LOCUS       sequence-487376-       11981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses CALR tagged with HaloTag2 at the relative 
            location of intron 2 in the CALR gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Sansbury SE, Serebrenik YV, Lapidot T, Burslem GM, Shalem O
  TITLE     Pooled tagging and hydrophobic targeting of endogenous proteins for 
            unbiased mapping of unfolded protein responses.
  JOURNAL   bioRxiv. 2023 Jul 14:2023.07.13.548611. doi: 
            10.1101/2023.07.13.548611. Preprint.
  PUBMED    37503003
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jul 14:2023.07.13.548611. doi: 10.1101/2023.07.13.548611. 
            Preprint."
COMMENT     Sequence Label: CALR-i2-HT2
FEATURES             Location/Qualifiers
     source          1..11981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2652..2966
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2660..2678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2696..2714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2731..2749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2767..2785
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2803..2821
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2838..2856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2874..2892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2939..2963
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5164..5220
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5221..6021
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5281..5300)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5891..5910
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        6077..6288
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(6313..6930,6931..6936,6937..6972,6973..6975,6976..7011,
                     7012..7014,7015..7050,7051..7059)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7099..7119)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7517..7528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7733..7966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7992..8009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7998..8222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8268..8696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8355..8374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8565..8586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8705..8725)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8710..9039
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8890..9025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8952..8971
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9087..9134
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9153..9527
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9657..9778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9694..9713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9748..9767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9827..9843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9827..9843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9840..9862)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9851..9867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9875..9881,9882..9899,9900..9905))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9920..9941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10058..10075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10229..10817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10309..10328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10988..11779,11780..11848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11611..11630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11849..11953)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg ggtttattac agggacagca gagatccact ttggccgcga
     2641 atcgatatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     2701 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2761 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     2821 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2881 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2941 ctcgtttagt gaaccgtcag atcgcctgga gaattggcac cgatgctgct atccgtgccg
     3001 ctgctgctcg gcctcctcgg cctggccgtc gccgagcctg ccgtctactt caaggagcag
     3061 tttctggacg gagacgggtg gacttcccgc tggatcgaat ccaaacacaa gtcagatttt
     3121 ggcaaattcg ttctcagttc cggcaagttc tacggtgacg aggagaaaga taaagctggt
     3181 ggctctggaa gttcatccga aatcggtaca ggcttcccct tcgaccccca ttatgtggaa
     3241 gtcctgggcg agcgtatgca ctacgtcgat gttggaccgc gggatggcac gcctgtgctg
     3301 ttcctgcacg gtaacccgac ctcgtcctac ctgtggcgca acatcatccc gcatgtagca
     3361 ccgagtcatc ggtgcattgc tccagacctg atcgggatgg gaaaatcgga caaaccagac
     3421 ctcgattatt tcttcgacga ccacgtccgc tacctcgatg ccttcatcga agccttgggt
     3481 ttggaagagg tcgtcctggt catccacgac tggggctcag ctctcggatt ccactgggcc
     3541 aagcgcaatc cggaacgggt caaaggtatt gcatgtatgg aattcatccg gcctatcccg
     3601 acgtgggacg aatggccaga attcgcccgt gagaccttcc aggccttccg gaccgccgac
     3661 gtcggccgag agttgatcat cgatcagaac gctttcatcg agggtgcgct cccgatgggg
     3721 gtcgtccgtc cgcttacgga ggtcgagatg gaccactatc gcgagccctt cctcaagcct
     3781 gttgaccgag agccactgtg gcgattcccc aacgagctgc ccatcgccgg tgagcccgcg
     3841 aacatcgtcg cgctcgtcga ggcatacatg aactggctgc accagtcacc tgtcccgaag
     3901 ttgttgttct ggggcacacc cggcgtactg atccccccgg ccgaagccgc gagacttgcc
     3961 gaaagcctcc ccaactgcaa gacagtggac atcggcccgg gattgttctt gctccaggaa
     4021 gacaacccgg accttatcgg cagtgagatc gcgcgctggc tccccgggct ggccggcgga
     4081 agtagtggaa gttctggttt gcagacaagc caggatgcac gcttttatgc tctgtcggcc
     4141 agtttcgagc ctttcagcaa caaaggccag acgctggtgg tgcagttcac ggtgaaacat
     4201 gagcagaaca tcgactgtgg gggcggctat gtgaagctgt ttcctaatag tttggaccag
     4261 acagacatgc acggagactc agaatacaac atcatgtttg gtcccgacat ctgtggccct
     4321 ggcaccaaga aggttcatgt catcttcaac tacaagggca agaacgtgct gatcaacaag
     4381 gacatccgtt gcaaggatga tgagtttaca cacctgtaca cactgattgt gcggccagac
     4441 aacacctatg aggtgaagat tgacaacagc caggtggagt ccggctcctt ggaagacgat
     4501 tgggacttcc tgccacccaa gaagataaag gatcctgatg cttcaaaacc ggaagactgg
     4561 gatgagcggg ccaagatcga tgatcccaca gactccaagc ctgaggactg ggacaagccc
     4621 gagcatatcc ctgaccctga tgctaagaag cccgaggact gggatgaaga gatggacgga
     4681 gagtgggaac ccccagtgat tcagaaccct gagtacaagg gtgagtggaa gccccggcag
     4741 atcgacaacc cagattacaa gggcacttgg atccacccag aaattgacaa ccccgagtat
     4801 tctcccgatc ccagtatcta tgcctatgat aactttggcg tgctgggcct ggacctctgg
     4861 caggtcaagt ctggcaccat ctttgacaac ttcctcatca ccaacgatga ggcatacgct
     4921 gaggagtttg gcaacgagac gtggggcgta acaaaggcag cagagaaaca aatgaaggac
     4981 aaacaggacg aggagcagag gcttaaggag gaggaagaag acaagaaacg caaagaggag
     5041 gaggaggcag aggacaagga ggatgatgag gacaaagatg aggatgagga ggatgaggag
     5101 gacaaggagg aagatgagga ggaagatgtc cccggccagg ccaaggacga gctgggatcc
     5161 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     5221 ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5401 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5701 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5881 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6001 cttcttgacg agttcttctg agaattcgct agctaggtct tgaaaggagt gggaattggc
     6061 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     6121 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     6181 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     6241 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggttctag
     6301 agcgctgcca ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta
     6361 ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     6421 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     6481 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     6541 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     6601 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     6661 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     6721 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     6781 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     6841 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     6901 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac
     6961 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     7021 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag
     7081 atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7141 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7201 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7261 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7321 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7381 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7441 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7501 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7561 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7621 ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg
     7681 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7741 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7801 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7861 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7921 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     7981 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8041 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8101 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8161 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8221 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     8281 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8341 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8401 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8461 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8521 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8581 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8641 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     8701 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8761 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8821 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8881 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8941 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     9001 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     9061 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     9121 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     9181 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     9241 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     9301 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     9361 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     9421 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     9481 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     9541 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     9601 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     9661 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9721 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9781 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9841 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9901 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9961 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10021 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10081 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10141 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11941 taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
