LOCUS       sequence-485982-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor vector for targeting a tdTomato reporter to the human SFTPC 
            locus at the endogenous ATG start site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, 
            Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias 
            W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, 
            Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers 
            MF, Kotton DN
  TITLE     Differentiation of Human Pluripotent Stem Cells into Functional Lung
            Alveolar Epithelial Cells.
  JOURNAL   Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 
            10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.
  PUBMED    28965766
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.08.014"; journalName: "Cell Stem Cell"; date: 
            "2017-10-5- 5"; volume: "21"; issue: "4"; pages: "472-488.e10"
COMMENT     Sequence Label: p1303 DV-SFTPC-tdTomato
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(32..50)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(71..87)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(84..106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(119..125,126..143,144..149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(302..319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(473..1061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(553..572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1232..2023,2024..2092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1855..1874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2093..2197)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2223..2678)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2360..2381)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2572..2591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2805..2827
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2819..2836
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2820..2836
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2843..2862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2843..2861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2881..2897
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4689..6119
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     intron          6170..6399
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6382..6401
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(6445..6462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6451..6675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6720..6736
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6771..6804)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(7347..7946)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7430..7450)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7927..7946
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7958..8457)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8006..8025)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8399..8422
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(8491..8524)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8538..8554)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8538..8554)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
       61 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1081 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1141 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1201 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1261 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1321 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1381 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1441 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1501 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1561 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1621 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1681 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1741 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1801 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1861 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1921 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1981 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2041 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2101 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2161 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2221 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2281 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2341 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2401 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2461 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2521 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2581 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2641 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     2701 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2761 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     2821 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccctcgat
     2881 cgaggtcgac ggtatcgata agcttctgat ggaattagaa cttggcaaaa caatactgag
     2941 aatgaagtgt atgtggaaca gaggctgctg atctcgttct tcaggctatg aaactgacac
     3001 atttggaaac cacagtactt agaaccacaa agtgggaatc aagagaaaaa caatgatccc
     3061 acgagagatc tatagatcta tagatcatga gtgggaggaa tgagctggcc cttaatttgg
     3121 ttttgcttgt ttaaattatg atatccaact atgaaacatt atcataaagc aatagtaaag
     3181 agccttcagt aaagagcagg catttatcta atcccacccc acccccaccc ccgtagctcc
     3241 aatccttcca ttcaaaatgt aggtactctg ttctcaccct tcttaacaaa gtatgacagg
     3301 aaaaacttcc attttagtgg acatctttat tgtttaatag atcatcaatt tctgcaggtc
     3361 gactctagct agaggatccc caaatctctg taggtagttt gtccaattat gtcacaccac
     3421 agaagtaagg ttccttcaca aagagatcgc ctgacacgat ttcctgcaca ggcttgagcc
     3481 atatactcat acatcgcatc ttggccacgt tttccacggg tttcaaaatt aatctcaagt
     3541 tctacgctta acgctttcgc ctgttcccag ttattaatat attcaacgct agaactcccc
     3601 tcagcgaagg gaaggctgag cactacacgc gaagcaccat caccgaacct tttgataaac
     3661 tcttccgttc cgacttgctc catcaacggt tcagtgagac ttaaacctaa ctctttctta
     3721 atagtttcgg cattatccac ttttagtgcg agaaccttcg tcagtcctgg atacgtcact
     3781 ttgaccacgc ctccagcttt tccagagagc gggttttcat tatctacaga gtatcccgca
     3841 gcgtcgtatt tattgtcggt actataaaac cctttccaat catcgtcata atttccttgt
     3901 gtaccagatt ttggcttttg taaattccag cctgcaattg acagacaagc ccagagcaaa
     3961 gaaggaagca ctgcacatga gtaagagctt gccaccagtg gggacagagt ttccagaatt
     4021 aggaaaataa tcactggggg caagtttgag gttggtacca gatatgtggg aggaggcaag
     4081 gtaagggaaa gagtacttga agttggaact ggtccttgca gggaaatgca catttatgaa
     4141 accccgaaaa ctgatgtcaa agcacctcct gccttgggca gagtcctctc agagtctaca
     4201 ggtgctgcct ccagaaccct cttcctggag cgcatcccta tgtatctaga aattctgctg
     4261 ggaaatatga tggtcagacc cttggccacc tgaaaggttc agggtggtag aagaaaaagg
     4321 aaagccacag ggcagcaggg gcaggtgcca gcaaggaagg caggcacgcc aggaagacac
     4381 ccatggtgag aagtgcagat ggcccgaggg caagtttgct caactcaccc aggtttgctc
     4441 ttgctggggc caagaggact catgtgccag ggccaagggc ccttgggggc tctcacaggg
     4501 ggcttatctg ggcttcggtt ctggagggcc aggaacaaac aggcttcaaa gccaagggct
     4561 tggctggcac acagggggct tggtccttca cctctgtccc ctctccctac ggacacatat
     4621 aagaccctgg tcacacctgg gagaggagga gaggagagca tagcacctgc agcaagacgc
     4681 gtggtaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     4741 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4801 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     4861 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     4921 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4981 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     5041 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     5101 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     5161 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     5221 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     5281 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     5341 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     5401 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     5461 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     5521 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     5581 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     5641 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     5701 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     5761 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     5821 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     5881 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     5941 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     6001 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6061 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaac
     6121 atatgtctag atggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc
     6181 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat
     6241 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa
     6301 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact
     6361 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac
     6421 tgcaggtcgg agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6601 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6661 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgatc ctctagactc
     6721 gaggtcgacg gtatcgataa gctagcttgg gctgcaggtc gagggaccta ataacttcgt
     6781 atagcataca ttatacgaag ttatattaag ggttccggat cagcttgata tcgaattagc
     6841 ttctgatgga attagaactt ggcaaaacaa tactgagaat gaagtgtatg tggaacagag
     6901 gctgctgatc tcgttcttca ggctatgaaa ctgacacatt tggaaaccac agtacttaga
     6961 accacaaagt gggaatcaag agaaaaacaa tgatcccacg agagatctat agatctatag
     7021 atcatgagtg ggaggaatga gctggccctt aatttggttt tgcttgttta aattatgata
     7081 tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa gagcaggcat
     7141 ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc aaaatgtagg
     7201 tactctgttc tcacccttct taacaaagta tgacaggaaa aacttccatt ttagtggaca
     7261 tctttattgt ttaatagatc atcaatttcc gatgcatggg gtcgtgcgct cctttcggtc
     7321 gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg
     7381 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     7441 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     7501 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     7561 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     7621 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     7681 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     7741 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     7801 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     7861 tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg
     7921 cgcaccgtgg gcttgtactc ggtcatggta agctggtcga aaggcccgga gatgaggaag
     7981 aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg
     8041 accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc
     8101 ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa
     8161 ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg
     8221 ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga
     8281 ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc
     8341 taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca
     8401 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
     8461 agaattaatt cctgcaggtc gagggaccta ataacttcgt atagcataca ttatacgaag
     8521 ttatattaag ggttccggat ccactagttc tagactcgag tatggcgcgc cgatgtgggc
     8581 agcaaagagg tcctgatgga gagcccgccg gtgagtgtgg ttgcgtgtgt gtatgtatgt
     8641 gtgcgcgcgc acatgtgtgt gatgggccct gcctcctcta tcctccctgg cctgtttcct
     8701 tatccagatc cattcactca actaacctag gactgtgata agtcaggatg gggacaccaa
     8761 gaccactaag ccagggaccc ttggggagct gtttgtgggc caagagccac tataggggtc
     8821 cgtagaaagg gctgtccgta gacagccctg agtcagaagc catgagaaac ttcagaagtc
     8881 aggggacact tctcagagaa aaaccacata cgagctggag ccagaataag gaggagctcg
     8941 cccggtggag aaggaggaag gcattccagg aaggagggag actctgtatc accgcatgga
     9001 ggtgatcact tggggagaga gaggggctga ccatggctgg gggaagcagc agggagagac
     9061 aggtgaagca ggctctcttg ggtccctcaa aactagaccc tgcttctaag cttctatgtg
     9121 tctatgggtt tgttagaatc caggccacct cctccaagaa gccttctctg atctcctcag
     9181 cccttccctg tccatccatc gcatcggctg tccagcccta ggcagccgtg ggagggtgtt
     9241 cagcttgtat agggagaaga ggggacagcc tcatgacctc atgcctgtct ccctgcctgc
     9301 cccaccgtgt gactactccg gcggc
//
