LOCUS       sequence-487950-        9945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_036, derived from human fusion protein COL3A1_CLU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_036
FEATURES             Location/Qualifiers
     source          1..9945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3478..4188,4189..4191,4192..4194))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3520..3541)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3869..3888
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4128..4149
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4191..4200
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4276..4479)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4305..4325)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4480..4783
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5040..5084)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5269..5502
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5862..5979
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6471..6596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6643..6900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6902..7281
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7312..7416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7417..7485,7486..8277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7635..7654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8448..9036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8937..8956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9190..9207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9275..9409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9299..9318)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9353..9372
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(9415..9610)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9429..9564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    9669..9690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9705..9710,9711..9728,9729..9735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9743..9759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9748..9770
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9767..9783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9767..9783
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gtgcggcaag ctgaacggca gatagtgatc
      601 gccacggtct ccctttccac ccggcgagcc gtcacgaccc ggcaaaccgt ccgaaccagg
      661 gttaccgtca cggcccggtt cgcccgccgt gcccgccaaa cccggcaggc cttgcgggcc
      721 cggcggaccg cgctctccgc tcaggccgtt cgcgcccggc ttcccagact ctcctttaac
      781 accctgtggt ccagggctac cacgcggacc cggcataccc ggaggtcccg ccaggccgcg
      841 agccccggtg atgccggcaa tacctaacgg ccccggcgcc cccggtggac cctgggcacc
      901 gggagatccc ttctcacccg gttgacccgc gtcgcctttc ggaccgctta cgcccggcga
      961 acccggggca ccggtgtttc ccgctggtcc tggggggcca tccttacccg ggctaccgct
     1021 tggtccaggt ggaccagggt tgccgttaga acccggcggt cccggcaaac cacgcgcccc
     1081 tgggaaacca gccgcacccg gaccacccgg ggaaccgcgc tcacccttca caccctgagg
     1141 acccggcggg cccgccgggc cggaaccacc cgggggaccg gcgacgcctg gagggccacc
     1201 ttcgcctttt tcaccgggag cgccacgctc accctttccg cctggctcac cattctgacc
     1261 cggagcaccc gggaagcccg caggtcccgg cgggcccgtc tctccgcgtt cgcccggtga
     1321 accgcgcggc ccggcgatac ccggtaaacc cggagcgcca ccttcgcctt tgtcccccgg
     1381 ctgacccgca ggccccggcg gaccgatggg accggtcgga cccctgggac cgtccttacc
     1441 cggcacgcca tcagcccccg gtccgcccgg ctcaccttta tcaccttttg ggcccgggct
     1501 gcccaaccca ccacgttcgc caggcatacc ttgcaagccc ggtgtaccag ctgcgccagg
     1561 cggacctggc ggacccgctg cgcccttgcc accttccgga cccggaggcc ccgcgccgcc
     1621 tctcaaaccc ggcgcaccag ctaagcccgg cggaccgcgc tctcccggcg cacccgcgtc
     1681 gcccttgccg cccggcgcac cgggagcacc tgcgtcgccc ttcgggcccg gctcgcccgg
     1741 cttcccattc tcgcccggag gtccaccggt acccggcaag ccctgcaagc cctgtggacc
     1801 cggcgggccg gtgtcacctt tgtcgccgcc aggaccagtt ggacctggtg gtccttgcgg
     1861 accggtttcg ccgttcttcc ccggcggacc ttgcggtccc ggaccacccg gaccgccgcg
     1921 ttcaccattt ttacccgggg cgccatcgtt acctttcggg cccgggaaac ccatcacgcc
     1981 aggctggcca cgcgggccgc tcggaccggg tgggccaggg cgaccggatt caccctggct
     2041 gcctggagga cccggctttc cgtccgaacc cgggccgccc ggcgatcccg gcattccacg
     2101 cataccggga ccacccggaa cgccgtcacg tcccggctcg cccgccgcac cacgtggacc
     2161 ggcgggcccc ggtgcaccgc gttctccagc aggacccttc tctcctggaa tcccgttggg
     2221 gccagccggg ccgcggaaac ccggtgcgcc acgttcaccc gccgctcccg gcagtccgtt
     2281 cgcgcccggc tcaccgggac tgccatcctt tccatcttcc cctttcgcgc ccggcacccc
     2341 cgggatgcct gcctcacccc gttcaccacg cggacccggc tcgcctttcg cgccgttctt
     2401 acccggctct cccgccccgc cccgcaggcc tggcgcacca ttcgcgcccg ccggaccggg
     2461 cgggccacga gcacccataa gccccggcgc gcccgggatg ccggcagggc ccatctcgcc
     2521 cttcccgccc gggctaccgt taatgcccgg cggtcccggt ggaccctgtg cgccggcatg
     2581 accttgcgga cctggctcgc cacgttgccc gggcgcacca ttagaacccg gcgaacccgc
     2641 gggaccaacc tcacctttag cccccggaga ccccgggaaa cccgcggtac ccggtggacc
     2701 tggcggacca ggctgaccgt cggaaccacg cgcgccatca ttgccgcgtg cacctgccgc
     2761 accagggagg ccaggacgtc cccgttcacc gggcgctcct ctcggcccca tagggcccgg
     2821 tgcgccgttc tcacccggaa ggccgttttc accttttaag cccggcgcgc cagtctcacc
     2881 tttttcaccg ttgcgcccat cgaaaccacg gtggcctttc atgcccggga aacccgggat
     2941 acctgccggg cctttaatcc cgggcgggcc gggcagtcct ctctcacccg gacgacctgg
     3001 acggccgctc tccccatcct tgccggcagg gccggaagga ccaatcgccc ccggcgggcc
     3061 tggcgggccg ctcgggcccg cttggccggg ctcacccgga ggaccctggt aacccggaga
     3121 gcccggggag cccgggtgac ctgaggtgcc aggcggacct ggtggccccg gcgggcccgc
     3181 cggacccgga taacctgcga gcccacccac cgcgacgccg cttttcacat cgtaagagtc
     3241 gtactgcggg ctatagttct gcgggcccgt cggacaggat tcgcatatac ccggcggacc
     3301 cggcgaccca gggctacccg gttggccagg aatacccgga tcgccattac gaccggggat
     3361 gcctggcggt cccgggtcgc ccttggggcc ctgcggaccc tgaccgttcg gcggacgcgt
     3421 tggcgcggtc ggcggccggg aagcggccgc acttcccccg gagcctccgc ttccgccctt
     3481 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     3541 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
     3601 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     3661 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     3721 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     3781 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     3841 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     3901 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     3961 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     4021 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     4081 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     4141 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc
     4201 gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcggttca ctaaacgagc
     4261 ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     4321 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     4381 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     4441 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     4501 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     4561 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     4621 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     4681 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     4741 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     4801 gctatgaact aatgaccccg taattgatta ctattaataa ctagcacgcg ttcgaagctt
     4861 gagctcggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     4921 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     4981 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     5041 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     5101 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     5161 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     5221 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     5281 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     5341 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     5401 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     5461 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     5521 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     5581 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tccttatggt
     5641 gttttactaa acttttccat gttctaatcc tcatcctgtc tacttgccac acaatcatca
     5701 cctgccatct gttttccata atccctaatg atctttgctt ttcttcttgg cactactttt
     5761 atgtcactat tatcttgtat tactactgcc ccttcacctt tccagaggag ctttgctggt
     5821 cctttccaaa gtggatttct gctgtccctg taataaaccc gaaaattttg aatttttgta
     5881 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     5941 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     6001 tgtactgctg tcttaagatg ttcagcctga tctcttacct gtcctataat tttctttaat
     6061 tctttattca tagattctac tactccttga ctttggggat tgtagggaat ttccaggtct
     6121 gaagatcctg ctgcttgctg tgcttttttc ttacttttgt tttgctcttc ctctatcttg
     6181 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     6241 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     6301 cccagtattt gtctacagcc ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt
     6361 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     6421 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     6481 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     6541 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     6601 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     6661 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     6721 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     6781 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     6841 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     6901 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     6961 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     7021 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     7081 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     7141 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     7201 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     7261 aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     7321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7381 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7441 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7501 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7561 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7621 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7681 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7741 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7801 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7861 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7921 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7981 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8041 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8101 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8161 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8221 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8281 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8341 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8581 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8821 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9001 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9061 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9241 ctccccgcgc gttggccgat tcattaatgc agcggatcca gacatgataa gatacattga
     9301 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     9361 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta gctttttgca
     9421 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     9481 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     9541 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     9601 attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9661 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9721 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9781 gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga gaagagggac
     9841 agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga
     9901 aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatat
//
