LOCUS       sequence-484967-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 in 
            bacterial cells for calcium-induced NeissLock protein ligation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Lim SYT, Keeble AH, Howarth MR
  TITLE     Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for 
            Anhydride-Mediated Cell Ligation.
  JOURNAL   ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515.
  PUBMED    40947984
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515."
COMMENT     Sequence Label: AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..54
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             64..81
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             532..579
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
ORIGIN
        1 atgggcagcg gcctgaacga tatttttgaa gcgcagaaaa ttgaatggca tgaaggcagc
       61 agccatcatc atcatcatca cttctactcg gacgatcgtt tgaatgtgac cgaagagttg
      121 acgtccaacg acaaaacacg tatcttgaat gtccagtccc gtctgactga cgccaaacgt
      181 attaattggc gcactgtact tagcggtggc agtctttaca tcgaaatccc agggggcgcc
      241 ttgcctgaag gctctaaaga ttcgtttgcc gtgttgttgg agtttgccga ggagcagtta
      301 cgcgcagatc atgtgttcat cgccttccac aagaaccgcg aagatcgtgc agcccttctg
      361 cgtactttct ccttccttgg cttcgagatc gtgcgtccag ggcatccgct ggtgcccaag
      421 cgtcctgacg cttgttttat ggcatatact ttcgaaggcg tatatgatcc gctggcgttg
      481 gacctggatg gtgacggaat cgagacggtg gctgccaaag gagggagcgg tcgaggtgtt
      541 ccacatattg ttatggttga tgcttacaag cgatacaagt aa
//
