LOCUS       sequence-48328-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of LC3 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos 
            M, Alt FW, Finkel T
  TITLE     A role for the NAD-dependent deacetylase Sirt1 in the regulation of 
            autophagy.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9.
  PUBMED    18296641
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Mar 4. 105(9):3374-9."
COMMENT     Sequence Label: pEGFP-LC3 (human)
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..422
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     polyA_signal    554..675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(591..610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     645..664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(819..840)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agtccggccg gactcagatc tcgagctcaa gcttcgaatt cccgaccatg ccgtcggaga
       61 agaccttcaa gcagcgccgc accttcgaac aaagagtaga agatgtccga cttattcgag
      121 agcagcatcc aaccaaaatc ccggtgataa tagaacgata caagggtgag aagcagcttc
      181 ctgttctgga taaaacaaag ttccttgtac ctgaccatgt caacatgagt gagctcatca
      241 agataattag aaggcgctta cagctcaatg ctaatcaggc cttcttcctg ttggtgaacg
      301 gacacagcat ggtcagcgtc tccacaccaa tctcagaggt gtatgagagt gagaaagatg
      361 aagatggatt cctgtacatg gtctatgcct cccaggagac gttcgggatg aaattgtcag
      421 tgcaggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
      481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      661 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      721 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      781 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      841 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaa
//
