LOCUS       sequence-485236-        8930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing pad vector with two Bxb1 recombination sites for RMCE and 
            LNGFR + EGFP-HygroR for sorting/selection and 2x inducible 
            expression cassette for shRNA resistant chicken H2B..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8930)
  TITLE     A novel approach towards a histone replacement system in Tetrapods
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHREP-LP-2xH2B
FEATURES             Location/Qualifiers
     source          1..8930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        341..720
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        721..924
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     875..895
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     969..988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        969..987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1007..1054
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     regulatory      1058..1067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1886..1939
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1964..1966,1967..1969,1970..2680)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1964..1966,1967..1969,1970..2680)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2009..2030)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2270..2289)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2617..2638
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2699..3724
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2699..3724
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3854..3975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4050..4362
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4058..4076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4094..4112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4129..4147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4165..4183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4201..4219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4236..4254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4272..4290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4825..4842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4843..4890
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    4929..5050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             5113..5130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5131..5178
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    5217..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5429..5741
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    5437..5455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5473..5491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5508..5526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5544..5562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5580..5598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5615..5633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5651..5669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6204..6221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6222..6269
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    6308..6429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6495..6542
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(6927..6944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7098..7686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7178..7197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7857..8648,8649..8717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8480..8499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8718..8822)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agaccgttac
      301 ataacttaca atcaggggat aacgcaggaa agaaaccggt gacattgatt attgactagt
      361 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      421 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      481 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      541 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      601 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      661 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      721 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      781 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      841 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      901 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      961 atcgaaatta atacgactca ctatagggag acccaagctg gctagcggtt tgtctggtca
     1021 accaccgcgg tctcagtggt gtacggtaca aaccggcgcc gccatggggg caggtgccac
     1081 cggccgcgcc atggacgggc cgcgcctgct gctgttgctg cttctggggg tgtcccttgg
     1141 aggtgccaag gaggcatgcc ccacaggcct gtacacacac agcggtgagt gctgcaaagc
     1201 ctgcaacctg ggcgagggtg tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc
     1261 ctgcctggac agcgtgacgt tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg
     1321 caccgagtgc gtggggctcc agagcatgtc ggcgccgtgc gtggaggccg acgacgccgt
     1381 gtgccgctgc gcctacggct actaccagga tgagacgact gggcgctgcg aggcgtgccg
     1441 cgtgtgcgag gcgggctcgg gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg
     1501 cgaggagtgc cccgacggca cgtattccga cgaggccaac cacgtggacc cgtgcctgcc
     1561 ctgcaccgtg tgcgaggaca ccgagcgcca gctccgcgag tgcacacgct gggccgacgc
     1621 cgagtgcgag gagatccctg gccgttggat tacacggtcc acacccccag agggctcgga
     1681 cagcacagcc cccagcaccc aggagcctga ggcacctcca gaacaagacc tcatagccag
     1741 cacggtggca ggtgtggtga ccacagtgat gggcagctcc cagcccgtgg tgacccgagg
     1801 caccaccgac aacctcatcc ctgtctattg ctccatcctg gctgctgtgg ttgtgggtct
     1861 tgtggcctac atagccttca agagggaggg caggggaagt ctcctaacat gcggggacgt
     1921 ggaggaaaat cccggcccct cccgggagat gggggaggct aacatggtga gcaagggcga
     1981 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2041 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2101 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2161 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2221 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2281 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2341 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2401 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2461 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2521 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2581 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2641 cgccgccggg atcactcttg gcatggacga gctgtacaaa accggtgatc agcacgtgat
     2701 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     2761 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     2821 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     2881 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     2941 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     3001 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     3061 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     3121 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     3181 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     3241 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     3301 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     3361 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     3421 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     3481 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     3541 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     3601 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     3661 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     3721 atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3781 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3841 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3961 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcggcgtc
     4021 agtttcggta aggggtccgc tatctagacg agtttactcc ctatcagtga tagagaacgt
     4081 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     4141 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     4201 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     4261 gtcgagttta ctccctatca gtgatagaga acgtatgtcg attaggcgtg tacggtgggc
     4321 gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcca attccaactc
     4381 ttttgcctta tacctacttt ccgtaccact tcctaccctc gaaaggcctc tgaggccacc
     4441 atgccagagc ccgcaaagtc cgcacccgcc ccgaagaagg gctccaagaa ggcggtcacc
     4501 aagacccaga agaagggcga caagaagcgc aagaagagcc gcaaggagag ctactcgata
     4561 tatgtctata aagtcctcaa acaggtgcac cccgacacgg gcatcagcag aaaagcaatg
     4621 ggtataatga atagctttgt caatgacatc ttcgagcgca tcgcgggcga ggcgtcgcgc
     4681 ctggcgcact acaacaagcg cagcaccatc acgtcgcggg agatccagac cgccgtgcgg
     4741 ctgctgctgc ccggcgagct ggcgaagcac gcggtctccg agggcacgaa ggcggtcacc
     4801 aagtacacca gctccaagaa gcttcaccat caccatcacc atcgtgacca catggtcctt
     4861 catgagtatg taaatgctgc tgggattaca tagggcctgt caggccaagc ttccatcgat
     4921 agacatgata agatacattg atgagtttgg acaaaccaca acaagaatgc agtgaaaaaa
     4981 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5041 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     5101 ggaggtttgc ttcaccatca ccatcaccat cgtgaccaca tggtccttca tgagtatgta
     5161 aatgctgctg ggattacata gggcctgtca ggccaagctt ccatcgatag acatgataag
     5221 atacattgat gagtttggac aaaccacaac aagaatgcag tgaaaaaaat gctttatttg
     5281 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5341 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttattc
     5401 gtttcggtaa ggggtccgct atctagacga gtttactccc tatcagtgat agagaacgta
     5461 tgtcgagttt actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga
     5521 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     5581 ccctatcagt gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg
     5641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga ttaggcgtgt acggtgggcg
     5701 cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagccaa ttccaactct
     5761 tttgccttat acctactttc cgtaccactt cctaccctcg aaaggcctct gaggccacca
     5821 tgccagagcc cgcaaagtcc gcacccgccc cgaagaaggg ctccaagaag gcggtcacca
     5881 agacccagaa gaagggcgac aagaagcgca agaagagccg caaggagagc tactcgatat
     5941 atgtctataa agtcctcaaa caggtgcacc ccgacacggg catcagcaga aaagcaatgg
     6001 gtataatgaa tagctttgtc aatgacatct tcgagcgcat cgcgggcgag gcgtcgcgcc
     6061 tggcgcacta caacaagcgc agcaccatca cgtcgcggga gatccagacc gccgtgcggc
     6121 tgctgctgcc cggcgagctg gcgaagcacg cggtctccga gggcacgaag gcggtcacca
     6181 agtacaccag ctccaagaag cttcaccatc accatcacca tcgtgaccac atggtccttc
     6241 atgagtatgt aaatgctgct gggattacat agggcctgtc aggccaagct tccatcgata
     6301 gacatgataa gatacattga tgagtttgga caaaccacaa caagaatgca gtgaaaaaaa
     6361 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6421 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     6481 gaggtttacg tagaggtttg tctggtcaac caccgcggac tcagtggtgt acggtacaaa
     6541 ccacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaagtttt tgtgtctctc
     6601 acttctgagg cggaaagaac cagctgggcc tactagctac tcgggacccc ttaccgaaac
     6661 atcgccgcat tctgcagagg agtcgagtgt atgtaaactt ctgacccact gggaatgtga
     6721 tgaaagaaat aaaagctgaa atgaatcatt ctctctacta ttattctgat atttcacatt
     6781 cttaaaataa agtggtgatc ctaactgacc taagacaggg aatttttact aggattaaat
     6841 gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg taaacttccg
     6901 acttcaactg tatagggatc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8821 cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag
     8881 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
//
