LOCUS       sequence-483078-        4176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR13_H2_b4gat1
FEATURES             Location/Qualifiers
     source          1..4176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1056..1074)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1057..1074)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1078..1096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1110..1157
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1169..1186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1340..1928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1420..1439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2099..2890,2891..2959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2722..2741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2977..3771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3088..3107)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3698..3717
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct tcatacccgc
      121 aagatgttcc tattcacact attattgaac aagtcaaggc tggtctgcct gtttcctata
      181 aaccaaaata taaccatccc atcaaaatac ttcgaacaag tggttctgtg tgctctggtg
      241 tcacaagaca caatcttgtc gtcgtcgtta agagtggagt ccacaacttt gcagcgagag
      301 aagaattcag gactttcatg aagaaccaaa ccctctcgtg tccttttttc agagtcggtt
      361 atgtattcag tgttggatta cctcgggcac acggaggtcg aattttcaac cgagatggaa
      421 acattgtgaa cttgcctggt gcagctggcg atgcgcttga gaaatatgat ggaaagtcaa
      481 atattttaat ggatgaaata aacaaggaaa tagacaagta tgacgacctt atacttggag
      541 attacgagga tacatatttc aatctctcgt ggaaaacggt taccaatctc cgttggattt
      601 cagcattttg tagtaagaac caaggagatt tctttatgat tatcgatgat gatcacagaa
      661 tgaatttggc gctagtgagg aactttgcga aggaggtgaa aacctcgtat ctaagaacaa
      721 gtatacatgg aaaaattaga ggtcatgaca aggcgattag atctccaagt tcgaaaaatt
      781 tcgtatcgta tcgtgagata ccatgggatg atgcattacc ttatcccatg ggtatgtccc
      841 aactgattgg agccgatatt gtggatgata tggccattgc aacagcctac acaaagtacg
      901 cttatttgaa tgaagacgtt tatcttgggc tgctagccca taaactggga attactatac
      961 gaaatgtcgt cagcatgttt aatcacgatg aatacaaaaa ttacgagaag aaatattccc
     1021 ctatggttgc ttggaagcgg tattttaagg tcacttctat agtgtcacct aaatctccct
     1081 atagtgagtc gtattaggat ccggcgcgcc tagcataacc ccttggggcc tctaaacggg
     1141 tcttgagggg ttttttgacg cgtgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1741 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2161 ttgcctggct ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2941 ggaaatgttg aatactcata ctcttccttt ttcaattcag aagaactcgt caagaaggcg
     3001 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3061 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3121 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3181 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3241 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3301 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3361 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3421 agccatgatg gatactttct cggcaggagc aaggtgggat gacaggagat cctgccccgg
     3481 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3541 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3601 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     3661 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     3721 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     3781 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatca ttggcggcaa
     3841 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     3901 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     3961 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     4021 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     4081 cctttagcag cccttgcgcc ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc
     4141 agctcatttt ttaaccaata ggccgaaatc gacgcg
//
