LOCUS       sequence-487606-       18033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA expressing plasmid with broken SEPARATOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18033)
  TITLE     High-throughput production of genetically sterile males for 
            population suppression of Anopheles gambiae in Africa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 1197R_gZBF-ER
FEATURES             Location/Qualifiers
     source          1..18033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_RNA        1818..1893
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    1923..1956
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5583..5585,5586..6260)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5763..5783)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5981..6000
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6175..6198
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    6381..6502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        6857..6932
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        7324..7399
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(10129..10131,10132..10134,10135..10845)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10174..10195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10435..10454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10782..10803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10975..11056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    11109..11142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     11146..11162
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     11147..11163
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   11446..11480
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11971..11987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11971..11987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11984..12006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11995..12011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12019..12025,12026..12043,12044..12049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12064..12085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12202..12219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16355..16943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16435..16454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(17161..17180)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             17172..17981
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(17239..17258)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcggtcgt gacgtagatt cgtcgccaca tatacactga
     1021 agcggtttgg ctcttggtta ttctagaaaa ctctgttttt gaattaatca taggaaaact
     1081 gtttattctt ttttgatatt tcaacgtatt acaatgaata gtaacttttg attaaaaaag
     1141 agatgaagca atacaagaaa tgttgaaaag tttatgaaac atcacattag cgtgagttac
     1201 ggcaggatca aaaccttata acagtcacac tcaggcaaaa aatccttctt gaatatcctt
     1261 tatgcatcgc tagagcaagg attgaaagcg caaagtatat atacaacctt ttttcccctc
     1321 gtccttgatc gagcgcgtcg cctggtagtt ccagagctat ggaaacatag caagttggaa
     1381 taaggcttag tccgtactca acttgaaaaa gtggcaccga gtcggtgcat ttttttcggt
     1441 gactatcccc cacggtaagt gtctatcacc cctagccaca tatacactga agcggtttgg
     1501 ctcttggtta ttctagaaaa ctctgttttt gaattaatca taggaaaact gtttattctt
     1561 ttttgatatt tcaacgtatt acaatgaata gtaacttttg attaaaaaag agatgaagca
     1621 atacaagaaa tgttgaaaag tttatgaaac atcacattag cgtgagttac ggcaggatca
     1681 aaaccttata acagtcacac tcaggcaaaa aatccttctt gaatatcctt tatgcatcgc
     1741 tagagcaagg attgaaagcg caaagtatat atacaacctt ttttcccctc gtccttgctg
     1801 ggccaaaggg cactacagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     1861 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg gccgtttccc ggatataaac
     1921 atataacttc gtataatgta tgctatacga agttatggat cctaatttta accgcctttc
     1981 ggaacatcaa cattccaacc ggtgacagga taggatcgaa ttcggctcgg agcttgcttg
     2041 atcggattgt ccatactgaa cattttcttg ctctatcgtg ggtgggtgaa attgatcgaa
     2101 ttgatttctg gaatattttc gatgatgaaa atttggtccc atttttgttc tttgcaaaac
     2161 cactatttga gaaaaagatg tggcatcaac acagatacaa aataatgaat agcaattgat
     2221 gggtaacaac aacaaaacgg cgaaaagaag ctttgctgtc ttgctggaga acttccggtc
     2281 aagtgtgcgt ttttggaagc cgcgttttgt gcctagtgat gggcgaatcg agggatggtc
     2341 atcaggcact ggaaccaatt tttttttttg tatttttaac aaaaaaggtt ttatgtttta
     2401 aaaatcgatt aaaacagtaa aacttaaaaa atcaattatt ttctttagta aatttgatag
     2461 gccatcttac tttttttaaa tttgttaaac attttctgtg tagctgaata aaatgtatta
     2521 caatatacga tccttcaaat cctattccct tatgtgttct attcacacac cagcttaaga
     2581 ctttggttaa aaaaaaaata taatgaatca aatagaattg taggttgatg attttcaaaa
     2641 agaacgatta aaaaacaatt ttatccacca acagcctaga tcagcaggtt cgtatacgga
     2701 atgcaatttc acaactggaa tcaaattccg caatccgtaa cggttgacat ttacttgctg
     2761 tcgaaatgaa ttgctccatc cagcacgcac gaaggtcatt tgaatttgct gctctaaaac
     2821 gtcatgcccg gaactatcgt gcacgcggaa gcgaaagcaa tgagcgatcc ttgcgctata
     2881 gtgcgtgcca taaatacgtt ggcgccagcc attttcttcc ttcttttgtt cgtgtctctc
     2941 gccggataaa gtcgttcgct agcccctttg tttgaattcg aaaatgcccc tgatgtacgt
     3001 catactaaag agcgccgatg gcgatgtaca aaaagcacac cagcggatcg acgaaggtaa
     3061 gctggcgatg atggtgtcgt tcgacatcac tttcatcacc gtgtcagaca tctactgtgc
     3121 ctagcaccgg gtccagtggt cacagggtgt agcaaaaacg tgttcttttt tgcgagagac
     3181 tctacctcat gatgcagctg ttaaggaaag gtttcagttg aaggcaattt ttcctaggat
     3241 aagatgatct taagttacct gcgtattagt gtttaacatt gtcgtctcaa ctcccaagaa
     3301 tgttttaatc gtctagggtt agtttatgta tactgttctc attgaaatgt cgttcaatcc
     3361 aacatgttaa gttagctagc tcagacacga gaagttagga gtatctgcat cttgaaggta
     3421 gcggcatatg gtgttatgcc acgttcactg acttcaaaat tcgatatata aaaaaaacaa
     3481 aatcaaaaac aaaattgtga attccgtcag ccagcagcag tgaccttcaa agccttacct
     3541 ttccattcat ttatgtttaa cacaggtcaa gcggtggtca acgaatactc acgattgcat
     3601 aatctgaaca tgtttgatgg cgtggagttg cgcaatacca cccgtcagag tggatgataa
     3661 actttccgca ccactgtaac tgtccgtatc tttgtatgtg ggtgtgtgta tgtgtgtttg
     3721 gtgaaacgaa ttcaatagtt ctgtgctatt ttaaatcaag ccgcgtgcgc aactgatgcc
     3781 gataagttca aactagtgtt taaggagtgg agcgagagag ccgcaccacg gtacagaagg
     3841 gcagcagaat gggtcggcag cctagctgca ctggtgcggt gcgtccggca tctcggggag
     3901 agggcgggga aattctagtg ttaaatcgga gcagcaaaaa caaaacagtg gtcgtcccgt
     3961 tcaagaaacg gcctatacac acacagaaaa cactgcagca tgtttgtaca tagtagatcc
     4021 tagagcaggt ggtcgctgct cctcgaacgc tctggacgca cgactttgcg cgtacttgcg
     4081 tagcgttccg ccgatcgtgg gtattcgtac tgccacaagc ccgctttctc ccatgcaatc
     4141 tctgcaacca aaccaacaaa caacaacaaa aaaccaatcg acacaatgaa tcacacccct
     4201 tttgtatcat ctgtatattt cttgttcttt gcgttctttt ctatgtggcc cacgccccgg
     4261 cgggtacgta attgcgtcga aaaccccgaa aaccccggca catacagtgt acatacggtt
     4321 tgaggacaac tttgacctgc agcccttctg gggttgccac gtgtagctat acttgtgaga
     4381 tcgggcgccg acggtgtaaa gcgcgaatgg ccgccacaca gtgtgtccac tccaacacta
     4441 cccctctgga actaccccgt ccagggatgc accggctcgg ctcatgcccc tgcaaaacag
     4501 tccgggctcc actgtagtag ctccggcgtt gctctgagag aaggatgccc ttcgaagtgt
     4561 cgaaagcgtg cattgggcgt tcaagtgtgt gtgtgtgtgt taggtttagc gagaaacagc
     4621 agcagttgcg tgtgctgaaa agcgaaggag taatagagtg cataatgaaa atgaaaatga
     4681 aaatgaagca aaagtagaag gcggaggaga gcaacctgtg ttccactagt agcgaatagt
     4741 ttagtctagt ttcgtcacca atcaaccttc caaccatcgt tcaaccaata cctgagtcaa
     4801 catcgtcatc gttatcgtgc cacaacttta ttaaaaatga accttgtccg cgccaccgta
     4861 gggtgatctg agacgacctt tcttacgggc gcgacccaca tgccatcgtc accttctcca
     4921 atcaaaacca acagcctgta ccgatggtgt gcaattgtgc gtgcgtgtgt gttattagca
     4981 aaaaaagaga aagagacggc tagagagaga tagatcgaga tcgagagtac aaaagagcag
     5041 tagaaatgtt cgttgtttgt tttccgtaac acagttgttc agccaaaatg ggaattttca
     5101 acaatcccgg gggcggggaa atgcgggaat actgcgtaca cacatacatc aatcaaaaag
     5161 aaaaatcctt gcgctacatc actaccgttt gcgcggtgct gatctagagc agaccacttt
     5221 ccactccact ctacaatcaa tcaatctgtg cagaaggtat ggtaagacgg cctttgagcg
     5281 agtcacggtc gccaccataa cgccgtccga cgagggctga atgcgaactt tgctaatcga
     5341 ttttccgctt tctttttatc ccacccccct ttctctctct ctctctcttt tgcaccgccc
     5401 cttgtaaccc ccaaaaaggt aaacgacaca ttaagaccta cgaagcgctg gtgaagtcat
     5461 cgctcgatcc gaacagcgac cggctgacgg aggacgacga cgaggacgag aacatctcgg
     5521 tgacccgcac caactccacc attcggtcga ggtccagctc gctgtcgccg gcaccggcac
     5581 cggtgcgctc ctccaagaac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     5641 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5701 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     5761 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     5821 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5881 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
     5941 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga
     6001 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     6061 agatccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
     6121 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg
     6181 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
     6241 gccaccacct gttcctgtag cggccgcgac tctagatcat aatcagccat accacatttg
     6301 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     6361 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     6421 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6481 ccaaactcat caatgtatct taacctctca atccccacat atacactgaa gcggtttggc
     6541 tcttggttat tctagaaaac tctgtttttg aattaatcat aggaaaactg tttattcttt
     6601 tttgatattt caacgtatta caatgaatag taacttttga ttaaaaaaga gatgaagcaa
     6661 tacaagaaat gttgaaaagt ttatgaaaca tcacattagc gtgagttacg gcaggatcaa
     6721 aaccttataa cagtcacact caggcaaaaa atccttcttg aatatccttt atgcatcgct
     6781 agagcaagga ttgaaagcgc aaagtatata tacaaccttt tttcccctcg tccttggatc
     6841 cgatcacgca gtcgatgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     6901 atcaacttga aaaagtggca ccgagtcggt gcttttttcc accactcttc acgcctaaag
     6961 ccaaagagac gcttattatg gccacatata cactgaagcg gtttggctct tggttattct
     7021 agaaaactct gtttttgaat taatcatagg aaaactgttt attctttttt gatatttcaa
     7081 cgtattacaa tgaatagtaa cttttgatta aaaaagagat gaagcaatac aagaaatgtt
     7141 gaaaagttta tgaaacatca cattagcgtg agttacggca ggatcaaaac cttataacag
     7201 tcacactcag gcaaaaaatc cttcttgaat atcctttatg catcgctaga gcaaggattg
     7261 aaagcgcaaa gtatatatac aacctttttt cccctcgtcc ttgcgacggc tcgttcatcg
     7321 ctggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     7381 agtggcaccg agtcggtgct ttttttgctg acggcggaag cgtcagcttc tagttctagt
     7441 gttcccacaa tgggcgcatg tgcttgtgtg tgaggcgtct ctctcttcgt ctctgttgcg
     7501 caaacgcata gactgcactg aaaaaatcga ttacctattt tttatgaatg aatatttgca
     7561 ctattactat tcaaaactat taagatagca atcacattca atagccaaat actataccac
     7621 ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc tgcatattta ggacggcatc
     7681 attatagaaa tgcttcttgc tgtgtacttt tctctcgtct ggcagctgtt tcgccgttat
     7741 tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta gtgaatgccc tactagaaga
     7801 tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa gtgcagatag cagtaaacgt
     7861 aagctaatat gaatattatt taactgtaat gttttaatat cgctggacat tactaataaa
     7921 cccactataa acacatgtac atatgtatgt tttggcatac aatgagtagt tggggaaaaa
     7981 atgtgtaaaa gcaccgtgac catcacagca taaagataac cagctgaagt atcgaatatg
     8041 agtaaccccc aaattgaatc acatgccgca actgatagga cccatggaag tacactcttc
     8101 atggcgatat acaagacaca cacaagcacg aacacccagt tgcggaggaa attctccgta
     8161 aatgaaaacc caatcggcga acaattcata cccatatatg gtaaaagttt tgaacgcgac
     8221 ttgagagcgg agagcattgc ggctgataag gttttagcgc taagcgggct ttataaaacg
     8281 ggctgcggga ccagttttca tatcactacc gtttgagttc ttgtgctgtg tggatactcc
     8341 tcccgacaca aagccgctcc atcagccagc agtcgtctaa tccagagaca ccaaaccgaa
     8401 agacttaatt tatatttatt taattaattt taataaaaca caccaaatgt aagtagcttt
     8461 ccccttccca acaacaaaac accatcgaac cactcccacc aagaaaaagc aataatcgag
     8521 aaaagccgcg gaaaatgtgt gatttttttt gtaaacaaaa cattttttta tgtgccagtg
     8581 ctgaaagtga tcaaaaaata ctagccacga gctaaagagt tattgtattg accaaaactc
     8641 caaaaatacc caagtttggc cctaaattgt caatcaaaat accaataggt cgaaagacat
     8701 caaaattaac aaaaccaggg tttcaaatac cataactcaa gaatcaggat tacaactgca
     8761 gatttcagga tatatacata caaattatac gaaattataa aaaccaaagc aattcaatag
     8821 ccccaactca aatgttagga tctaatatag tgtttaaagc caagctcgct gatgtgggcg
     8881 tgtcacgatt tcacccaaag atatgccaaa ttacgaattg caaatcaatt cgccaacact
     8941 ttcttttttt cccacgccct aaaacaccag atcatccata aatgtacata catacagtat
     9001 atgcatatta taatctataa aactagatca ggttcttgaa aatagtgacg taggcagccg
     9061 ttttggctta agcagaaatt tttgccggtt tttcaaagtt gtagttgcaa aaatggagaa
     9121 aaccttcgag cattcgttca tgtacacaca ctcacgcgca aaataacgag agagagtgta
     9181 tgtgtgtgtg agagagcgaa agccagacga cggtttgctt ttcgcctcga aacatgacca
     9241 tatatggtca caaaacttgg ccgccgcaat tcaacacacc agcgctctcc ttcgcaccca
     9301 tagcgaccat ggcgcggagc gagcgagatg gcgagagcga gcgacgccta tggcgacgtc
     9361 gacgcaggca gcgattgaaa aacgcagtta actggcattc aacattcacc agccactttc
     9421 agtcggttta ttccagtcat tcctttcaaa ccgtgcggtc gcttagctca gcctcgccac
     9481 ttgcgtttac agtagttttc acgccttgaa tttgttaaat cgaacaaaaa ggtaaagttt
     9541 aactagcttt gaaaagtttc gtggctctta attgttaaat tttctagagt gcgtttagtg
     9601 tttttttttt tattttgtaa tgttaatttc gggttccaat tcgagtttta ggcagccgcc
     9661 attttaaggg cgcatacaca caggcaactg tgctctcttt gcggctttct tttgcaccgg
     9721 cattcgttaa gtgtcgtcta gaagcttctc ccctcccttt tcggcatatt cgtattgtgg
     9781 ttttaatttt tcggggcggg gccttctatt ttgtaactgt tcttttaatt tcttattaca
     9841 attcgatcgc aagtgaaaat cagttttcaa tcggaaaagt atttttttat gaaatttttt
     9901 tttgtccaag attaaaattt tgtactaaaa aaacgtacat tgcattgagt gatttttaat
     9961 tgtacacgaa aaacaagtta gtttgttatg acaattgtat tttggtaaat cagcgcagtc
    10021 caaggaaacc acgcaaattc tcagtttttt tttttgccat ttctacatta ccaaataagg
    10081 taaccaaaaa ctaatgggaa atccgcattc tttccattgc agcttacaat ggtgagcaag
    10141 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    10201 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    10261 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    10321 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
    10381 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    10441 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    10501 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    10561 aactacttta gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
    10621 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
    10681 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    10741 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    10801 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg atgacggccg
    10861 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    10921 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    10981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tccattctag ttgtggtttg
    11101 tccaaactat aacttcgtat aatgtatgct atacgaagtt attactcgag gtcgacggta
    11161 tcgataagct tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
    11221 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
    11281 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
    11341 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
    11401 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
    11461 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
    11521 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
    11581 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
    11641 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
    11701 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
    11761 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
    11821 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
    11881 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
    11941 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    12001 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    12061 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    12121 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    12181 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12301 cgcaggaaag aacatgcgat gtagaacgcg agcaaattct tttccttcca tgacagcagc
    12361 agccacagtg ggaagcctaa cgtcagacgt gtttgacatg ccgagctggg cgggaaaatt
    12421 accgcgtgcg cattgttttc aagcaaatca caactcgctg caaacaaaac cgttgagaaa
    12481 ttgattgttt tataatttgt attttatttt atttgttata ataaactaaa aagacatact
    12541 ttttgcattt tttatacata aaaacataca tacagcatta taaaacacat ataagccctc
    12601 cctgtagagt cccgtgtcga aatcttccat ccaagttgca cagtacgacg gacgagtagg
    12661 ccgtgtgggt gcaaaatcca gcattttgct gtcttttgct cggagtactc tcggcgagtc
    12721 ggaggtttct gctgacgtgc ttagcgctaa attagccaat tgcttttgca agtgaaataa
    12781 ccagccgaat agtacttcaa aactcaggta agtgaactag ttttatagaa caaatgtttg
    12841 tttgttagaa gttagtgaag tgtttgtgaa aaaaatctct catttcggca aaactaacgt
    12901 aactgatttc aaattgaatt attgttttgt gatgttatat tatttcatcc agttgattag
    12961 tattttctta gttatgttca aaatacagtt aaattaaatt tcatttcatt tactcataaa
    13021 ataatctctt ggcttattta atttttctcg aatttgcttg tattgttcag tagcacgcgc
    13081 cattcgccct ttgttacatt ttgtacctgc tcccactaac acactggcag tgcgaaacaa
    13141 aagccttcgc acgcgttgct ggtattagag tgtgtgcgtg tgtgtgttga gcgctctgtc
    13201 aaaatcggct gttgccgccg gtaccgaaat tgcctgttcg cacgctgttc gtaaacattc
    13261 cgtggtgtgt atcgtgtgtt gtgcatattg cgcgcctccc ccccttatga tagcaaaagg
    13321 ttgccgtggt gtgtggcgca gttgagtttt tggattaatt ttctaaggaa atggcacgag
    13381 aagagcggtg gcagtgtgtt ggtttgctct gtcccttcct ttctgtgtga agtgttctta
    13441 cagcacagca cgtatccacc accgcacaca gagcaggcaa ggaagtggaa gtgaacaagt
    13501 gtgctgcgca tgcatgtgtg tggggggcat tttagctgag atcgtcgtta tttgagaagc
    13561 ggtatagggg ccagtcggtg tcgacgtacg gaagcgattt agttttaatc caagcgtatc
    13621 ccgtcgtgga gtggttgtgt ggctctgtgt gctctcatat cagttcctga gtgaggttag
    13681 tagaatcaca gtccttggcc tttttcgtta caagatatcc agaaggatgg cgttatttcc
    13741 acagcttacc atggtgctct tgtgtgctcg aatcaggggt gaaaatccgt tttgtgtttc
    13801 atgaaccgca gttggcactg gagcggattc ataagtcttc gatatgcaat agataagaga
    13861 gacgttgggg catagttggg aagctttccg agatgtggag tttccgagag gagaaatggt
    13921 gctttcgtgc acgttccggg acagcgggcc ccgcgaagag catctcgttg tcgttcatcc
    13981 ggcaataatt gatgcgaaaa gcgcgcgcgc cactggctta gcgcagtgta cacagtgata
    14041 ttcacctaca cacacagagg cacacgcctt cacacgcgcg cgtgcttcaa aggctacttc
    14101 ggtagcggtg tgtgaggtcg cttgcaatgg acaatgaaaa tttcgctgga aaataccatc
    14161 gtcgctttag gttgctatga gtgcgggtag agcggtggtc gtcgatagtg gtggtgtagt
    14221 gtgtgtgtgt gcaacggcaa ttatttttta taatatttcg accatctttc tttctctctc
    14281 tccacgtgct gctgctgctg cattgcatgt tccactattc ctctcggttt gtgcctgcgg
    14341 acgccattgc tagtcgaaag agagtcgccg ttagtcgcgc ttcgagcaac ggacacgttt
    14401 tttggttgaa accaacagct tttttcatct tcgggagaca cacagatctc gaatcgtaca
    14461 ttcccaaagg agaattgtca tcttccggtg aataaagaaa ggaaacaata tgggctctag
    14521 cctggacgac gagcacatcc tgagcgccct gctgcagagc gacgacgaac tggtgggcga
    14581 ggacagcgac agcgaggtca gcgaccacgt gtccgaggac gacgtgcagt ccgacaccga
    14641 ggaagccttc atcgacgagg tgcacgaagt gcagcctacc agcagcggct ccgagatcct
    14701 ggacgagcag aacgtgatcg agcagcctgg cagctccctg gccagcaaca gaatcctgac
    14761 cctgccccag agaaccatca gaggcaagaa caagcactgc tggtccacct ccaagagcac
    14821 caggcggagc agagtgtccg ccctgaacat cgtgcggagc cagaggggcc ccaccagaat
    14881 gtgcagaaac atctacgacc ccctgctgtg cttcaagctg ttcttcaccg acgagatcat
    14941 cagcgagatc gtgaagtgga ccaacgccga gatcagcctg aagaggcggg agagcatgac
    15001 cagcgccacc ttcagagaca ccaacgagga cgagatctac gccttcttcg gcatcctggt
    15061 gatgaccgcc gtgagaaagg acaaccacat gagcaccgac gacctgttcg acagatccct
    15121 gagcatggtg tacgtgtccg tgatgagcag agacagattc gacttcctga tcagatgcct
    15181 gagaatggac gacaagagca tcagacccac cctgcgggag aacgacgtgt tcacccccgt
    15241 gcggaagatc tgggacctgt tcatccacca gtgcatccag aactacaccc ctggcgccca
    15301 cctgaccatc gatgagcagc tgctgggctt cagaggcaga tgccccttca gagtgtacat
    15361 ccccaacaag cccagcaagt acggcatcaa gatcctgatg atgtgcgaca gcggcaccaa
    15421 gtacatgatc aacggcatgc cctacctggg cagaggcacc cagacaaacg gcgtgcccct
    15481 gggcgagtac tacgtgaaag aactgagcaa gcctgtgcat ggcagctgca ggaacatcac
    15541 ctgcgacaac tggttcacca gcatccccct ggccaagaac ctgctgcagg aaccctacaa
    15601 gctgaccatc gtgggcaccg tgcggagcaa caagcgggag atcccagagg tgctgaagaa
    15661 cagcagatcc agacctgtgg gaacaagcat gttctgcttc gacggccccc tgaccctggt
    15721 gtcctacaag cccaagcccg ccaagatggt gtacctgctg tccagctgcg acgaggacgc
    15781 cagcatcaac gagagcaccg gcaagcccca gatggtgatg tactacaacc agaccaaggg
    15841 cggcgtggac accctggacc agatgtgcag cgtgatgacc tgcagcagaa agaccaacag
    15901 atggcccatg gccctgctgt acggcatgat caatatcgcc tgcatcaaca gcttcatcat
    15961 ctacagccac aacgtgtcca gcaagggcga gaaggtgcag agccggaaga aattcatgcg
    16021 gaacctgtac atgagcctga cctccagctt catgagaaag agactggaag cccccaccct
    16081 gaagagatac ctgcgggaca acatcagcaa catcctgccc aaggaagtgc caggaacaag
    16141 cgacgacagc accgaggaac ccgtgatgaa gaagaggacc tactgcacct actgtcccag
    16201 caagatcaga agaaaggcca acgccagctg caagaaatgc aaaaaagtga tctgccggga
    16261 gcacaacatc gacatgtgcc agagctgttt ctgacatgtg agcaaaaggc cagcaaaagg
    16321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    16381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    16441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    16501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    16561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    16621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    16681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    16741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    16801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    16861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    16921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    16981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    17041 cctagatccc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
    17101 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
    17161 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
    17221 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
    17281 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
    17341 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
    17401 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
    17461 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
    17521 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
    17581 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
    17641 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
    17701 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
    17761 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
    17821 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
    17881 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
    17941 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
    18001 tgtaacactg gccatttccc cgaaaagtgc cac
//
