LOCUS       sequence-487372-       12446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses HSPD1 tagged with HaloTag2 at the relative 
            location of intron 7 in the HSPD1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Sansbury SE, Serebrenik YV, Lapidot T, Burslem GM, Shalem O
  TITLE     Pooled tagging and hydrophobic targeting of endogenous proteins for 
            unbiased mapping of unfolded protein responses.
  JOURNAL   bioRxiv. 2023 Jul 14:2023.07.13.548611. doi: 
            10.1101/2023.07.13.548611. Preprint.
  PUBMED    37503003
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jul 14:2023.07.13.548611. doi: 10.1101/2023.07.13.548611. 
            Preprint."
COMMENT     Sequence Label: HSPD1-i7
            -HT2
FEATURES             Location/Qualifiers
     source          1..12446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2652..2966
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2660..2678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2696..2714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2731..2749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2767..2785
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2803..2821
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2838..2856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2874..2892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2939..2963
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5629..5685
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5686..6486
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5746..5765)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6356..6375
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        6542..6753
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(6778..7395,7396..7401,7402..7437,7438..7440,7441..7476,
                     7477..7479,7480..7515,7516..7524)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7564..7584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7982..7993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8198..8431
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8457..8474)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8463..8687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8733..9161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8820..8839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9030..9051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9170..9190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9175..9504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9355..9490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9417..9436
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9552..9599
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9618..9992
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10122..10243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10159..10178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10213..10232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10292..10308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10292..10308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10305..10327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10316..10332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10340..10346,10347..10364,10365..10370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10385..10406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10523..10540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10694..11282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10774..10793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11453..12244,12245..12313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12076..12095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12314..12418)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg ggtttattac agggacagca gagatccact ttggccgcga
     2641 atcgatatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     2701 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2761 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     2821 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2881 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2941 ctcgtttagt gaaccgtcag atcgcctgga gaattggcac cgatgcttcg gttacccaca
     3001 gtctttcgcc agatgagacc ggtgtccagg gtactggctc ctcatctcac tcgggcttat
     3061 gccaaagatg taaaatttgg tgcagatgcc cgagccttaa tgcttcaagg tgtagacctt
     3121 ttagccgatg ctgtggccgt tacaatgggg ccaaagggaa gaacagtgat tattgagcag
     3181 agttggggaa gtcccaaagt aacaaaagat ggtgtgactg ttgcaaagtc aattgactta
     3241 aaagataaat acaaaaacat tggagctaaa cttgttcaag atgttgccaa taacacaaat
     3301 gaagaagctg gggatggcac taccactgct actgtactgg cacgctctat agccaaggaa
     3361 ggcttcgaga agattagcaa aggtgctaat ccagtggaaa tcaggagagg tgtgatgtta
     3421 gctgttgatg ctgtaattgc tgaacttaaa aagcagtcta aacctgtgac cacccctgaa
     3481 gaaattgcac aggttgctac gatttctgca aacggagaca aagaaattgg caatatcatc
     3541 tctgatgcaa tgaaaaaagt tggaagaaag ggtgtcatca cagtaaagga tggaaaaaca
     3601 ctgaatgatg aattagaaat tattgaaggc atgaagtttg atcgaggcta tatttctcca
     3661 tactttatta atacatcaaa aggtcagaaa tgtgaattcc aggatgccta tgttctgttg
     3721 agtgaaaaga aaatttctag tatccagtcc attgtacctg ctcttgaaat tgccaatgct
     3781 caccgtaagc ctttggtcat aatcgctgaa gatgttgatg gagaagctct aagtacactc
     3841 gtcttgaata gtggtggctc tggaagttca tccgaaatcg gtacaggctt ccccttcgac
     3901 ccccattatg tggaagtcct gggcgagcgt atgcactacg tcgatgttgg accgcgggat
     3961 ggcacgcctg tgctgttcct gcacggtaac ccgacctcgt cctacctgtg gcgcaacatc
     4021 atcccgcatg tagcaccgag tcatcggtgc attgctccag acctgatcgg gatgggaaaa
     4081 tcggacaaac cagacctcga ttatttcttc gacgaccacg tccgctacct cgatgccttc
     4141 atcgaagcct tgggtttgga agaggtcgtc ctggtcatcc acgactgggg ctcagctctc
     4201 ggattccact gggccaagcg caatccggaa cgggtcaaag gtattgcatg tatggaattc
     4261 atccggccta tcccgacgtg ggacgaatgg ccagaattcg cccgtgagac cttccaggcc
     4321 ttccggaccg ccgacgtcgg ccgagagttg atcatcgatc agaacgcttt catcgagggt
     4381 gcgctcccga tgggggtcgt ccgtccgctt acggaggtcg agatggacca ctatcgcgag
     4441 cccttcctca agcctgttga ccgagagcca ctgtggcgat tccccaacga gctgcccatc
     4501 gccggtgagc ccgcgaacat cgtcgcgctc gtcgaggcat acatgaactg gctgcaccag
     4561 tcacctgtcc cgaagttgtt gttctggggc acacccggcg tactgatccc cccggccgaa
     4621 gccgcgagac ttgccgaaag cctccccaac tgcaagacag tggacatcgg cccgggattg
     4681 ttcttgctcc aggaagacaa cccggacctt atcggcagtg agatcgcgcg ctggctcccc
     4741 gggctggccg gcggaagtag tggaagttcg ctaaaggttg gtcttcaggt tgtggcagtc
     4801 aaggctccag ggtttggtga caatagaaag aaccagctta aagatatggc tattgctact
     4861 ggtggtgcag tgtttggaga agagggattg accctgaatc ttgaagacgt tcagcctcat
     4921 gacttaggaa aagttggaga ggtcattgtg accaaagacg atgccatgct cttaaaagga
     4981 aaaggtgaca aggctcaaat tgaaaaacgt attcaagaaa tcattgagca gttagatgtc
     5041 acaactagtg aatatgaaaa ggaaaaactg aatgaacggc ttgcaaaact ttcagatgga
     5101 gtggctgtgc tgaaggttgg tgggacaagt gatgttgaag tgaatgaaaa gaaagacaga
     5161 gttacagatg cccttaatgc tacaagagct gctgttgaag aaggcattgt tttgggaggg
     5221 ggttgtgccc tccttcgatg cattccagcc ttggactcat tgactccagc taatgaagat
     5281 caaaaaattg gtatagaaat tattaaaaga acactcaaaa ttccagcaat gaccattgct
     5341 aagaatgcag gtgttgaagg atctttgata gttgagaaaa ttatgcaaag ttcctcagaa
     5401 gttggttatg atgctatggc tggagatttt gtgaatatgg tggaaaaagg aatcattgac
     5461 ccaacaaagg ttgtgagaac tgctttattg gatgctgctg gtgtggcctc tctgttaact
     5521 acagcagaag ttgtagtcac agaaattcct aaagaagaga aggaccctgg aatgggtgca
     5581 atgggtggaa tgggaggtgg tatgggaggt ggcatgttcg gatccggcgc aacaaacttc
     5641 tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgggatc ggccattgaa
     5701 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5761 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5821 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5881 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5941 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6001 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6061 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6121 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6181 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6241 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6301 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6361 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6421 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6481 ttctgagaat tcgctagcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     6541 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6601 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6661 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6721 ctttttcgca acgggtttgc cgccagaaca caggaccggt tctagagcgc tgccaccatg
     6781 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     6841 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     6901 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     6961 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     7021 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     7081 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     7141 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     7201 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     7261 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     7321 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     7381 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     7441 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
     7501 gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa
     7561 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7621 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7681 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7741 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7801 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7861 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7921 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7981 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8041 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     8101 tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct
     8161 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8221 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8281 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8341 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8401 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     8461 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8521 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8581 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8641 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8701 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     8761 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8821 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8881 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8941 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9001 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9061 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9121 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     9181 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9241 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9301 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9361 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9421 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9481 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     9541 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     9601 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     9661 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     9721 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     9781 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     9841 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     9901 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     9961 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
    10021 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10081 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10261 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12421 catttccccg aaaagtgcca cctgac
//
