LOCUS       sequence-488104-        3069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, 
            not including ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_tetR_Ptet
FEATURES             Location/Qualifiers
     source          1..3069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(37..663)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     568..587
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        679..733
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    715..733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(774..792)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(775..792)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(808..824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(808..824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(939..1527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1019..1038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1642..1744
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1745..2404
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1811..1830)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2517..2972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2604..2623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2814..2835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2991..3008
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2992..3008
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3015..3034
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3015..3033
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gctcattttc gccagatatc gacgtcttaa gacccacttt cacatttaag
       61 ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc
      121 ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg
      181 tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt
      241 aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca
      301 taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa
      361 atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt
      421 acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc
      481 taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa
      541 tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc
      601 gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat
      661 cattaattcc taatttttgt tgacactcta tcgttgatag agttatttta ccactcccta
      721 tcagtgatag agacaaacga gaccactcat cgcccaatca ctagtgaatt cgttctatag
      781 tgtcacctaa ataccataga gccgttggtc atagctgttt cctgtccagg catcatgaca
      841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1621 ttcacctaga tccttttgtg atgatcggca cgtaagaggt tccaactttc accataatga
     1681 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     1741 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1801 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1861 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1921 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1981 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2041 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2101 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2161 tgagaatatg tttttcgtgt cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2221 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2281 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     2341 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2401 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag
     2461 tgataataag cggatgaatg gcagaaattc gatgataagc tgtcaaacat gatgttacgc
     2521 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2581 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2641 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2701 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccagc
     2761 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2821 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2881 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2941 gaattttaac aaaatattaa cgcttacaat tttaacattg tagaaacgca tgtaaaacga
     3001 cggccagtga attgtaatac gactcactat agggtgaatt ccgcttagga ccttagatat
     3061 tgatctcaa
//
