LOCUS       sequence-487914-       12236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary Plasmid for plant transformation expressing RUBY from the 
            Arabidopsis thaliana polyubiquitin 10 gene promoter and the 
            terminator from the pea RBCS gene. Kanamycin plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12236)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pATUBR
FEATURES             Location/Qualifiers
     source          1..12236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..311)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(324..1118)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(435..454)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1045..1064
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1140..1319)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(1424..2065)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(2071..6021)
                     /label=RUBY
     CDS             complement(3574..3630)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4465..4521)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7600..7624
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7933..8562
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8991..10064
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10130..10324
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        10386..10490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10491..11285
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11456..12044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11945..11964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    12180..12204
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gcggacgttt ttaatgtact ggggttgaac actctgtgcc gaattcggat ccagcgtcga
       61 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
      121 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
      181 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
      241 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
      301 tttgaacgat ctgcttgaca agctcagaag aactcgtcaa gaaggcgata gaaggcgatg
      361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
      421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
      481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
      541 aagcaggcat cgccctgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc
      601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
      661 acaagaccgg cttccatccg agtacgtcct cgctcgatgc gatgtttcgc ttggtggtcg
      721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
      781 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
      841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
      901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac
      961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     1021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     1081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcattt gagagtgaat atgagactct
     1141 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1201 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1261 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     1321 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1381 ccttaattct ccgctctccg caagaattca agcttagcgt cgattgatgc atgttgtcaa
     1441 tcaattggca agtcataaaa tgcattaaaa aatattttca tactcaacta caaatccatg
     1501 agtataacta taattataaa gcaatgatta gaatctgaca aggattctgg aaaattacat
     1561 aaaggaaagt tcataaatgt ctaaaacaca agaggacata cttgtattca gtaacatttg
     1621 cagcttttct aggtctgaaa atatatttgt tgcctagtga ataagcataa tggtacaact
     1681 acaagtgttt tactcctcat attaacttcg gtcattagag gccacgattt gacacatttt
     1741 tactcaaaac aaaatgtttg catatctctt atattttcaa attcaacaca caacaaataa
     1801 gagaaaaaac aaataatatt aatttgagaa tgaacaaaag gaccatatca ttcattaact
     1861 cttctccatc catttccatt tcacagttcg atagcgaaaa ccgaataaaa aacacagtaa
     1921 attacaagca caacaaatgg tacaagaaaa acatgtttcc caatgccata atactcgaac
     1981 tcagtaggat tctggtgtgt gcgcaatgaa actgatgcat tgaacttgac gaacgttgtc
     2041 gaaaccgatg atacgaacga aagctcaagc tcactggagg cttggctcaa gtttggcctg
     2101 catgacggcg tcgaggaaat cgtcgatcgc cttcacggag gagcccttca gctctttttc
     2161 ctcgttcacg gcgtccttga gctttttgcc catttccacg gcgcgattct tcatctccca
     2221 gcctttggag ccctcgttgc gctcaaggat catctcgacg atgcgcttga ccttctcttt
     2281 ggagatctcg ccttcgaggc ctctagcaag ctcaaccgcg acgcccatct catcttcgag
     2341 gtacttgagg ttgtaggcct gctcggcagc aagtggccat ccaagcatcg gaacgccctc
     2401 gcgaagagac tcaaggatgc tgttccatcc gcagtgggtg agaaagccgc cgatggattc
     2461 atggttgagg atctcaagct gcgggccgag cttgtacacg agcttgccct gtttcttcac
     2521 cttcatgcgt tcctcaaagc cctctggcag ccactctggg cgcatctctt cgttgatgtc
     2581 gaagccgaac ggggctctga tcacccagag gaatggcttc tcggaggact caaggccagc
     2641 agcgagttcc atcatctgcg tcgggctgat cgtgttctgg gagccgaagg agatgtagag
     2701 gacggagtcc ggctctttga ggctgagcca ctgcacgaac tcggcgcctg ttgaattgtt
     2761 gtcggaggag gagtgctgca ctggggaagc aataagtggg ccgatgcccc agatcggcag
     2821 cttggtgtag ttgcggagga tggagaagcc gagtgtctcg atctcttcca cggagttgca
     2881 gagccagcca aaagacttca tggactggcg gagctgcggc tggaaatact tggaccaatc
     2941 gtcggagccg tcggcatatc tgaggaaccg atggagctgg gacctgcgga acttatggtt
     3001 ctctgggaag cctggcagcg ggaactcttg gtcgtcggag tagttctggt gcgggagatc
     3061 gttccagatg gacacgtagg cggatgtgcc ataagcgccg cctgttgtga acaccacgcc
     3121 tgtagagccc acatccttgg ccacctggtc aacccagccg aggaacacat catggatcac
     3181 gcagagtggt ggcctggctg ggctgaaatg cctggagatg taatcgcgga ggtgcggatc
     3241 gaggctgatg gtggaatgga agagggacac cacgagcggg agtgtgagct tatcggtgtt
     3301 ctcgatgccc ggtgggagtc cgtggttggt ggagttgaat ggcagctcga cgatcctgat
     3361 gccgctggag gagtaggaat ggtggtgaag gaggtgcctg aggaagccgg cattgagcgg
     3421 agttgtgagc agggtgatga tcacgtggga gcgcttgtag aggaacatgg cgagttcgag
     3481 aaatggcctg aggtggccct gcgccatgaa cgggatcatg aggatgtgct gtgtctcgcc
     3541 ctcgccgttg gtgttcatct tgatggcggt catggggcca ggattttcct caacatctcc
     3601 tgcttgcttg agcaggctaa agttggttgc tccggatccg gcggaggtga acttgtagga
     3661 gccgtggcaa agtgtgccat gatcccagga ggagtggatc agctcggcct tccacttttc
     3721 accagcagcg ccgagcacaa cgtggagcgg gtagaagtgc tctggaaatg gatgggcgag
     3781 cttccagttc ggggccttgc tctcgtagat gttcacttcc tcgaagcggc cgttgatgag
     3841 ggccttccta agccaagaat cgaaggcagc ggcccaaggg gcaacaccat cgaagtagtg
     3901 cggtgtctca tcgagtggat gtgtggcgct gccggagcca ataatgagca cgccatcatt
     3961 cttgagcggc gcgagggctc tgccgagatt gtagtggtag gtgccgtcga gatgtggctg
     4021 aactgagagc tggcacaccg ggatgtccgc ctctggatac atgagcatga gtggaaccca
     4081 ggcgccgtga tcaaggcccc tcttttggtc tgtctcggct gtctcgaacc cggacttctt
     4141 gaggatctct tccacctttc tcgcaagctc tggctcgcct ggagctgggt acttgaactg
     4201 gtacatggcg gctgggtagt cgtcgaagtc gtggatggtg tcgttgatgt gcacggcgtt
     4261 cacggttggc ttcactgtct cccagtggcc ggagatgatg aggatggcct tcggcttctt
     4321 ggagaaaatc ttctcgcgcc atgtctcgaa gaacggcctg agcggatgtg tatcctcgac
     4381 ggtgaggatc gggttgccgt gggtgatgaa gaaggactct ttgatcatct ggtcgttggc
     4441 gtcctcgccg ttcatcatct tcataggccc ggggttctct tctacatcac ctgcctgctt
     4501 aaggaggcta aaattggtag ctccgctacc gtagcgcgga atcgggatga gcttgagcgg
     4561 cttggtcttt tggagcgcaa tgccgaactt ctcgtccatg tcgaggtcct tcggggagat
     4621 gtcgccttcg agcttccagt tgaagaactg gaggagtgtg gcgagcatga gggtgagcat
     4681 cctgatggcg agattcatgc ctgggcaaat tctgcggcca gcgccaaatg gaaggaggcc
     4741 gaaatcgcgg cccttaacat cgatctcgca gccgatgaag cgctctgggc tgaaaatatc
     4801 ggcgttctgc caggcgtttg ggtccctgcc aatggcccag aggttcacga ggatctgggc
     4861 atccttcggc acgatgtagc cgtacagctc gacatcggtg tcggcctttc ttgggagcaa
     4921 gaacacggtc ggcggatgga ggcggagtgt ctctttgatg atcgcctgga ggtacggcag
     4981 gttgatgatg tcggactctt ggatctgctt gtccttgccg aggacctgct tgatttcctc
     5041 ttgggccttt tccatcatct ctggattgcg gatcagctcg gtcatcaccc actcgaatgt
     5101 ggaggatgtg gtatcggtgc cggcgtcgaa gatgtccacg aggaggtggt tgatctcgcc
     5161 catcgtcagc tcgttctgct tgaacagctg gaggagcaca tcgagcacgt cgtcggtggt
     5221 ggtggtagtt gtggtggagg aatctggggc gagtctctcg cagatgatgc cctggaacac
     5281 ggcgatcagc ttgtcgaagg agcaggcgag ccttcttcta atgccagatg ggtcaacgca
     5341 gccgagaatc gggaagtagt cggcgtagtt cggcttgcct atatcttcca tgatgttcca
     5401 gatcagctct ttgaactctt ggctggtatg ggacttgtgg tgcgccagct cgacgctgaa
     5461 gaacagcttg ctgaggaggt tgaggctggt cgtaaaggcg gccttgccaa tatccacggc
     5521 ctggcctttc tgggcgcact cttgaacgta ctcgtagagc tgctgcacct tggcgtgcct
     5581 gaatgtctgg caggcatcga gtctctgtgg ggagagcaga tgcacggcgg taatcttgcg
     5641 gaagttgcgc cacttcggag acaccgggag ccaagacatg gtgagcttgt ggtggtcgcc
     5701 ggctgtaaca ctattcggga tcgtgcggtt ggagagcggg tgatccttct tgaggaacat
     5761 ctctttggcc acgtcggcag aggacacaac gatggtggtc acagagccga gtctgaggga
     5821 aatgagtggg ccgtgaatct tggcgaggtt ggcgaaggac cgatgcggct tcttgcccac
     5881 ctcaaggatg ttgccgatga tcggaagcgg ctttggtcct ggcggaagca gcttggtggt
     5941 ctgctgggag aacagcagct tgatgaagtg gaagctgatg aaccagatcg cgaggatcat
     6001 ggcgagggtc gcatgatcca tcattaagat ctgcatctgt taatcagaaa aactcagatt
     6061 aatcgacaaa ttcgatcgca caaactagaa actaacacca gatctagata gaaatcacaa
     6121 atcgaagagt aattattcga caaaactcaa attatttgaa caaatcggat gatatctatg
     6181 aaaccctaat cgagaattaa gatgatatct aacgatcaaa cccagaaaat cgtctacgat
     6241 ctaagattaa cagaatctaa accaaagaac atatacgaaa ttgggatcga acgaaaacaa
     6301 aatcgaagat tttgagagaa taaggaacac agaaatttac cttgatcacg gtagagagaa
     6361 ttgagagaaa gtttttaaga ttttgagaaa ttgaaatctg aattgtgaag aagaagagct
     6421 ctttgggtat tgttttatag aagaagaaga agaaaagacg aggacgacta ggtcacgaga
     6481 aagctaaggc ggtgaagcaa tagctaataa taaaatgaca cgtgtattga gcgttgttta
     6541 cacgcaaagt tgtttttggc taattgcctt atttttaggt tgaggaaaag tatttgtgct
     6601 ttgagttgat aaacacgact cgtgtgtgcc ggctgcaacc actttgacgc cgtttattac
     6661 tggacttcgt ccgacaacca caatttcctt aacggtcgtc ataagatcca gccgttgaga
     6721 tttaacgatc gttacgattt atattttttt agcattatcg ttttattttt taaatatacg
     6781 gtggagctga aaattggcaa taattgaacc gtgggtccca ctgcattgaa gcgtatttcg
     6841 tattttctag aattcttcgt gctttatttc ttttcctttt tgtttttttt tgccatttat
     6901 ctaatgcaag tgggcttata aaatcagtga atttcttgga aaagtaactt ctttatcgta
     6961 taacatattg tgaaattatc catttctttt aattttttta gtgttattgg atatttttgt
     7021 atgattattg atttgcatag gataatgact tttgtatcaa gttggtgaac aagtctcgtt
     7081 aaaaaaggca agtggtttgg tgactcgatt tattcttgtt atttaattca tatatcaatg
     7141 gatcttattt ggggcctggt ccatatttaa cactcgtgtt cagtccaatg accaataata
     7201 ttttttcatt aataacaatg taacaagaat gatacacaaa acattctttg aataagttcg
     7261 ctatgaagaa gggaacttat ccggtcctag atcatcagtt catacaaacc tccatagagt
     7321 tcaacatctt aaacaagaat atcctgatct gaagaatgtg gaggctttag tcccttggat
     7381 acttgggagg ctgtggaaga acagaaacga gctggtgctt aaagggaggg aatttggaac
     7441 caatgaggta ttagtaagga cacaagaaga tgcagatgag tggattagaa ggaaagaggc
     7501 tcagaatgta aggaaagcac caaccacaac gacgtcctcc actagaggat gcacatgtga
     7561 ccgagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat attggcgggt
     7621 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     7681 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggagtcagc
     7741 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     7801 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     7861 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     7921 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     7981 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     8041 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     8101 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     8161 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     8221 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     8281 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     8341 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     8401 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     8461 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     8521 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     8581 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     8641 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     8701 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     8761 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     8821 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     8881 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     8941 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     9001 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     9061 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     9121 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     9181 cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     9241 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     9301 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     9361 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     9421 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     9481 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     9541 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     9601 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     9661 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     9721 agctagctga ttggatgtac cgcgagatta cagaaggcaa gaacccggac gtgctgacgg
     9781 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     9841 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     9901 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     9961 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    10021 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    10081 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct gtggatagca
    10141 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    10201 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    10261 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    10321 cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca tcaaggctcc
    10381 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
    10501 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
    10561 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
    10621 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
    10681 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
    10741 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
    10801 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
    10861 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
    10921 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
    10981 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
    11041 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
    11101 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
    11161 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
    11221 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
    11281 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12061 cctttttacg gttcctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg
    12121 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    12181 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacatt
//
