LOCUS       sequence-485174-        4117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The pEdit series plasmids are derived from the pTarget series 
            plasmids by inserting homologous recombination arms targeting the 
            gene of interest, which enables gene knockout or gene insertion at 
            the t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Gou L, Liu D, Fan TP, Deng H, Cai Y
  TITLE     Construction of a CRISPR-Cas9-Based Genetic Editing Tool for 
            Serratia marcescens Using a Stationary Phase Promoter and Its 
            Application in Putrescine Production.
  JOURNAL   Biotechnol Bioeng. 2025 May;122(5):1233-1244. doi: 
            10.1002/bit.28949. Epub 2025 Feb 5.
  PUBMED    39910976
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.28949";
            journalName: "Biotechnol Bioeng"; date: "2025-05"; volume: "122"; 
            issue: "5"; pages: "1233-1244"
COMMENT     Sequence Label: pEdit
FEATURES             Location/Qualifiers
     source          1..4117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(984..1059)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1080..1114)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(1191..1208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1362..1950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1442..1461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2124..2915)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cggcgagacg cggcctgaat cagacagggc ggccgccgag ggaacattga tgatagaagc
       61 ggtgagcgtg gagggaaatg cctgaaaatg tcgaaatctt gcctgtttct gttcgattgc
      121 ggggaaacgg ggcagcgtgc ggaccgcctg aacgacggcc tgcacgccgg cgacattact
      181 ggttcaacag cggtttcagg aagcgtgcgg tgtgcgattt ctcgcagtcg gcgaccgttt
      241 ccggcgtgcc ggcgaccagg atctcgccgc cgccgctgcc gccttccggc cccagatcga
      301 cgatccagtc ggcggttttg atcacgtcca ggttatgttc gatgaccacg atggtgttgc
      361 cctgatcgcg cagctgatgc agcaccgcca gcagctgttg gatatcggcg aagtgcagac
      421 cggtggtcgg ttcgtcgagg atatacagcg tttggccggt gccgcgcttc gacagttcgc
      481 gcgccagctt gacgcgctgc gcttcaccgc cggagagcgt ggtggccgac tggccgaggc
      541 ggatgtagga caggccgaca tccatcaggg tctgcagctt gcgcgccagc gccggcaccg
      601 cgtcgaagaa atcgcgcgct tcttcgatgg tcatctccag cacttcgtgg atgctcttgc
      661 ccttgtactt cacttccagc gtttcgcggt tataacgctt gcccttgcac tggtcgcacg
      721 gcacgtagat gtccggcagg aagtgcatct cgaccttgat caccccgtcg ccctggcagg
      781 cttcacagcg cccgcccttg acgttgaagc tgaagcggcc cggcgtgtaa ccgcggctgc
      841 gcgattccgg cacgccggcg aacagctcgc gcaccggggt gaagataccg gtgtaggtgg
      901 ccgggttgga gcgcggtgtg cggccgatcg ggctctgatc gatgtcaagc ttctgcaggt
      961 cgactctaga gaattcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     1021 actagcctta ttttaacttg ctatttctag ctctaaaacc cgggtaaatc gtattgctca
     1081 ctagtattat acctaggact gagctagctg tcaaggatcc agcatatgcg gtgtgaaata
     1141 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     1201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1801 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2041 atcttcacct agatccttta cgcgttcgcg gaacttcgga ataggaactt atgagctcag
     2101 ccaatcgact ggcgagcggc atcttatttg ccgactacct tggtgatctc gcctttcacg
     2161 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
     2221 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
     2281 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
     2341 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
     2401 agcgttaagg tctcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
     2461 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     2521 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
     2581 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     2641 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
     2701 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     2761 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     2821 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     2881 tgagtcgata cttcggcgat caccgcttcc ctcatgacat tgcactccac cgctgatgac
     2941 atcagtcgat catagcacga tcaacggcac tgttgcaaat agtcggtggt gataaactta
     3001 tcatcccctt ttgcttatgg agctgcacat gaactcgagt agggataaca gggtaataga
     3061 tctgcaaaca atggctgggt gagttgctgg gccgctatca gggcggcacc atcgagccga
     3121 ccttccgctg cgactacggc tataacatct atctcggcaa gaacttctac gccaacttcg
     3181 attgcgtgat cctcgacgta tgcgaggtgc atatcggcga caactgcctg ctggcgccgg
     3241 gcgtgcatat ctataccgcc acccatccgc tggacgccga gactcgcgtc ggcggggcgg
     3301 agttcggtaa gagtgtcagg attggcgaca acgtgtggat cggcgggcgc gcggtgatca
     3361 atccgggagt gaccatcggc gataatgcgg tcgtggcctc cggcgcggtg gtgaccaaag
     3421 acgtgccggc caattgcgtg gtgggcggca acccggccag gatcatcaag cagctataaa
     3481 aaaacgggcg ccgccgggcg cccgccgtca tcaggcgcgc agcgagtcga tatccaccac
     3541 gaagcggtac ttcacgtcgc ttttcagcat gcgttcatag gcctcattaa tttgctgcat
     3601 cgggatcagc tcgatatccg acgtgatgcc gtgctgcccg cagaagtcca gcatctcttg
     3661 cgtttccgcg atgccgccga tcagcgaacc ggcgatgcgg cggcgcttca tgatcaggtt
     3721 gaacacctgc ggtgacggat gatcgtgctc cggcgcgccg accagcgtca gggtgccgtc
     3781 gcggcgcagc aagttgagga acgggttcag atcgtgctga gccgctaccg tgttgaggat
     3841 gaagtcgaag ctgttgacgt gctgcgccat ctcatccggg ttgcgcgaaa tcaccacttc
     3901 atgggcgccg aggcgcttgg cgtcttcgat tttcgacggc gaggtggtga acagcaccac
     3961 atgcgcgccc atcgcccgcg ccagtttgac gcccatgtgc cccagcccgc cgagcccgac
     4021 gatgccgacc tttttgcctg gccccgcgcc ccactggcgc agcggtcgct ccagttatca
     4081 atgtaggggg gacaacgttt gaccggcagc gaagtgc
//
