LOCUS       sequence-48283-e        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pBad-Xis/Int Reset Flipper
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(78..121)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    124..145
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(146..1024)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1051..1334
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1257..1276
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2194..3693
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     misc_feature    3737..3757
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3758..3815
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3839..3856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4146..4165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4762..4856)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4877..5536)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5451..5470
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5537..5639)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctggaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtcacattga ttatttgcac ggcgtcacac tttgctatgc
     1261 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     1321 tactgtttct ccataccgtt tttttgggct agctactaga ccttaacaaa ctacggaggt
     1381 caacatggcc ggcactcagc gtatcgtctt tctacccgat actcagttgc ctttcgaggc
     1441 gcgcaaagag atgcaagcgg tcatccgctt catcggggat gtccagccgt acggcgtggt
     1501 acatatcggt gacgtcctag acctgccgca gccctcgcgc tggaaccggg gaaccaaggg
     1561 cgagttcgag ggctcggtgt accgcgacgc ggactacgcc aagaagaacc tgatggagcc
     1621 gctgcgcaag gtctacgacg gctggatcgg gatgcacgag ggcaaccacg atctgcgagc
     1681 ccgcgagtac ctggccaaga acgcaccggc cctggagggt acgcacgctt tcgacatcga
     1741 cgtgctgctc gacttcgacg ggttcggtgt ggagctgctg cctgacttct acgacatcgc
     1801 tccgggctgg atctccactc acgggcacat gggcaagatg acgctatccc agatcgccgg
     1861 atcgacagcg ctcaacggtg ccaagaagtt cggcaagtcc gtggtctgcg gccacacgca
     1921 ccggcaggct gtcgtctcgc actcgttcgg gtacggcggc tcggtgcgca agaccgtcac
     1981 cggcatggaa gtcgggcacc tgatggacat gaagaaggcc aactatctaa agggcggagc
     2041 cgggaactgg cagatgggct tcgggatgct cacggtcgac ggcaagcatg tcaaggctga
     2101 gatcgtcccg atcctgggag gcaagttcac cgttgacggc caggtctggg aagtcaccgg
     2161 ttaatactag tcacacagga aacctactag gtgagagccc tggtagtcat ccgcctgtcc
     2221 cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg agtcttgcca gcagctctgc
     2281 gcccagcgcg gctgggacgt cgtcggggta gcggaggatc tggacgtctc cggggcggtc
     2341 gatccgttcg accggaagcg cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa
     2401 ccgtttgacg tgatcgtggc gtaccgggta gatcggttga cccgatcgat ccggcatctt
     2461 cagcagctgg tccactgggc cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg
     2521 cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg
     2581 cagatggaat tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt caatatccgc
     2641 gccgggaaat accgaggatc cctgccgccg tggggatacc tgcctacgcg cgtggacggg
     2701 gagtggcggc tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc
     2761 gtcgtcgaca accacgagcc gctgcatctg gtggcccacg acctgaaccg gcgtggtgtc
     2821 ctgtcgccga aggactactt cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg
     2881 tcggctaccg cgctgaagcg atcgatgatc tccgaggcga tgctcgggta cgcgactctg
     2941 aacggtaaga ccgtccgaga cgacgacgga gccccgctgg tgcgggctga gccgatcctg
     3001 acccgtgagc agctggaggc gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc
     3061 gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc
     3121 gcgtacaagt tcgccggggg aggacgtaag cacccgcgct accgctgccg ctcgatgggg
     3181 ttcccgaagc actgcgggaa cggcacggtg gcgatggccg agtgggacgc gttctgcgag
     3241 gagcaggtac tggatctgct cggggacgcg gagcgtctgg agaaagtctg ggtagcgggc
     3301 tcggactccg cggtcgaact cgcggaggtg aacgcggagc tggtggacct gacgtcgctg
     3361 atcggctccc cggcctaccg ggcgggctct ccgcagcgag aagcactgga tgcccgtatt
     3421 gcggcgctgg ccgcgcggca agaggagctg gagggcctgg aggctcgccc gtctggctgg
     3481 gagtggcgcg agaccgggca gcggttcggg gactggtggc gggagcagga caccgcggca
     3541 aagaacacct ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg
     3601 actcgcacga tcgacttcgg ggatcttcag gagtacgagc agcatctcag gctcggcagc
     3661 gtggtcgaac ggctacacac cgggatgtcg taataatact agcgcaaaaa accccgcttc
     3721 ggcggggttt tttcgctact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt
     3781 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
     3841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3901 aggcggtaat cctagctagg gcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4261 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4381 caatgccacc tgacgtctaa gaacccggta agacacgact tatcgccact ggcagcagcc
     4441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4501 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4741 ttggtcatga cttgtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     4801 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     4861 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     4921 taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
     4981 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     5041 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     5101 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     5161 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     5221 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     5281 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     5341 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     5401 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     5461 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     5521 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     5581 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     5641 cgtctcattt tcgccagata tcgacgtcta agaaaccatt attatcatga cattaaccta
     5701 taaaaatagg cgtatcacga ggccctttcg tcttcacc
//
