ccggcgccgc tgcttggttg gcgcttgccg
    11641 tccggcatgg ttgtgatggc gaacttctct gacagtcaac cagtcgcctt gccgcagccg
    11701 atgaccgtgc tacttgtgtc tgatgcgact gtgcaggcga acactcaggt cgagagcgtt
    11761 cctccagctg ctaccgtctg ggccttgcca gcataatctc attgaagaac accaaggagt
    11821 tacgccaatg attgccgaaa cctgtccaat aaccgttcgc acggtaacgg cccatcttga
    11881 taccggtcgt gccgtctatg gcgaacaggc ttcggcaatc tgtgaactgc cggtagaagc
    11941 cgtcacgatc gttcccgaat catgcgaaga cgagtgcacg tccagtgcgg gtatccaggg
    12001 tgatgctcag gtgcgactct ggcgtatcga tgtgaacgcg catgcacccc agcccaagga
    12061 ccgcgccgct gcttttcagg aacaacaagc gtttgagccc caccatgcga ttgacgtgtc
    12121 gctcaccatg gccagcgtcg acagcggcaa cgatgacacc atgctggcgc tataccagca
    12181 caaggagtgg tggatgcgcc cgacctgggt gcgtacacca tttgaactgc ctgaacgcac
    12241 gcagttattg ttatgtcgca acaacgatgc cgaagatgcc gagtggctag tgttggtcgc
    12301 tatctgcggc acggatattc gggctgattt cagcgggcag cccgcaacag aatctgatga
    12361 cacggctttg cggctcgtgc tgtcttccaa ccgggttggt cgcacgactc tatgtgacac
    12421 tgccgcatat atcgcttgcg catccgaccc gtatatggcc attcgagctg ccacacaaac
    12481 ggcagcacgc cagctgggta ttcgcacccg taaggagcgt ccattcccgg atgcgctcac
    12541 cgggttggga tggtgtactt gggattcatt ggggcgagat gtcaacgagc aggccatcgt
    12601 caacaagatg gaggagttcc aggccaagca tgtgcccatt tcctgggtgc ttatcgatga
    12661 cggttggtcc aacacggatc gcacaaagga aaccctgatc gactttggcg cggatcgcca
    12721 acgtttcccc cacgggcttg cccacaccat tgcgctgctc aaaacgcact atggcgtgcg
    12781 cagcgtaggc gtgtggcagg cgttccaagg ctattggaat ggtttggacg agtcgggtgt
    12841 agccgcggcc agctgcccga ccgcaataac caccacggcg aatggttgcc tgattcctgg
    12901 aagtcgtgct gaacaacccg cacagttctg ggatgcctgg gacggcgaac tcgccgaagc
    12961 cggcgtcgat ttcgtcaaag tggattcgca aagttctacg tcagtaatgg ttcgtggcgc
    13021 cgaaagttac ggtgaagcca cctggggacg ccatcaggcg cttgatgaag tgacctcacg
    13081 acgattcggc ggcgcattga ttaactgcat gggcatggca ccggaggatt attggcatcg
    13141 tccttcatcg ccaatcaccc gtagtagcga cgactatctg ccgcacaatc ctgattcgct
    13201 gggcgaacac cttatccaga acgcatactg tgcactgctg atgggggagc tgtaccactg
    13261 tgattgggac atgttctgga cggagcatcc ccatgcgcga gtccatgccg tgctccgatt
    13321 gcttagcggc gggccagtgt attgttccga tgcctgcggt cacactgatg cggctgtctt
    13381 gcgtgattta cttgccgaag atggtacatt accgcgtcct gatgagccgg cccgaccagt
    13441 cattgcgtca ctgctcaatg accccgaaca tgcgggctat gcgctcggtg tcacagcaag
    13501 attcggcgca gaacaggtca tcgctttcgt cggattacgc cggcagccac aaatcggcat
    13561 cattacggct agcggtcgct cttgccgcat catcgatgac catggtgcga cgattgatat
    13621 gaatccgggg gaaacccata cttgcgagat cgagtacggc caactgcgtg ttttccgggt
    13681 gcaatcatga acgcgcatat gccgattgat atttattttc cggcacacca acgcagttcc
    13741 ccaatgggaa tgtaattcgc caatttttag actgtgagca acgagcaata tagatatcaa
    13801 taaggagcca atgatgacta ctttcaaccg cgcaataatt cccgacgcca tccgcaccaa
    13861 cggcgccacc cccaatccgt ggtggtccaa cgcggtggtc tatcagattt acccgcgttc
    13921 tttccaagac acgaacggcg atggattcgg cgatcttaag ggcatcacgt cgcgtcttga
    13981 ttacttagct gatcttggcg tggatgtgct gtggctctcc ccggtctata agtccccgca
    14041 agacgacaac ggctatgaca tctctgacta tcaggacatc gacccgctgt tcggcacgct
    14101 cgacgatatg gacgagctgc tggccgaagc gcataagcgt gggctcaaag tcgtgatgga
    14161 tttggtggtc aatcacacct ccgatgagca tgcctggttt gaggcgtcca agaacaagga
    14221 cgacgagcat gccgattggt attggtggcg tccggctcgt cccggcacca cgcccggcga
    14281 gcccggctcc gagcccaatc agtggggctc ctactttggc ggttccgcat gggaatattg
    14341 ccccgagcgt ggtgagtact atctccacca gttctcgaag aagcagcccg atctgaactg
    14401 ggagaacccg gccgtgcgcc gagccgtgta cgacatgatg aactggtggc ttgaccgagg
    14461 catcgacggc ttccgcatgg atgtcatcac cctgatctcc aagcgtacgg atgcaaacgg
    14521 caggctgccc ggcgagtacg gttccgagct ggacgatctg cctgtgggcg aggaaggcta
    14581 ttccaatccc aacccgttct gtgccgatgg gccgcgccaa gacgagttct tgaaggaaat
    14641 gcgtcgtgaa gtctttgccg gacgcgaggg attcctcacc gtgggcgagg ctcccggcat
    14701 cacacctgtg cgcaacgaac acatcaccaa tccggccaat ggggagctgg atatgctgtt
    14761 cctgttcgat catgtcgatt ttgattgtga tggcgtcaag tggaagcctc tgccgctcga
    14821 tttgccggga ttcaagcgga tcatggccgg atatcagact gctgtggaga acgtgggctg
    14881 ggcaagcttg ttcactggta accacgatca gccacgtgtg gtctctcgtt ggggcgatga
    14941 ctcctcggag gaatcccgcg tgcgctcggc caaagcgctt ggcctgatgt tgcacatgca
    15001 tcgcggcact ccgtacgtat atcagggtga ggagctgggc atgaccaatg ctcacttcac
    15061 cagcctcgat cagtaccgcg accttgaatc cctcaatgcc tatcgtcaga gggtcgagga
    15121 agccaaggtg caatcgccgg aatcgatgtt ggcgggtatc gccgcgcgcg gtcgcgacaa
    15181 ttcgcgtacc ccaatgcaat gggatggttc tgcctatgca ggtttcaccg caccggatgc
    15241 agcgacggag ccgtggattt ccgtcaaccc gaatcatgct gaaatcaatg cggccggcga
    15301 atttgacgat cctgactcgg tgtatgcctt ctacaagaag ctcatcgcct tgcgccacaa
    15361 cagttcgatt gtggcggctg gcgagtggca gctgattgat gcggatgacg cgcatgtata
    15421 tgcgttcacc cgcacgcttg gcaacgagcg attgctggtt gtggttaacc tgtccggccg
    15481 aaccgtcgac ttgccgcgtg aatccaccga gctgattgcc ggcggcgtca ctgagccaga
    15541 tatcattctc tccacgtacg acgcccctca cactgtggtc tccctcgcca accgtgagct
    15601 tgacccgtgg gaggctgctg ccgtccagct gtaagtaggt atttttgttt caaggggctg
    15661 gtgctcaaat agttcagggt ctgaaaccgc ataataccgt cgtaaataat gcgagatact
    15721 tggggtatac cgaattaagc agcccctcga aacggagata gacgtctgga aacggtcaaa
    15781 tccgcccctc gaaagcggaa aatacggtag gactgcgcca atgattgctc tgaagtatct
    15841 tgtcaacgag atgaatagtc attttcggcg tccccgcgcc atacaatgag cagctatgga
    15901 acaaaaggat gttttgaagg cgctgcagcg tgatcatgca gccgaactga agctggcctc
    15961 cgaacgactc aaaggcactg cccgtcacac ggagattatt ccttccccgg tgttgagcga
    16021 gatgactggc cacgagattc tcctgaagcc tgagaatctg caggtcactg ggtcattcaa
    16081 gattcgtggt gcatataaca agattgcttc gctcgccgaa gagcagcttt ctcatggtat
    16141 cgtcaccgca tccgctggca accatgcgca aggcgtggcc tatgcagccc gggaacgtgg
    16201 cgccaaggcc actatctgca tgccgcagat tactccgccg ctgaaagtgg atgccaccaa
    16261 ggcctatggt gccgatgtgg tgctctacgg tgatgtgttc gatgaatccg cagcgcatgc
    16321 cgccgaactc gccgagacga agggcatgat ctacgtgccg ccgttcgatg actacgaggt
    16381 catttgcggt caaggcacca ttgggttgga gattctcgaa gatgtgccga atgtcaccga
    16441 tgttgtggtg ccgctcggtg gtggcggtct tggcgccggt gtggcgctcg ccatcaagac
    16501 cttcaaacct gatgttcgtg tgattggcgc aattccggaa ggttctccgg cctggaagga
    16561 ttcgttggca tctggccatg tatcccccgc cgatcgtgtg gtgacttccg ccgagggcgt
    16621 tgcggtcaag catccgggtg atttgccgtt tgcattgctc aacgagttcc tggatgattt
    16681 ggtcactgtc tccgagcgag acatcaacga gatgattctg ctcatgctgg agaagcacaa
    16741 gttggtggtc gaggccgctg gtgccgtctc gctggcagcg ctcgagcatc tgaatttgcg
    16801 ttcccgcaag ttcgctgcgg ccactggtcc gcatgtggta gtgccgatta tgtcaggcgg
    16861 caacatcgat accgtgacca tcggcgccgt gattcaaaag ggtatgattg cccgcggccg
    16921 catcatgaac tttgaggtcg agctgccgga taccccgggc cagttggtca aggtggccac
    16981 gttgcttgcc gagcagcgcg ccaacgtcat ccaactggat cacgatcagt tcaaggcctc
    17041 cggccattac accaacgcgg tatctcttgg tgtcacggtc gaaaccaacg gccctgacca
    17101 tattgaccgc atccttgcgg ctctgcgcga agctggcttt agccctaagc gcatctactg
    17161 atgtcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat
    17221 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
    17281 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
    17341 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
    17401 ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac ggcgacaagc
    17461 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
    17521 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
    17581 ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
    17641 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
    17701 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
    17761 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
    17821 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
    17881 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
    17941 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
    18001 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
    18061 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
    18121 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
    18181 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
    18241 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
    18301 tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga cgctttttat
    18361 cgcaactctc tactgtttct ccatacccgg atcttacagt gaggcataat tatggctttc
    18421 attccaccaa ccatcgacga cgttagacat tgctctaacg ctttatctgt agaccccgcc
    18481 gaaaccgacg ctgcccgcgc cattgctgaa cactactcaa agatatccaa tcaggagtac
    18541 cgcatcaccc aagacgacct ggatgatctc actgacacaa tcgaatatct catggccact
    18601 aaccagccag actcacaata aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
    18661 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
    18721 ctggctcacc ttcgggtggg cctttctgcg tttataggat ctctaatagg ctatgtacgc
    18781 gtatcaacaa atgaacaaaa cactgcttta caacgaaacg ctcttgaaag cgcaggatgt
    18841 gagctaattt ttgaggacaa ggcgagcggc aaaaaggctg agcgccctgg gttaaaaaag
    18901 gtactccgta actttccaga ggtgataccc tggtcgtatg gaagttagac cgtcttgggc
    18961 gcagcatgcg tcacttagtt gtgctggtgg aagagctgcg tgacagaggc attaacttcc
    19021 ggagtctcac tgactccatc gacaccagta caccaatggg gcgctttttc tttcacgtaa
    19081 tgggggcgct ggcagaaatg gaacgtgagc ttatcgttga acgtacacgc gctggacttg
    19141 atgcagctcg cgcagaaggt cgtataggtg ggcgtcggcc taaataccaa gaagaaacat
    19201 ggcagcaaat gcggcgattg ctggagaagg gcatcccccg taagcaggtt gcaatcatct
    19261 atgatgtggc tgtttccacg ctttataaga agtttccggc gtcatcattt caatcctaaa
    19321 ccttggttta agagaactcg gggatcttaa agcgatttta ctttttctgg ctatgaattg
    19381 gcctgctttg taacacactc cggtctatcc cgtagcgccg ggcatatcct gtcgcaatgt
    19441 gcaaatctcg cggcaacaac cagtgaatac ttcattcaca agcctcaccg cctgatcgcg
    19501 gcagaaactg gttatagcca atcaaccgtc gttcgtgcat tccgtgaagc tgtaaacaaa
    19561 ggtattctgt ctgtagagat tgttatcggc gatcaccgtg aacgtcgcgc taacctgtac
    19621 cggtttacac catccttttt ggccttcgca caacaagcca aaaatgcgct gatagaaagc
    19681 aaattaaaga tttcgtcagc ggcaaccaag gttaaagctg ttctcgctaa gacattggct
    19741 ttatttaatt ttttatccac acccccatgt caaaatgata ccccctcccc ctgtcaggat
    19801 gacgtggcaa taaagaataa gaagtcacaa gttaaaaaaa caaaaagatc agtttccggc
    19861 ggtgccggaa caaccagcct caaaaaattg acttcatgga tcgctaaggc aaaagcaaag
    19921 gctgacaatc tgcggttatc caaaaaacgc actcaaaaac atgagttcaa gcagaaagta
    19981 gaggcggctg cgcggaaata tgcttacctg aaaaacaagc gttcgcctga tattggcggg
    20041 atatcaaact tcgataacct accgcattgc atgacggtaa acgaagctct taatgcggtt
    20101 ttagccaaaa ataaagataa cgaacaatgg ggtataccgg caggattcag agggtaagga
    20161 tctgaatctg gtgtgttaac cgattctacg agtagtcatt tgttcattga gtaggaatat
    20221 tgttgggatc tcgaaaggaa gcataagtga cctgggacga tcacaagaag aattttgctc
    20281 gcctggcgcg agatggtggt tacaccatcg cacagtatgc cgccgagttt aatcttaacc
    20341 ctaataccgc acgtcgttat ctccgtgcct tcaaagaaga caccaggact acggacagcc
    20401 gcaagccaaa taagccagtc aggaagccac taaaaagcat gatcattgat cactctaatg
    20461 atcaacatgc aggtgatcac attgcggctg aaatagcgga aaaacaaaga gttaatgccg
    20521 ttgtcagtgc cgcagtcgag aatgcgaagc gccaaaataa gcgcataaat gatcgttcag
    20581 atgatcatga cgtgatcacc cgcgcccacc ggaccttacg tgatcgcctg gagcgcgaca
    20641 ccctggatga tgatggtgaa cgctttgagt tcgaagttgg cgattacctg atagataacg
    20701 ttgaagcgcg gaaggccgcg cgcgctatgt tgcgtcggtc cggggccgat gttctggaaa
    20761 ccactcttct ggaaaagtct ctttctcatc tccttatgct ggagaacgcc agggatacgt
    20821 gtattcgcct ggtgcaggaa atgcgcgatc agcaaaaaga cgatgatgaa ggtactccgc
    20881 ctgaataccg tatcgcgagc atgctaaaca gctgttccgc gcagataagc agcctgatca
    20941 acaccattta cagcatccgg aataactatc gaaaagaaag ccgggaggcg gaaaagcacg
    21001 ctttatctat ggggcaagct ggcattgtta agctggcata cgaacgaaag cgtgaaaata
    21061 actggtcagt gctggaagcg gctgagttca tcgaggcgca tggaggaaaa gtgccgcccc
    21121 tgatgctgga gcaaatcaaa gccgatctgc gtgctcctaa gaccaatacc gatgatgagg
    21181 aaaaccaaac agcatctggc gctccatcac ttgaggatct ggataaaatc acgcgagaac
    21241 gggccgccag ccgccgcgct gatgccgcat tgtggattga gcatcgtaga gaagaaattg
    21301 ccgatatcgt cgatacaggt ggttatggtg atgtcgatgc ggaaggcata tcaaacgaag
    21361 catggcttga acaggatctg gacgaagacg aggaggaaga cgaagaagtt acccgcaaac
    21421 tgtacgggga tgatgattaa ggatctggcg attgctggcc tagatgaatg actgtccacg
    21481 acagaacccg gcttatcggt cagtttcacc tgatttacgt aaaaacccgc ttcggcgggt
    21541 ttttgctttt ggaggggcag aaagatgaat gactgtccac gacgctatac ccaaaagaaa
    21601 gcggcttatc ggtcagtttc acctggttta cgtaaaaacc cgcttcggcg ggtttttgct
    21661 tttggagggg cagaaagatg aatgactgtc cacgacacta tacccaaaag aaagcggctt
    21721 atcggtcagt ttcacctgtt ttacgtaaaa acccgcttcg gcgggttttt acttttggag
    21781 gggcagaaag atgaatgact gtccacgaca ctatacccaa aagaaagcgg cttatcggtc
    21841 agttttacct gatgtacgta ataaaccgtt cggatctcaa ttcgacccag ctttcttgta
    21901 caaagtggtt gatccttaca gatcccggtt atccacagaa tcaggggagg cctctagaaa
    21961 tattttatct gattaataag atgatcttc
//
