LOCUS       sequence-483049-        3513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schistosoma mansoni h2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Lee J, Chong T, Newmark PA
  TITLE     The esophageal gland mediates host immune evasion by the human 
            parasite Schistosoma mansoni.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):19299-19309. doi: 
            10.1073/pnas.2006553117. Epub 2020 Jul 31.
  PUBMED    32737161
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2006553117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-08-11- 11"; volume: "117"; issue: "32"; pages: 
            "19299-19309"
COMMENT     Sequence Label: pJL_h2b
FEATURES             Location/Qualifiers
     source          1..3513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(393..411)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(394..411)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(415..433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      447..494
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(506..523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(677..1265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(757..776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1436..2227,2228..2296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2059..2078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2314..3108)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2425..2444)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3035..3054
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct tggaaggaga
      121 gttatgctat ctacatctac aaagtccttc gtcaagtcca tcctgacact ggtatctcgt
      181 cgaaggcgat gtcgatcatg aactcgtttg tgaacgatat ctttgagcgc attgccgcag
      241 aggccagccg actatcacac tacaacaaga agtcgacaat ttcaagccga gaggtgcaga
      301 cagcggttcg gttgttgttg ccaggtgaat tggcgaaaca cgcggtatct gaaggcacga
      361 aggcagtgac gaagtacagt gggaaggtca cttctatagt gtcacctaaa tctccctata
      421 gtgagtcgta ttaggatccg gcgcgcctag cataacccct tggggcctct aaacgggtct
      481 tgaggggttt tttgacgcgt gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1501 cctggctccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2281 aatgttgaat actcatactc ttcctttttc aattcagaag aactcgtcaa gaaggcgata
     2341 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     2401 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     2461 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     2521 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     2581 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     2641 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     2701 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     2761 catgatggat actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac
     2821 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     2881 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     2941 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     3001 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     3061 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     3121 tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatcattg gcggcaagaa
     3181 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     3241 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     3301 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     3361 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct
     3421 ttagcagccc ttgcgccctg aattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3481 tcatttttta accaataggc cgaaatcgac gcg
//
