LOCUS       sequence-484391-        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IVT template for making modRNA encoding PuroR-P2A-iCre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: pIVT-PuroR-P2A-iCre-polyA_pKG03717
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..597
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1..20)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     497..517
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             607..663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             670..690
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
       61 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
      121 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
      181 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
      241 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
      301 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
      361 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
      421 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
      481 gagacatccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
      541 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgga
      601 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
      661 cctatggtgc ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca ccaaaacctg
      721 cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc
      781 agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc
      841 tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg
      901 agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac
      961 ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat
     1021 gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc
     1081 aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac
     1141 tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg
     1201 ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg
     1261 agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag
     1321 gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct
     1381 gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct
     1441 gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg
     1501 atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc
     1561 agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga aatcatgcag
     1621 gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct ggactctgag
     1681 actggggcca tggtgaggct gctcgaggat ggggac
//
