LOCUS       sequence-48811-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Kim J, Kato M, Beachy PA
  TITLE     Gli2 trafficking links Hedgehog-dependent activation of Smoothened 
            in the primary cilium to transcriptional activation in the nucleus.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 
            Dec 8.
  PUBMED    19996169
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-12-22"; volume: "106(51)"; pages: 
            "21666-71. Epub 2009 Dec 8"
COMMENT     Sequence Label: pCEFL3xHAmGli2
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..48
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             52..78
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aagcttgccg ccaccatggg atacccatac gatgttcctg actatgcggg ctatccctat
       61 gacgtcccgg actatgctgc tagctatcca tatgacgttc cagattacgc tagatccact
      121 agtgagactt ctgccccagc ccctgcactg gagaagaaag aagccaagag tggtctcttg
      181 gaggacagca gcttccccga cccagggaaa aaggcctgtc ctctggcggt ggccgcagct
      241 gtagccgccc acggagttcc tcagcagctc ctgccggctt tccacgcgcc tttgccgatt
      301 gacatgagac accaggaggg aaggtaccat tatgaccctc actctgtcca cagtgtacac
      361 gggcctccca ccctaagtgg cagccctgtc atctcagata tctccttgat acgactttct
      421 ccacaccctg ctggccctgg agagtcaccc ttcagcgccc accaccccta cgtgaacccc
      481 catatggagc actacctccg gtctgtgcac agcagcccca cactctcaat gatctctgcc
      541 gccaggggcc tcagccctgc tgatgtggcc cacgaacatc tgaaagagag gggactcttt
      601 agcctcgcag ccccaggcac caacccttca gactattacc accagatgac cctcatggca
      661 agccacccca ccccttatgg ggaccttcta atgcagagcg ggggtgctgc tagcgcaccc
      721 catctccatg actacctcaa ccctgtggat gcatcacgat tctctagtcc acgtgtgacc
      781 ccacgactga gccgcaagcg ggctctgtcc atctc
//
