LOCUS       sequence-487922-        7548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_003, derived from human fusion protein ADIPOR2_FOXJ2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_003
FEATURES             Location/Qualifiers
     source          1..7548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1081..1791,1792..1794,1795..1797))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1123..1144)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1472..1491
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1731..1752
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1794..1803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1879..2082)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1908..1928)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2083..2386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2643..2687)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2872..3105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3465..3582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4074..4199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4246..4503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4505..4884
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4915..5019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5020..5088,5089..5880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5238..5257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6051..6639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6540..6559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6793..6810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6878..7012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6902..6921)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6956..6975
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7018..7213)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7032..7167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7272..7293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7308..7313,7314..7331,7332..7338)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7346..7362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7351..7373
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7370..7386
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7370..7386
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca atccgatggg aatgcaaaac cagtgttatt
      601 gataggggct tgaccctggg cttggggttg cgggggcggc ggctgctgag cagggtggct
      661 gtgtgggtgg tgttgatgcg gatgatagcc tccatgttga agcggagggg gatgcatcgc
      721 cataacaggg ggcgggccat atccttccgc acccgcttgc ttccctcctg gcggtgtaca
      781 accgtcggtg ctgggggtat gtaatggagg ggcacctccg acagctgaag ggccttgagc
      841 tggggcctgt tgctgctgag gttgtgattg ttgaggtggt ggttgagggg gttgctgctg
      901 ctgctgttgc tgttgtggtg gcggtggctg ctgctgctgg tacatataat agttattgga
      961 cggcggaatg tctcccagca ggctggagaa tccgtgctgt gatgaagaag atgacttttc
     1021 taacatggac ttatataacc gggaagcggc cgcacttccc ccggagcctc cgcttccgcc
     1081 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     1141 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc tcaggtagtg
     1201 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     1261 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     1321 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     1381 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     1441 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     1501 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     1561 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     1621 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     1681 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     1741 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     1801 ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt tcactaaacg
     1861 agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     1921 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     1981 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     2041 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     2101 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     2161 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2221 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2281 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2341 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2401 tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac gcgttcgaag
     2461 cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     2521 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     2581 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     2641 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     2701 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     2761 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     2821 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     2881 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     2941 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     3001 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     3061 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     3121 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3181 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctccttat
     3241 ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc cacacaatca
     3301 tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct tggcactact
     3361 tttatgtcac tattatcttg tattactact gccccttcac ctttccagag gagctttgct
     3421 ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     3481 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     3541 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     3601 atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat aattttcttt
     3661 aattctttat tcatagattc tactactcct tgactttggg gattgtaggg aatttccagg
     3721 tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc ttcctctatc
     3781 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     3841 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     3901 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt aactgcgaat
     3961 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     4021 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     4081 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     4141 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     4201 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     4261 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4321 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     4381 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa
     4441 gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc
     4501 ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     4561 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     4621 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     4681 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     4741 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     4801 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     4861 aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga
     4921 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4981 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5041 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5101 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5161 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5221 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5281 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5341 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5401 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5461 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5521 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5581 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5641 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5701 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5761 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5821 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5881 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5941 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6001 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6061 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6181 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6421 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6841 cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga taagatacat
     6901 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     6961 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttagcttttt
     7021 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     7081 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     7141 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     7201 ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7261 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7321 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7381 tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata agagaagagg
     7441 gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta aaacatgtaa
     7501 ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatat
//
