LOCUS       sequence-48334-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pTK14
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..80
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             93..137
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc cgctctagag
       61 agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac
      121 gccagcacat ggacagcgga ggtggaggta ctagtctcga gatgagagaa gaccattctt
      181 ttcatgttcg ttacagaatg gaagcttctt gcctagagct ggccttggaa ggggaacgtc
      241 tatgtaaatc aggagactgc cgcgctggcg tgtcattctt tgaagctgca gttcaagttg
      301 gaactgaaga cctaaaaaca cttagcgcta tttacagcca gttgggcaat gcttatttct
      361 atttgcatga ttatgccaaa gcattagaat atcaccatca tgatttaacc cttgcaagga
      421 ctattggaga ccagctgggg gaagcgaaag ctagtggtaa tctgggaaac accttaaaag
      481 ttcttgggaa ttttgacgaa gccatagttt gttgtcagcg acacctagat atttccagag
      541 agcttaatga caaggtggga gaagcaagag cactttacaa tcttgggaat gtgtatcatg
      601 ccaaagggaa aagttttggt tgccctggtc cccaggatgt aggagaattt ccagaagaag
      661 tgagagatgc tctgcaggca gccgtggatt tttatgagga aaacctatca ttagtgactg
      721 ctttgggtga ccgagcggca caaggacgtg cctttggaaa tcttggaaac acacattacc
      781 tccttggcaa cttcagggat gcagttatag ctcatgagca gcgtctcctt attgcaaaag
      841 aatttggaga taaagcagct gaa
//
