LOCUS       sequence-48805-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, 
            Zheng N, Major MB, Moon RT
  TITLE     Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 
            protein through competitive binding to KEAP1 protein.
  JOURNAL   J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3.
  PUBMED    22215675
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2012-02-24"; volume: "287(9)"; pages: "6539-50. Epub 
            2012 Jan 3"
COMMENT     Sequence Label: NC14 pGLUE FBXW11
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..220
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             239..259
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             266..292
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             266..292
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             293..370
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tctggctact gagaaccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc ttggtaccga gctcggatcg atttaattaa gccaccatgg acgagaagac
      121 caccggctgg cggggcggcc acgtggtgga gggcctggcc ggcgagctgg agcagctgcg
      181 ggcccggctg gagcaccacc cccagggcca gcgggagccc ggcggaagcc ccggtggcga
      241 gaacctgtac ttccagggcg caagctaccc ctacgacgtg cccgactacg ccaagcggcg
      301 gtggaagaag aacttcatcg ccgtgagcgc cgccaaccgg ttcaagaaga tcagcagcag
      361 cggcgccctg attgatgggg gatctggccc cggcggcggc gcgcctatgg agcccgactc
      421 ggtgattgag gacaagacca tcgagctcat gataagtaat ggaacatcat ctgtgatcgt
      481 ctccagaaag aggccatcag aaggaaacta tcaaaaagaa aaagacttgt gtattaaata
      541 ttttgaccag tggtctgaat cagatcaagt ggaatttgtg gaacatctta tttcacgaat
      601 gtgtcattat cagcatggac atattaactc ttacctgaag cccatgttgc agcgggactt
      661 tattaccgct ttaccagagc aaggcttaga tcacatagca gaaaacattc tttcgtacct
      721 ggatgccagg tctctgtgtg cagcagagct ggtatgtaaa gaatggcagc gagtgatctc
      781 agaaggaatg
//
