LOCUS       sequence-483984-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human PHF13 with aa 24-40 deletion and N terminal tet 
            operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13∆24-40
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     65..89
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctttaagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
       61 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      121 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg
      181 gtaccgagct cggatccatg gactctgact cttgcgccgc cgccttccac ccggaggaat
      241 actcccccag ttgcaagagg cgcaggtaca tcccttatcc gaaggaggaa ctccctttaa
      301 ggagcagccc cagccctgct aacagcactg ctggtaccat tgacagcgac ggctgggacg
      361 cgggtttctc agacatcgcg tcctcagtgc ccttgccagt ctctgaccgc tgctttagcc
      421 acctgcagcc tactctcttg cagcgagcca agcccagtaa cttcctgctg gacagaaaga
      481 aaacggacaa gctgaagaag aagaagaaga ggaagcgcag ggacagtgat gcgcctggga
      541 aagaggggta cagggggggc ttgctgaagc tggaagccgc tgacccctac gtggagaccc
      601 ccacgagtcc caccttgcag gatatccccc aggctcccag cgacccctgc tcgggctggg
      661 actccgatac tccctcgagt ggatcttgtg ccactgtgtc acctgatcag gtcaaagaaa
      721 taaaaactga aggcaaacgg actatcgtcc ggcagggaaa gcaggtggtg ttccgagatg
      781 aggacagcac tggcaatgat gaggacatca tggtggactc agatgacgat tcctgggacc
      841 tcgtgacctg cttctgcatg aagccatttg ccggccgccc catgatcgag tgtaatgagt
      901 gccacacctg gattcacctg tcctgtgcga aaatccggaa atccaatgtt ccagaagtgt
      961 ttgtctgcca aaagtgccgg gactccaagt ttgacatccg ccgttccaac cgctcgcgga
     1021 cgggctcccg gaagtgttcc cgggga
//
