LOCUS       sequence-487722-        2431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Temperature-Inducible promoter, not including 
            ribosome binding site. Lacks repressor, cI857..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_PR
FEATURES             Location/Qualifiers
     source          1..2431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(136..154)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(137..154)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(301..889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(381..400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1004..1106
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1107..1766
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1173..1192)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1879..2334
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1966..1985)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2176..2197
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2353..2370
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2354..2370
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2377..2396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2377..2395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgttt gtgcaaccat tatcaccgcc agaggtaaaa tagtcaacac gcacggtgtt
       61 agatatttat cccttgcggt gatagattta acgtactccc gagaccactc atcgcccaat
      121 cactagtgaa ttcgttctat agtgtcacct aaataccata gagccgttgg tcatagctgt
      181 ttcctgtcca ggcatcatga cacggttatc cacagaatca ggggataacg caggaaagaa
      241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      961 catgagatta tcaaaaagga tcttcaccta gatccttttg tgatgatcgg cacgtaagag
     1021 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     1081 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1141 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1201 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1261 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1321 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1381 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1441 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1501 cctatttccc taaagggttt attgagaata tgtttttcgt gtcagccaat ccctgggtga
     1561 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1621 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1681 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1741 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
     1801 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgatgataa
     1861 gctgtcaaac atgatgttac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1921 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1981 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2041 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2101 atggttcacg tagtgggcca gcgccctgat agacggtttt tcgccctttg acgttggagt
     2161 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2221 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2281 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca attttaacat
     2341 tgtagaaacg catgtaaaac gacggccagt gaattgtaat acgactcact atagggtgaa
     2401 ttccgcttag gaccttagat attgatctca a
//
