LOCUS       sequence-483867-       12658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express H3-mRFP1 in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 12658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY225
FEATURES             Location/Qualifiers
     source          1..12658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1727..2401
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             1727..2401
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(1854..1872)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2122..2141
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2316..2339
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3224..4249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4321..4354)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4824..4840)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4939..4956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5110..5698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5190..5209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5820..6611,6612..6680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6443..6462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6681..6772)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6814..6833
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6839..6858
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    7065..7098
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7411..12658
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggcccgca ctaagcagac tgcccgtaag tgcaacctca ctgtcctttc ccctcgtgtc
     1201 gttcgaccgc gtcatggtcg cgacacccgt gtctcacatg aggaacatca tcactaacta
     1261 tcatctcaat ataggtaagt ccactggtgg caaggctccc cgtaagcagc tcgcctccaa
     1321 ggctgcccgt aaggccgctc cctccaccgg aggtgtcaag aagcctcacc gttacaagcc
     1381 cggtatgtat acactcatca ttcaaagtgg caaatggcct atccgaacag tcaactaaca
     1441 gtctaccaaa ggtaccgtcg ctctgcgtga aatccgtcgt taccagaaga gcactgagct
     1501 cctgatccgc aagctgccct tccagcgtct tgtccgtgaa attgctcagg acttcaagtc
     1561 ggatctccgc ttccagtcct ccgccatcgg tgctcttcag gagtccgtcg aggcctacct
     1621 cgtctccctc ttcgaggaca ccaacctgtg cgccatccac gccaagcgtg tcaccatcca
     1681 gtccaaggac atccagcttg cccgccgtct ccgtggtgag cgctctgcct cctccgagga
     1741 cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
     1801 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1861 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca
     1921 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     1981 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     2041 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     2101 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     2161 caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa
     2221 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc
     2281 cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga
     2341 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta
     2401 agcggacatt cgatttatgc cgttatgact tccttaaaaa agcctttacg aatgaaagaa
     2461 atggaattag acttgttatg tagttgattc tacaatggat tatgattcct gaacttcaaa
     2521 tccgctgttc attattaatc tcagctcttc ccgtaaagcc aatgttgaaa ctattcgtaa
     2581 atgtacctcg ttttgcgtgt accttgctta tcacgtgata ttacatgacc tggacagagt
     2641 tctgcgcgaa agtcataacg taaatcccgg gcggtaggtg cgtcccgggc ggaaggtagt
     2701 tttctcgtcc accccaacgc gtttatcaac ctcaactttc aacaaccatc atgccaccaa
     2761 aagcgcgtaa aacaaagcga gatttgattg agcaagaggg caggatccaa tgcgcgattc
     2821 aagacattaa aaatggaaaa tttcaaaaaa ttgcgcccgc agcgcgtgca tacaaaattc
     2881 atcccaatac aagtgtaatg ctagtggagg tcaacacatc aatgcctatt ttggtttagt
     2941 cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag atggttcatt taggcaactg
     3001 gtcagatcag ccccacttgt agcagtagcg gcggcgctcg aagtgtgact cttattagca
     3061 gacaggaacg aggacattat tatcatctgc tgcttggtgc acgataactt ggtgcgtttg
     3121 tcaagcaagg taagtggacg acccggtcat accttcttaa gttcgccctt cctcccttta
     3181 tttcagattc aatctgactt acctattcta cccaagcatc caaatgaaaa agcctgaact
     3241 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     3301 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     3361 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     3421 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag
     3481 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     3541 cgaactgccc gctgttctcc agccggtcgc ggaggccatg gatgcgattg ctgcggccga
     3601 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     3661 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     3721 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     3781 ggactgcccc gaagtccggc acctcgtgca tgcggatttc ggctccaaca atgtcctgac
     3841 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     3901 atacgaggtc gccaacatcc tcttctggag gccgtggttg gcttgtatgg agcagcagac
     3961 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcgcctcc gggcgtatat
     4021 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     4081 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     4141 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4201 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg
     4261 accgatcccc actaccgcat taagacctca gcgcggccgc aaatttaaat aaaatgaagt
     4321 gaagttccta tactttctag agaataggaa cttctatagt gagtcgaata agggcgacac
     4381 aaaatttatt ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt
     4441 gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc
     4501 gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat
     4561 aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta
     4621 tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa
     4681 agtgacaggc gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa
     4741 aaacccgccg aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac
     4801 cgcccagggg gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag
     4861 aaacgcaaaa aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct
     4921 actctcgcct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5521 gtggtgggct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca
     5761 cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct
     5821 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5881 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5941 agcgctgcga tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac
     6001 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6061 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6121 gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6181 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6241 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6301 atggttatgg cagcgctaca taattctctt actgtcatgc catccgtaag atgcttttct
     6361 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6421 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6481 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6541 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6601 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6661 cggaaatgtt gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt
     6721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc
     6781 agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat
     6841 ggctcataac accccttgtt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     6901 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc cccatgcgag
     6961 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     7021 gcccgggcta attatggggt gtcgccctta ttcgactcta tagtgaagtt cctattctct
     7081 agaaagtata ggaacttctg aagtggggat ttaaatgcgg ccgcgctgag ggtttaatcg
     7141 acgaagcagc tgacggccag tgccaagctt aacgcgtacc gggcccagta tatgttccgc
     7201 agatgactgg agctctgcca tacgtgccct ctcaagcacc atttgttcca tctacagaga
     7261 ctagtcacca actagtctat caagactcac agggtacatt gctgagacca actgaccaga
     7321 ggcagggtag cggattgacg gctccatctc cttcacttac aaggtctatt gaaagccctt
     7381 tagcatcacc aagcggagaa tagattgtta agcttatttt ttgtatactg ttttgtgata
     7441 gcacgaagtt tttccacggt atcttgttaa aaatatatat ttgtggcggg cttacctaca
     7501 tcaaattaat aagagactaa ttataaacta aacacacaag caagctactt tagggtaaaa
     7561 gtttataaat gcttttgacg tataaacgtt gcttgtattt attattacaa ttaaaggtgg
     7621 atagaaaacc tagagactag ttagaaacta atctcaggtt tgcgttaaac taaatcagag
     7681 cccgagaggt taacagaacc tagaagggga ctagatatcc gggtagggaa acaaaaaaaa
     7741 aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
     7801 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
     7861 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
     7921 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
     7981 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
     8041 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
     8101 tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac
     8161 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
     8221 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
     8281 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
     8341 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
     8401 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
     8461 gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt
     8521 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc
     8581 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
     8641 cggggctgaa tgtaacttgc ccttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
     8701 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
     8761 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
     8821 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
     8881 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
     8941 gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct
     9001 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta
     9061 cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat
     9121 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
     9181 atccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
     9241 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
     9301 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
     9361 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
     9421 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
     9481 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
     9541 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
     9601 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg
     9661 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg
     9721 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
     9781 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
     9841 gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac
     9901 aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca
     9961 gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg
    10021 ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc
    10081 caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga
    10141 gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc
    10201 aatggaaacg gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc
    10261 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
    10321 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
    10381 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
    10441 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
    10501 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
    10561 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
    10621 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
    10681 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
    10741 tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
    10801 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
    10861 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
    10921 aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac
    10981 ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg
    11041 ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc
    11101 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
    11161 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
    11221 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
    11281 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
    11341 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
    11401 aggaaaggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
    11461 ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa
    11521 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta
    11581 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
    11641 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
    11701 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
    11761 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
    11821 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
    11881 aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt
    11941 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
    12001 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
    12061 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
    12121 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
    12181 agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc
    12241 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
    12301 tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc
    12361 cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta
    12421 gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa
    12481 cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta
    12541 gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt
    12601 aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagct
//
