LOCUS       sequence-483958-        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human PHF13 with aa 24-40 and 232-238 deletion fused 
            to mEYFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13 ∆24-40∆232-238
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..318
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     255..276
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggcaacatt ctgggggcac aagctggagt acaattccaa cagccacaac gtcttatatc
       61 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      121 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      181 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
      241 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      301 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagctttcat ggactctgac
      361 tcttgcgccg ccgccttcca cccggaggaa tactccccca gttgcaagag gcgcaggtac
      421 atcccttatc cgaaggagga actcccttta aggagcagcc ccagccctgc taacagcact
      481 gctggtacca ttgacagcga cggctgggac gcgggtttct cagacatcgc gtcctcagtg
      541 cccttgccag tctctgaccg ctgctttagc cacctgcagc ctactctctt gcagcgagcc
      601 aagcccagta acttcctgct ggacagaaag aaaacggaca agctgaagaa gaagaagaag
      661 aggaagcgca gggacagtga tgcgcctggg aaagaggggt acaggggggg cttgctgaag
      721 ctggaagccg ctgaccccta cgtggagacc cccacgagtc ccaccttgca ggatatcccc
      781 caggctccca gcgacccctg ctcgggctgg gactccgata ctccctcgag tggatcttgt
      841 gccactgtgt cacctgatca ggtcaaagaa ataaaaactg aaggcaaacg gactatcgtc
      901 cggcagggaa agcaggtggt gttccgagat gaggacagca ctggcaatga tgaggacatc
      961 atggtggact cagatgacga ttcctgggac aagccatttg ccggccgccc catgatcgag
     1021 tgtaatgagt gccacacctg gattcacctg tcctgtgcga aaatccggaa atccaatgtt
     1081 ccagaagtgt ttgtctgcca aaagtgccgg gactccaagt ttgacatccg ccgttccaac
     1141 cgctcgcgga cgggctcccg gaagctgttc ctggactgac cggatccacc ggatctagat
     1201 aactgatcat tattcagcct accttcc
//
