LOCUS       sequence-483595-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Kva1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Kva1
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            247..355
                     /note="incP origin of transfer"
     CDS             complement(366..1217)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1204..2043)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2554..3525)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      3632..4026
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4064..4150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4175..5140)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        6040..6120
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             7505..8290
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     terminator      8918..9012
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9133..9152)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(9144..9879,1..80)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gtctgtttca gaaatatggc attgataatc cggatatgaa caaactgcaa tttcatctga
       61 tgctggatga atttttctaa taattaattg gaccgcggtc cgcgcgttgt ccttttccgc
      121 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
      181 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
      241 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
      301 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc
      361 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
      421 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
      481 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
      541 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
      601 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
      661 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
      721 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
      781 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
      841 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
      901 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
      961 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
     1021 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
     1081 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
     1141 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
     1201 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
     1261 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
     1321 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     1381 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     1441 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     1501 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     1561 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     1621 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     1681 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     1741 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     1801 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     1861 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     1921 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     1981 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     2041 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     2101 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     2161 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     2221 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     2281 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     2341 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     2401 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     2461 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     2521 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     2581 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     2641 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     2701 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     2761 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     2821 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     2881 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     2941 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     3001 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     3061 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     3121 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     3181 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     3241 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     3301 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     3361 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     3421 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     3481 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga
     3541 ttcccacaca ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg
     3601 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     3661 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     3721 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     3781 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     3841 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     3901 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     3961 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     4021 ggttcgtgac agttagggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     4081 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     4141 gttttatttg atgcctttaa ttaaacgttc gtaatcaagc cacttccttt ttgcattgac
     4201 gcagggtgtc ggaaggcaac tcgccgaacg cgctcctata gttttcagcg aagcgtccca
     4261 aatgtaagaa gccgtagtct agggctatct cagttatact acgcacattg gcactgggat
     4321 cgttcaagca ggcgcggatg ctttcgagct tgcggttgcg gatgtagttc ttcggcgtgg
     4381 tgccggcgtg cttctcgaac aaattgtaga gcgagcgtgg actcatcatc gccagctccg
     4441 ctaaccgctc aaggctgata ttccgtttga gattctcctc aatgaattga acgactcgct
     4501 cgaaagacgg gttacctttg ctgaaaattt cacggctgac attgctgccc agcatttcga
     4561 gcagcttgga agcgatgatc cccgcatagt gctcttggac ccgaggcatc gactttgtat
     4621 gttccgcttc gtcacaaact aacccgagta gattgataaa gccatcgagt tgctggagat
     4681 tgtgtcgcgc ggcgaaacgg ataccctccc tcggcttgtg ccaattgttg tcactgcacg
     4741 cccgatcaag gaccactgag ggcaatttaa cgataaattt ctcgcaatct tctgaatagg
     4801 tcaggtcggc ttggtcatcc ggattgagca gcaatagttc gcccggcgca aaatagtgct
     4861 cctggccatg gccacgccac aggcaatggc ctttgagtat tatttgcaga tgataacagg
     4921 tctctaatcc aggcgagatt accctcacgc taccgccgta gctgattcga cacagatcga
     4981 ggcatccgaa gattctgtgg tgcagcctgc ctgccgggcg cccgcccttg ggcaggcgaa
     5041 tagagtgcgt accgacatac tggttaacat aatcggagac tgcatagggc tcggcgtgga
     5101 cgaagatctg acttttctcg ttcaataagc aaaaatccat agttcacggt tctcttattt
     5161 taatgtgggc tgcttggtgt gatgtagaaa ggcgccaagt cgatgaaaat gcatctcgac
     5221 gtgatgcgta tacgggttac ccccattgcc acgttgcgcc atcctttttg caatcagtga
     5281 ccacttttcc aagcaaaaat aacgccaagc agaacgaaga cgttcttttt aagaagcgag
     5341 aacaccagaa gttcgtgctg tcggggcatg gggcgacgaa ttggcggata aaggggatct
     5401 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     5461 ctttattcac attcttgccc gcctgatgaa tgctcatccg taattacgta tggcaatgaa
     5521 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     5581 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     5641 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     5701 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     5761 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgcatg ggaattagct
     5821 tgatctgacc aacgaccggt agcggagcta tccaacggcg gtataccagg aaaacacaca
     5881 gcaggtacat cagaacagta ccatgactga agaacaaata gttttttcct gatccataaa
     5941 gcagaacggc ctgctccatg acaaatctgg ctccccaact aatgccccat gcagccagca
     6001 taaccagcat aaagtgcagt gtccggtttg atagggataa gtccagcctt gcaagaagcg
     6061 gatacaggag tgcaaaaaat ggctatctct agtaaggcct accccttagg ctttatgcaa
     6121 cctctaggat ccgaattcga aggagatata catatgtcct atatcgatga gctgagcaag
     6181 actctgaact gcactccgaa gactattatc tctggctaca tcaaggcgcc taagaaatac
     6241 aaggtgttct ttatcaaaaa gaaaaacggt aaaggcctcc gtgagatcgc gcagccgtct
     6301 ccggacgtta agaaagttca acgtgctatt gttaacagcc tcctgtctct cctgccggtt
     6361 cacgagtgcg cgaccgcgta tatcgaggaa aaatccattg ttgataacgc agaaccgcac
     6421 aagaaaaacg aatacatcct caaaatggat ttcgaaaatt ttttctacag catcaaaccg
     6481 caggatctga actattttat cagcaaaaac gatattaaac tgaaccaatt tgaatatgaa
     6541 gtaattagcc actacctgtt tcgtcgcaag aaaggtgaaa agaaactgcg cctgtgcatc
     6601 ggtgcgccga gctctccgat gatctccaac atcgttatgt acgacttcga cgaaaaactg
     6661 ttcaactact gtaaagaaaa cgatgtgatc tacactcgtt acgctgatga cctgtctttt
     6721 tcttccgatt cttttgacaa actgaacgat gtcaaaaact tcgttattaa cctggtgtct
     6781 ctgcagaaga aaccagaact gattatcaac acttctaaaa ccaaatttat tggtaaaggt
     6841 cgtagccgtc gcattactgg tgtggttatt tctaacaccg gcgatctggg tgtgggccgt
     6901 tttctgcgta agaaaatccg cgcactgttt tacctgtaca gcaataacaa aatcaatcat
     6961 aaggacattc cgtacctcct gggcatgctg tctcacatca aaaatatcga accggactat
     7021 tactatagcc tgattaaaat taacggtgag gacaaactga aacaactggg taaggatagc
     7081 ttcgcagtcc tgcagaaaaa caatgactaa taccgttcat atgaggctag agcaggtgat
     7141 ccataatctt gagacaagca gcttgattga tgcaaatcat taagttagtg tataacatac
     7201 tctttacgcg atagaggcgt atctgcttca tctggtgtat caccggaact tgttgattgt
     7261 ggtcatttat gccacggcct aagtgaagag cgagtgtgag ccgttgtagc ttcaacctgt
     7321 cccttacgaa tagctcttca cccaggccgt gcccttctag gtaaagagca gaaccccggt
     7381 ttaccggggt tttttataac ataagcggct gccacctgca tgatccgcgg ccgctaaaaa
     7441 ggagatcaac aatgaaagca attttcgtac tgaaacatct taatcatgct atggaggttt
     7501 tctaatgagt actgcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga
     7561 ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc
     7621 cagcgatgcg cagttcatcg cattactgat cgttgccaac cagtacggcc ttaatccgtg
     7681 gacgaaagaa atttacgcct ttcctgataa gcagaatggc atcgttccgg tggtgggcgt
     7741 tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg actttgagca
     7801 ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt
     7861 taccgaatgg atggatgaat gccgccgcga accattcaaa actcgcgaag gcagagaaat
     7921 cacggggccg tggcagtcgc atcccaaacg gatgttacgt cataaagcca tgattcagtg
     7981 tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt
     8041 cgaaaatact gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga
     8101 tgaaaccatg caggagatta acactctgct gatcgccctg gataaaacat gggatgacga
     8161 cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt cagaactgac
     8221 acaggccgaa gcagtaaaag ctcttggatt cctgaaacag aaagccgcag agcagaaggt
     8281 ggcagcatga ggatccagga gaatacacat ggccagcaga ggcgtaaaca aggttattct
     8341 cgttggtaat ctgggtcagg acccggaagt acgctacatg ccaaatggtg gcgcagttgc
     8401 caacattacg ctggctactt ccgaatcctg gcgtgataaa gcgaccggcg agatgaaaga
     8461 gcagactgaa tggcaccgcg ttgtgctgtt cggcaaactg gcagaagtgg ccagcgaata
     8521 tctgcgtaaa ggttctcagg tttatatcga aggtcagctg cgtacccgta aatggaccga
     8581 tcaatccggt caggatcgct acaccacaga agtcgtggtg aacgttggcg gcaccatgca
     8641 gatgctgggt ggtcgtcagg gtggtggcgc tccggcaggt ggtaatatcg gtggtggtca
     8701 gccgcagggc ggttggggtc agcctcagca gccgcagggt ggcaatcagt tcagcggcgg
     8761 cgcgcagtct cgcccgcagc agtccgctcc ggcagcgccg tctaacgagc cgccgatgga
     8821 ctttgatgat gacattccgt tctgatctag agtcgacctg caggcatgca agcttgcggc
     8881 cgcgtcgtga ctgggaaaac cctggcgact agtcttggac tcctgttgat agatccagta
     8941 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     9001 attggtgaga atccaggggt ccccaataat tacgatttaa atttgtgtct caaaatctct
     9061 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     9121 taaacagtaa tacaaggggt gttatgagcc atattcagcg tgaaacgagc tgtagccgtc
     9181 cgcgtctgaa cagcaacatg gatgcggatc tgtatggcta taaatgggcg cgtgataacg
     9241 tgggtcagag cggcgcgacc atttatcgtc tgtatggcaa accggatgcg ccggaactgt
     9301 ttctgaaaca tggcaaaggc agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga
     9361 actggctgac cgaatttatg ccgctgccga ccattaaaca ttttattcgc accccggatg
     9421 atgcgtggct gctgaccacc gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat
     9481 atccggatag cggcgaaaac attgtggatg cgctggccgt gtttctgcgt cgtctgcata
     9541 gcattccggt gtgcaactgc ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc
     9601 agagccgtat gaacaacggc ctggtggatg cgagcgattt tgatgatgaa cgtaacggct
     9661 ggccggtgga acaggtgtgg aaagaaatgc ataaactgct gccgtttagc ccggatagcg
     9721 tggtgaccca cggcgatttt agcctggata acctgatttt cgatgaaggc aaactgattg
     9781 gctgcattga tgtgggccgt gtgggcattg cggatcgtta tcaggatctg gccattctgt
     9841 ggaactgcct gggcgaattt agcccgagcc tgcaaaaac
//
