LOCUS       sequence-487849-        3223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Arabinose-dependent transcriptional activator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_CDS_araC
FEATURES             Location/Qualifiers
     source          1..3223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..887
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        complement(928..946)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(929..946)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(962..978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(962..978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1093..1681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1173..1192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1796..1898
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1899..2558
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1965..1984)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2671..3126
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2758..2777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2968..2989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3146..3162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3169..3188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3169..3187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgaat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg tttaacgccc
       61 atctggtggc gggtttaacg ccgattgagg ccaacggtta tctcgatttt tttatcgacc
      121 gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag ggggtggtga
      181 aaaatcaggg acgagaattt gtctgccgac cgggtgatat tttgctgttc ccgccaggag
      241 agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag tgggtttact
      301 ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt gccaatacgg
      361 gtttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt gggcaaatca
      421 ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat ctgcttgagc
      481 aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg atggataatc
      541 gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat tttgatatcg
      601 ccagcgtcgc acagcatgtt tgcttgtcgc cgtcgcgtct gtcacatctt ttccgccagc
      661 agttagggat tagcgtctta agctggcgcg aggaccaacg cattagtcag gcgaagctgc
      721 ttttgagcac tacccggatg cctatcgcca ccgtcggtcg caatgttggt tttgacgatc
      781 aactctattt ctcgcgagta tttaaaaaat gcaccggggc cagcccgagc gagtttcgtg
      841 ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcataagct tcgagaccac
      901 tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca tagagccgtt
      961 ggtcatagct gtttcctgtc caggcatcat gacacggtta tccacagaat caggggataa
     1021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgtgatgatc
     1801 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1861 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1921 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1981 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     2041 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     2101 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     2161 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     2221 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     2281 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtgtcagcca
     2341 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     2401 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     2461 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     2521 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     2581 gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     2641 attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca ttaagcgcgg
     2701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2881 ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt tttcgccctt
     2941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3121 caattttaac attgtagaaa cgcatgtaaa acgacggcca gtgaattgta atacgactca
     3181 ctatagggtg aattccgctt aggaccttag atattgatct caa
//
