LOCUS       sequence-486046-       18818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor vector I, accepting Donor I first, with attPTT-FRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18818)
  AUTHORS   Zhao C, Stepanova AN, Alonso JM
  TITLE     DASH: a versatile and high-capacity gene stacking system for plant 
            synthetic biology.
  JOURNAL   Plant Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179.
  PUBMED    40493021
REFERENCE   2  (bases 1 to 18818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179."
COMMENT     Sequence Label: pDASH-AIK
FEATURES             Location/Qualifiers
     source          1..18818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    451..475
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(585..618)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    708..732
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9680..10498)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10406..10425
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10490..10509
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    16923..16944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16959..16964,16965..16982,16983..16989)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16997..17013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17002..17024
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17021..17037
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17021..17037
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     18651..18669
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgcttcctg tcggccctca ttcgtgcgct ctaggatcga cgatcgtcaa cgttcacttc
       61 taaagaaata gcgccactca gcttcctcag cggctttatc cagcgatttc ctattatgtc
      121 ggcatagttc tcaagatcga cagcctgtca cggttaagcg agaaatgaat aagaaggctg
      181 ataattcgga tctctgcgag ggagatgata tttgatcaca ggcagcaacg ctctgtcatc
      241 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
      301 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
      361 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
      421 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
      481 gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa ttctagtagt
      541 gccccaactg gggtaacctt tgagttctct cagttggggg cgtagaagtt cctatacttt
      601 ctagagaata ggaacttcga attcgtaggg ataacagggt aattccctta attctccgct
      661 catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
      721 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      781 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
      841 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc
      901 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg
      961 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa
     1021 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg
     1081 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc
     1141 tggatctcgc cttcaatccg ggcctgtggc gggaactcca cgaaaatatc cgaacgcagc
     1201 aagatcgtcg atcctccggc gttcagcctg tgccacagcc gacaggatgg tgaccgaatc
     1261 ctacaaggta gaatccgcct gagtcgcaag ggtgacttcg cctatattgg acgacggcgc
     1321 gcagagggcg acctcttttt gggttacgat tgtaggatta tcactaaaac aatacatgaa
     1381 catattcaaa tggcaatctc tctaaggcat tggaaataaa tacaaataac agttgggtgg
     1441 agtttttcga cctgagggcg ttaaccttct gttaacctaa aagctcttgc ccaaacagca
     1501 gaatcggcgc taattgccag cggcggaact tttccagttt cgcgaaaagt atcgccactg
     1561 gcaaggaatg ggtttgagat ggcgaagtct gtcctaaaag cagcgcctgt agttgtaggg
     1621 ttgacggcct tgatggagcg tcatgccgat gccctctcga gccaacttca agcacatcat
     1681 cttaaggttt tcccgccgca ttccgagaag ggcattcgaa cattcgggcc atcggaggcg
     1741 tccaagctgc tcggcgttgg cgagtcatat ttacggcaga ccgcgtctga gatgccagag
     1801 ttgaatgtta gcatgagccc gggtggcagg cgaatgttct caattgaaga tatccatgtg
     1861 attcggaagt atatggatca ggtcggccgc gggaaccggc gctacctgcc acatcgtcga
     1921 ggcggcgagc agcttcaggt tatctctgtg atgaatttca aaggtgggtc gggtaagacc
     1981 accaccgccg cgcatctggc gcagtacctc gctatgcgcg gatatcgagt cttggccatt
     2041 gatctcgatc ctcaagcgag cctttctgca ctctttggga gccaaccgga gacggacgtt
     2101 ggcccgaacg aaacgctcta cggcgctata aggtatgatg atgagcaggt ggcaatcgaa
     2161 cgagtcgtcc gagggactta cattcccgac ctccacctga ttcctggtaa ccttgagctg
     2221 atggagtttg aacacgatac gccacgcgcg ctgatgaacc gcaaagaggg cgacacgctc
     2281 ttttatggtc gcatcagcca agtaattgaa gatatcgcgg ataactatga cgtcgtggtc
     2341 atcgactgcc ctccccagct tgggtatctc acgctatccg cattgactgc ggcgacgtcc
     2401 attcttgtca cggtccatcc gcagatgctg gatgtgatgt cgatgaacca gtttctggca
     2461 atgacatcga accttttgcg tgaaatcgag aatgctggcg ccaagttcaa gtttaattgg
     2521 atgcgctatc tgataacccg tttcgaaccg agcgacggac cacagaacca aatggtaggt
     2581 tatctgcggt cgatttttgg cgaaaatgtc ctcaattttc cgatgcttaa aaccaccgcg
     2641 gtttcggacg ctggcctgac aaaccagact ctattcgaag tggagcgtgg cctgttcacg
     2701 cgctcgacct atgatcgagc cttggaggcg atgaacgccg tcaacgacga gatcgaaaca
     2761 ctgatcaaaa aagcatgggg taggcccaca tgagccggaa gcacatcctt ggcgtctcaa
     2821 ctgacgcccc tgagacgtcg cccgccgaca ataggacggc aaagaaccgc tccatgccgc
     2881 tcctcggcgt aacaaggaag gagcgcgatc cggcaacgaa gctcacagcg aacattggta
     2941 acgcactgcg agagcaaaac gatcgtctta gccgtgccga agagatcgag cggcgtctcg
     3001 ctgaaggtca ggcagtgata gagttggatg cctcgtcaat agaaccgtct ttcgtgcagg
     3061 atcgtatgcg aggggacatt gacgggctcc ttacttcgat ccgggaacaa ggacagcaag
     3121 tcccaatcct tgtgcgaccg catccgagcc agccgggccg atatcaggtt gccttcggcc
     3181 accgccggct acgcgccgtt tcagaactcg gacttccggt cagagcggtc gttcgcgaac
     3241 tgacggacga gcaagtggtc gtagcacagg gtcaggaaaa caatgagcgc gaagatctta
     3301 ccttcatcga aaaggcgcgc ttcgcacatc gcctgaacag gcagttttct cgagagattg
     3361 tcatcgccgc gatgtcgatc gacaagagca atttgtccaa gatgcttctg ctcgtcgacg
     3421 ccctcccctc tgaactgacc gatgctattg gtgccgctcc tggtgttgga cggccgagtt
     3481 ggcaacaact tgccgagctg attgagaaag tttcttcacc ggccgacgtg gctaaatatg
     3541 ctatgtcgga ggaagttcaa gcgctgccat cggcagaacg attcaaggcg gtgatcgcta
     3601 gtctgaagcc cagtcgggtt gcgcgtggac ttcccgaggt catggccacc ccagacggca
     3661 ccagaattgc acaggtgacg cagagcaagg ccaaactgga aatcacgatt gacaggaagg
     3721 cgacgcccga ttttgcgacc ttcgtgctcg atcatgtgcc agcgctgtat caagcgtacc
     3781 acgctgagaa ccaacggaaa cggggagagt aaaccgcaaa agaaaagagc cccctcaacg
     3841 tcgccgtcgc ggaagccctt ctgtctctct agcgcgaaca gaatcgcatt tcctcgaatc
     3901 ctcgtcaaga gtttttagcg ccgttttggt gagctgattt cctttgcctg ctgaaaggtg
     3961 aaagatgatg cagacaggaa gtgtaacgac gccattcggg cggcggccaa tgacgcttgc
     4021 gcttgtgcgg cgccagacgg cgctggccga tatcaaacaa ggcaagacag cggacaagtg
     4081 gaaggtcttt agagacgcgt ccgcggccat ggaactactt ggaatccagt ccaacagtct
     4141 tgccgtcctt gatgcgctat tgagctttca cccggaaacg gagttgcgtc aggaggcaca
     4201 gctgatcgtc ttcccgtcga atgctcagct tgcccttcgg gcgcatggga tggctggcgc
     4261 gactttgcgt aggcacatcg ccatgctcgt ggagtcaggc ttgatcgtcc ggaaggatag
     4321 cgccaacgga aagcgttacg ctcgtaagga tggcgctggt cagatcgagc gcgcgtttgg
     4381 cttcgatttg tctccgcttc tcgcgcggtc cgaagagcta gcgatgatgg cacagcaggt
     4441 gatggccgat cgagcagcat tcaggatggc caaagaaagt ctgacgattt gccgacggga
     4501 cgttcggaag ctaattacgg cagctatgga agagggagcg gagggcgact ggcaagctgt
     4561 cgaggaagtc tatgtggaac ttgtgggtag aattccacgc gccccgacgc ttgctgatgt
     4621 agagtcaatt ctcgaagaga tgtggatgct ccaggaagag ataatcaacc ggttggaaat
     4681 tagagacaat tcagaaaata atagcaccaa tgctgcccag agcgagcagc acatacagaa
     4741 ttcaaaaccc gaatccgtta atgaacttga acctcgctct gaaaaggagc agggcgctaa
     4801 gccgagtgaa atagaccggg cagggagcga gccgataaaa gcgttccccc tcgggatgat
     4861 cctgaaagca tgcccgacca ttggcaatta tgggccgagc ggtgcggttg ctagctggcg
     4921 tgacctcatg tcggctgcgg tggtggttcg gtctatgctg ggggtcagcc cgtcggctta
     4981 ccaagacgcg tgtgaggcaa tgggaccgga gaatgcggca gcagcgatgg cgtgcatttt
     5041 ggagcgagcg aacttcatca attcgcccgg gggctatctc cgagatctga cacggcggag
     5101 cgagctcggg aagttttcac ttggcccgat gataatggcg ctcttgaagg ctagcgggca
     5161 ggggacgttg cggtttggct agaattagcg agtatggagc aggatggtct gtggtcagct
     5221 gaccacagac ctaataggtt gaaaacatga gcgttttttg gatgatcgac agaccatccg
     5281 attcccggag taccaagcgt gctctgatgg gagcgataac attactcaac aagcacgaag
     5341 gccccatgcc gatcgttgat cgtgaaggag agcctgctct acatgcggcg gtattttgcc
     5401 ggccgaggca tgtagtcgcg gagcactgcc tatttactgc cctaggcaca aacgttgact
     5461 cttggatcga gctggcagac aaagcaataa cccacacaga ggacgattaa tggctgacga
     5521 agagatccag aatccgccgg acggtactgc tgctgccgaa gttgagccgg ctgctcctag
     5581 aggtagaaga gcaaagaaag caccagccga aacagcccgc acgggatcgt tcaaatccgt
     5641 gaagccgaaa acccgcggcc tcagcaaccg agaaaaactg gagaagatcg gtcaaatcga
     5701 agctcaggtc gctggcggcg caaccttgaa ggacgccgtt aagatcgtgg gtatttccgt
     5761 tcagacctat tatcaatgga agagagctgc ggttcaacct gtctcacaga atccggccgt
     5821 gtctgtttca gttgacgatg aactcggcga gttcatccaa ctcgaggagg aaaatcggcg
     5881 gctcagaaag ctacagcttc tctctgaaaa attgcgcgca gaaaacgcgg acttacgcaa
     5941 aagacttgga atcaactagc ggcctttggg ggtccctcgc taaatcaact tgacgcgcat
     6001 ctctcaatga atgaatgcct tggaaaattt ccgcgcattc tgatcgttgc gctatcaagc
     6061 aatcccaatc ttctggcata tgaaaaaacc acgacccaaa ttcgtcgtcg aatataagac
     6121 gaacaggcga caagccaagg cccgaccaac gtcaatctgg ggcaatctgg gtttgcaggc
     6181 ggtgggccgt gcggtcgcgg ccgacggagt cgtgccgaag agtagtctgc cgcaagtccg
     6241 ttccgtcctg gagaaggttg tcgctgcaga tccagacgtg gctgccatct ttgctcaggt
     6301 tgagattaag ggccttccgg ctccccccaa tacatcatca ccgaacgagg gtcacatcga
     6361 gccggtagtt attgagggca aggtgtttgc cgcgcctttc gtgcaggagg agcgcacctt
     6421 ggccccgccg caggctccga gatctcgagc gaaaccgcgg acaaaaaggc ggtccccgga
     6481 caccacagaa cgtcctgcag tccaaggtta ccgtcgtggt ccctcggtgc tgccaggttt
     6541 tgaggaagag cttgccgcta tcgaggcgga aaaccgtcgt ctgaagcgcc tcttgatcgt
     6601 gaagctccgt gaggagaacg acaggcttaa gttcgcgctt cgacgatttg ggggcgcctg
     6661 agcacgatgc tggcccactt gcttcagcgg gcaaacaaca tggagtggag atctaatgat
     6721 cgcggcgctt gaacatcgct aggggtcttc ggcgccgctg aaccgtgctc cattttgcac
     6781 tcggagacac agcctcccaa aaacctggcg ccgctcgttg gtgaactcct caacatcgag
     6841 cggatcaagg gaaactggaa tgacatcctt cgacccgtca cggcgatccg ctctggccag
     6901 gtccaaccta cggccatgcc cgcaaagcca acggcatttc cacgtcaagc acattgaccc
     6961 actcggctga cggtacatca gctggcgagc tcatccggac ccagacgaac tgcgttgatc
     7021 tcaggccgtt gcggcagaaa acctccatcc tcgcagccga tccaacactt tcctgtggta
     7081 atgcctattt cagatgaaac agcctttttt aaacttggcg cgtcattctg atattatggc
     7141 cacacctcta cgatctaggg agtgcgagaa gtggaaagaa atgaagaacc cgatttgcac
     7201 acacatacct cgatgcggaa taaaaatgca tcatcgcttg gtgaggccct ggatgacgat
     7261 gcgatcgcca acatcgcgca tcacattgtt acagacgacc cggtggaaac gtcaaaaaac
     7321 ttcacaaaac ttaagctgat taacaaatca gtgaaacagg gtttcgaacg cagtgcgtac
     7381 ggaaaattcc atcgacgcat tagccggctt ggtattgcta gtaaggcgct atatgacaac
     7441 gctgtgccga aggaaggatt cgtagaacat cctgaatatg ctccagctct gcaaagggat
     7501 tatgcgttgg caggagagcg aattgaggcg gttggtggaa ctttcaggtt gcaaacacct
     7561 cagcggaaat cggctcttgt agatcatatc ttgagtatgc ccgaaggttt cgatcaagct
     7621 catgcccttg agtctatagc ccctcatgtt ggcgatctaa gcgcggaaga tcgccatcgt
     7681 gttgtgaaca aaatgctcga gcactttgcc accccgtttc aagaaggggg gctgccaacc
     7741 gacgaaaatt tttgtggaac gtacgctttg tccaaggcgt ttcctcatat ggaagacagc
     7801 ctaaaagcaa agatgctgga tgtggtgtta gagcatcctc acttagccaa cctccattgg
     7861 acgcagagag aactagttga agggtggcat caagaccgtg aagctgagaa gtcagcctgg
     7921 cgttccaatc aaaagccaat tgtcgagggg cgacttgcag acctcgaacg atcgatccac
     7981 agtgatatcg aggtgacaaa caggagtgat tttgcgagga tgcgttcagc caggcccttg
     8041 caacaagcta taagcgatac gttcgatctt gcgcgtgagg agctcaaaag ccgccctcgt
     8101 gaagggcggg gtcgataatt acatctattg gtctttctgc cgaacctcta tgaaatccgc
     8161 acgtcaaatg tggcttttac ttcccgattt caattgaagc gctaagctga aaatcccgtc
     8221 cccatttctg acctaacggt ttcatcaccg gggcagggag cggcgcttaa aacggtgacg
     8281 gccaccacca aaggacatgt tcgctcacgc ggtgcctccg tgcttctttg caagagcctg
     8341 cttcaggctg atgatctcct tcagtgcgtt ggtgtgaatc cgctccaggc gtttcagttc
     8401 gctctccaaa taggcgttgc gcttatccag cctcgtccga tcttcttcca agctctccat
     8461 tttcactagc gcgttatggt aattcaaccg taactcctca aaaaaagcac gttcggcgac
     8521 gaaatgggtt tcggaatcga gttcaggtat gtcgccgaca tgcacgacgc gaaccgcctt
     8581 gccggcagcg gcgagccggg cgcggtaagc gcgttgaatt tccgcttggg tcagcgcgtt
     8641 tggcttgcgg ggccgcgagg aaatttggga gtaaccataa cggttattta tcataacgcg
     8701 ttatcactgg atagcatcgg cgcaggcgga tggcgcgctt cgccgggtcg aaatcaatat
     8761 ttccagatcg agatggattg aagacctgaa gggccctcag agcccagacg ccttggttag
     8821 gttggtgcgg aaccgatccc gccgccgctg gtatttgcgc gtcatctcgg ccgaggcgtg
     8881 gcctagttgt ttctgaacgt aacgctcctc aatgtcggcc gaggaggcaa gaccggcgcg
     8941 cagcgagtgc cctgagaaca gcagcgcccg ttccccttct ggaagatcgg aacggacgcc
     9001 ggcggcgagt gcggtctgct tgaccaggcg ggcgacatgc ttgtcggaga gccgctcgac
     9061 gtccacggtc ttgttgtcct tgaagatgcg ccggaacagg ggaccgcgcg cgatcctgcc
     9121 gaagcggatc gtagtgacca ccatttgccc catatcaccg tcggtactga tcccgtcgtc
     9181 aataaaccga accgctacac cctgagcatc aaactctttt atcagttgga tcatgtcggc
     9241 ggtgtcgcgg ccaagacggt cgagcttctt caccagaatg acatcacctt cctccacctt
     9301 catcctcagc aaatccagcc cttcccgatc tgttgaactg ccggatgcct tgtcggtaaa
     9361 gatgcggtta gcttttaccc ctgcatcttt gagcgctgag gtctgcctcg tgaagaaggt
     9421 gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca
     9481 cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc
     9541 acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt
     9601 cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     9661 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     9721 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     9781 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     9841 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     9901 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcagctta tgcatttctt
     9961 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
    10021 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
    10081 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
    10141 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
    10201 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
    10261 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
    10321 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
    10381 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
    10441 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
    10501 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
    10561 tatcttgtgc aatgtaacat cagagatttt gagacaccgc gcttgcggaa gcatcagcaa
    10621 ataaggccag cacagccagc gcagttgccg ctttggttcc tgattctgtt cttgatgaat
    10681 taaacaaagc ggcacagtaa caaaggactt cattgataat ttttcttcag gaggaagaca
    10741 tgtcattctt ttctacgtta aaaacagctt tgtctttgaa ggagaaactt gctgctactg
    10801 gtgttcttgt tctgatttgc gcacttgttg gtgctgggtt tgcatgggaa cgtcatcagc
    10861 taaagcaagc catagagaaa attggcagtc ttgatcaggc tgttaaggaa cgtgataagt
    10921 caataatgga tcttaaccag accattgaga cgatgaacaa agcagagcaa cattttcaca
    10981 gccaggaagt gaaaaatgaa tcagaacaag ccaagtatgc tgacaggcaa atggaacgaa
    11041 aagctgaagt tcagaaacaa ctggttgcgg cgggtaatgt tcgccagcgt attcctgctg
    11101 acactcagcg gttgctccgg gagtcgatca gcgaatttaa cgccgacgcc gacaaaggtt
    11161 aaccaccctg cccccaaaag tgcatttatg tgtaggatgc cagagtttag cagtgaatat
    11221 tttgatgatc tgccagcgta tatcctcgat acagaaacga tgctgatggg gattaacagg
    11281 aagaatcgca acgttaatga ttacaaccga gctattagcg gtaactaaaa gggattttta
    11341 tgtctgataa agtaacagta aagcaaacta tcaacaaagc gacttcaatc tacaaaattg
    11401 agcaaatcac tgttggcaag ccaggatctg aacaataccg tcgtgctttc gagcttgccg
    11461 atcagcttgg tttaaaacac ccggattgca ttgagcatgt atttccgacc tatgctgatg
    11521 agcaatgtac tcatgttctt accgaagagg attttttcag cactgaagaa cgagaaggcg
    11581 ttgatcgctg cattggtgtg atttgttctt cggtaagtga tgagttattc cctaatgtgc
    11641 ctgaatatgg tggtattgga taccaattcc tgtacgaggg cgatgagctt aaatgctatg
    11701 aacatggtct tctcatcgaa agcgtagaat aatacgactc ccttccaacc ggctacgttg
    11761 gccggttttt cacttatcca cattatccac tggatagatc caataatcag gtccatacag
    11821 atcccaatta gatccatata gatccctgat cgttgcaggc cgcgccacgt ctggcttaga
    11881 agtgtatcgc gatgtgtgct ggagggaaaa cgatgtgtgc tggagggata aaaatgtgtg
    11941 ctgacgggtt gctaatgtgt gctggcggga tataggatgt gtgttgacgg gaaagctagc
    12001 ttgggtagtt atcaccactt ataaaaacta tccacacaat tcggaaaaag taatatgaat
    12061 caatcattta tctccgatat tctttacgca gacattgaaa gtaaggcaaa agaactaaca
    12121 gttaattcaa acaacactgt gcagcctgta gcgttgatgc gcttgggggt attcgtgccg
    12181 aagccatcaa agagcaaagg agaaagtaaa gagattgatg ccaccaaagc gttttcccag
    12241 ctggagatag ctaaagccga gggttacgat gatattaaaa tcaccggtcc tcgactcgat
    12301 atggatactg atttcaaaac gtggatcggt gtcatctacg cgttcagcaa atacggcttg
    12361 tcctcaaaca ccatccagtt atcgtttcag gaattcgcta aagcctgtgg tttcccctca
    12421 aaacgtctgg atgcgaaact gcgtttaacc attcatgaat cacttggacg cttgcgtaac
    12481 aagggtatcg cttttaagcg cggaaaagat gctaaaggcg gctatcagac tggtctgctg
    12541 aaggtcgggc gttttgatgc tgaccttgat ctgatagagc tggaggctga ttcgaagttg
    12601 tgggagctgt tccagcttga ttatcgcgtt ctgttgcaac accacgcctt gcgtgccctt
    12661 ccgaagaaag aagctgcaca agccatttac actttcatcg aaagccttcc gcagaacccg
    12721 ttgccgctat cgttcgcgcg aatccgtgag cgcctggctt tgcagtcagc tgttggcgag
    12781 caaaaccgta tcattaagaa agcgatagaa cagcttaaaa caatcggcta tctcgactgt
    12841 tctattgaga agaaaggccg ggaaagtttt gtaatcgtcc attctcgcaa tccaaagctg
    12901 aaactccccg aataagtgtg tgctggaggg aaaccgcatt aaaaagatgt gtgctgccgg
    12961 gaaggcttgt ccaatttcct gtttttgatg tgcgctggag ggggacgccc ctcagtttgc
    13021 ccagactttc cctccagcac acatctgtcc atccgctttt ccctccagca cacatcgaag
    13081 ctgccgggca agccgttctc accagttgat agagagtgaa gctagcttgg ctgcccattg
    13141 aagcaggaaa tcaccaaaat gattcaggct acaacctgaa cgtagaagaa atccgcgtcc
    13201 tttatgcgtg gaggatgcca aagcatgttg tgacacactt ggcaaaggag taagcatgca
    13261 gagaatgcta tgtacaagca tctacgcata cattattatt ttatgcagca tttttaatta
    13321 aattcaaaaa tacagcataa aggatgactt tcgatgagtg attccagcca gcttcacaag
    13381 gttgctcaaa gagcaaacag aatgctcaat gttctgactg aacaagtaca gttgcaaaag
    13441 gatgagctac acgcgaacga gttttaccag gtctatgcga aagcggcact ggcaaaattg
    13501 cctctactga ctcgagcgaa cgttgactat gccgtaagtg aaatggaaga aaagggttat
    13561 gttttcgata aacgccctgc tggctcttca atgaaatatg cgatgtcaat tcagaacatc
    13621 attgacatat atgaacatcg cggagtgcca aaataccggg atcgctacag cgaagcgtat
    13681 gtgattttca tctccaatct taaaggcggt gtgtcaaaaa ctgtatcgac ggtttctctg
    13741 gcgcatgcaa tgcgtgctca ccctcatctt cttatggagg atttaaggat tctggttatt
    13801 gaccttgatc cgcaatcttc agcaacgatg tttttaagcc ataaacactc tattggtatc
    13861 gtaaacgcaa catctgcaca ggctatgttg cagaatgtaa gccgtgaaga gctgttagag
    13921 gagtttattg ttccttctgt tgtacctggg gttgacgtta tgcctgcgtc gattgacgat
    13981 gcctttattg catccgattg gagagagctg tgcaatgagc atctaccggg tcagaacatc
    14041 catgctgtcc tgaaagaaaa tgtgattgat aagctgaaga gcgattatga ctttatcctc
    14101 gttgatagtg gtcctcacct tgacgccttc ctgaaaaatg ctttggcctc ggccaatata
    14161 ctgtttacac ctctgccgcc agcaactgtc gatttccact catcgcttaa atacgttgcc
    14221 cgccttcctg agttggtgaa actcatttcg gatgaaggct gcgagtgcca gcttgcgact
    14281 aacattggtt ttatgtccaa gttgagtaac aaggcagacc ataagtattg ccatagcctg
    14341 gctaaagaag tgttcggtgg ggatatgctt gatgtcttcc tccctcgcct tgacggtttt
    14401 gaacgctgcg gcgagtcttt tgacactgtt atttcagcta acccggcaac gtatgttggt
    14461 agtgctgatg cattgaagaa cgcgcgaatt gccgcggaag attttgctaa agcagttttt
    14521 gaccgtattg aatttatcag atctaactga ggagtaagaa acccccatgt caaagaaaaa
    14581 cagaccaaca attgggcgaa cccttaatcc ttcaatatta agcggatttg atagttcttc
    14641 agcctctggc gatcgagtcg agcaggtatt caagttatca actggtcgcc aggccacatt
    14701 tattgaagag gtaatacctc cgaaccaggt agaaagcgat acctttgttg atcagcataa
    14761 caacgggcgt gaccaggcat ctcttacgcc aaaatcatta aaaagtatcc gaagcactat
    14821 taagcatcag caattttacc ctgcaatagg tgttagacgg gctacaggga aaattgaaat
    14881 tttggatggt tcccggcgtc gagcttctgc catcttagag aacgtagggt tgcgggtttt
    14941 agtcacggac caggagatca gcgttcagga agcgcaaaat ttagcgaaag acgttcagac
    15001 agcattgcag cacagcattc gagaaatagg tctgcgtttg atgcgaatga aaaatgatgg
    15061 gatgagtcag aaggatattg cagccaaaga agggctgtct caggcgaagg tcacgcgtgc
    15121 tctccaggca gcgagtgctc cggaagaatt agtcgccctt ttccctgtgc agtcggaatt
    15181 aaccttttcg gactacaaaa cgctttgtgc tgttggcgac gaaatgggga acaagaattt
    15241 agagtttgat cagcttattc aaaacatatc cccggaaata aacgacatct tatccattga
    15301 agaaatggcc gaagatgaag ttaaaaataa aatcctgcgc ttgataacaa aggaagcctc
    15361 actactcacg gataaaggtt ctaaagataa gtccgtagtt actgaattat ggaaatttga
    15421 ggacaaggat cgctttgcaa ggaagcgcgt gaaaggccgt gcattttctt atgagtttaa
    15481 tcgactctca aaagagttac aggaagaact cgacaggatg attgggcata tccttagaaa
    15541 gagcctcgat aaaaagccga agccttaaac tttcgccatt caaatttcac tattaactga
    15601 ctgtttttaa agtaaattac tctaaaattt caaggtgaaa tcgccacgat ttcaccttgg
    15661 attttacctt cctcccctcc tcccgaaaaa aataaaaaaa ttgcttgtca cgagaaagtc
    15721 aacaagtgac tttcaataaa atctcttccg aaaagggatt cacacaagtg ccttgtgttt
    15781 aaggaagagt aaattgagta acttacgcga ataccagaat cgtattgcag atatcgcaaa
    15841 acgctctaaa gctgtgcttg gctgggcaag cactgcgcag ttcggtactg ataaccaatt
    15901 cattaaagat gatgccgcgc gtgccgcatc tatccttgaa gctgcacgta aagacccggt
    15961 ttttgcgggt atctctgata atgccaccgc tcaaatcgct acagcgtggg caagtgcact
    16021 ggctgactac gccgcagcac ataaatctat gccgcgtccg gaaattctgg cctcctgcca
    16081 ccagacgctg gaaaactgcc tgatagagtc cacccgcaat agcatggatg ccactaataa
    16141 agcgatgctg gaatctgtcg cagcagagat gatgagcgtt tctgacggtg ttatgcgtct
    16201 gcctttattc ctcgcgatga tcctgcctgt tcagttgggg gcagctaccg ctgatgcgtg
    16261 taccttcatt ccggttacgc gtgaccagtc cgacatctat gaagtcttta acgtggcagg
    16321 ttcatctttt ggttcttatg ctgctggtga tgttctggac atgcaatccg tcggtgtgta
    16381 cagccagtta cgtcgccgct atgtgctggt ggcaagctcc gatggcacca gcaaaaccgc
    16441 aaccttcaag atggaagact tcgaaggcca gaatgtacca atccgaaaag gtcgcactaa
    16501 catctacgtt aaccgtatta agtctgttgt tgataacggt tccggcagcc tacttcactc
    16561 gtttactaat gctgctggtg agcaaatcac tgttacctgc tctctgaact acaacattgg
    16621 tcagattgcc ctgtcgttct ccaaagcgcc ggataaaggc actgagatcg caattgagac
    16681 ggaaatcaat attgaagccg ctcctgagct gatcccgctg atcaaccacg aaatgaagaa
    16741 atacaccctg ttcccaagtc agttcgttat cgcggctgag cacacggtac aggcggcgta
    16801 tgaagcacat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    16861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    16921 tgtaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    16981 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    17041 gattacgcca agctagcttg catgcctgca ggtcgaaggc cgtggcggcg gcacttttgc
    17101 ccatgaattg ctcgctgtgg agtacgcagg ctggatttct cccgcgtttc ggctgaaggt
    17161 aaaccagaca tttatcgact atcgaaccgg aagattacaa cctgctattc cgcagagtct
    17221 ccctgaagct ctccgtttgg ctgccgacct ggcagagcaa aagcaacggc tggagcaaaa
    17281 aatgcttatg gatgcaccta aagtcgaatt cgctgaacgc gttgctaccg ccagcggggt
    17341 tctaatcggc aactatgcca aagtgctcgg cctgggccaa aactatctct tcacctggtt
    17401 gcgtgataac ggaattctga tcgcaaccgg tgaacgcagg aacgtcccca aacaagaata
    17461 catatctcgt gggtatttca cccttaaaga aaccgtgatc gatacaagca atggaagcag
    17521 gatttctttc acgactcgta taaccggcaa aggtcagcag tggctgatga agcgattgct
    17581 tgatgctggt gtgctgctgt cgcggcaacg cgctaacaga cgtagtaaga accaccagca
    17641 ttgtaatgct ggctaaagtc actttcctga gctgtataac gatgagcgat tttacttttt
    17701 ctggctatga attggcctgc tttgtaacac actccggtct atcccgtagc gccgggcata
    17761 tcctgtcgca atgtgcaaat ctcgcggcaa caaccagtga atacttcatt cacaagcctc
    17821 accgcctgat cgcggcagaa actggttata gccaatcaac cgtcgttcgt gcattccgtg
    17881 aagctgtaaa caaaggaatt ctgtctgtag agattgttat cggcgatcac cgtgaacgtc
    17941 gcgctaacct gtaccggttt acaccatcct ttttggcctt cgcacaacaa gccaaaaatg
    18001 cgctgataga aagcaaatta aagatctctt cagcggcaac caaggttaaa gctgttctcg
    18061 ctaagacatt ggctttattt aattttttat ccacaccccc atgtcaaaat gataccccct
    18121 ccccctgtca ggatgacgtg gcaataaaga ataagaagtc acaagttaaa aaaacaaaaa
    18181 gatcagtttc cggcggtgcc ggaacaacca gcctcaaaaa attgacttca tggatcgcta
    18241 aggcaaaagc aaaggctgac aatctgcggt tatccaaaaa acgcactcaa aaacatgagt
    18301 tcaagcagaa agtagaggcg gctgcgcgga aatatgctta cctgaagaac aagcgttcgc
    18361 ctgatattgg cgggatatca aacttcgata acctaccgca ttgcatgacg gtaaacgaag
    18421 ctcttaatgc ggttttagcc aaaaataaag ataacgaaca atggggtata ccggcaggat
    18481 tcagagggta atgaattgct ctaattataa ccatgcatac tttcaacacc tctagtttgc
    18541 catgaggcaa actcataggt gtcctggtaa gaggacactg ttgccaaaac tggacgcccc
    18601 attattgcaa ttactaagaa accattatta tcatgacatt aacctataaa aataggcgta
    18661 tcacgaggcc ctttcgtctt caagaatttt ataaaccgtg gagcgggcaa tactgagctg
    18721 atgagcaatt tccgttgcac cagtgccctt ctgatgaagc gtcagcacga cgttcctgtc
    18781 cacggtacgc ctgcggccaa atttgattcc tttcagct
//
