LOCUS       sequence-48838-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, 
            Uney JB
  TITLE     Increased utility in the CNS of a powerful neuron-specific 
            tetracycline-regulatable adenoviral system developed using a 
            post-transcriptional enhancer.
  JOURNAL   J Gene Med. 2005 May;7(5):576-83.
  PUBMED    15580589
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene 
            Med."; date: "2005-05"; volume: "7(5)"; pages: "576-83"
COMMENT     Sequence Label: pXCX.CMV.tTA.WPRE
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     144..160
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     145..161
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(257..276)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca
      121 gattccatca cactggcggc cgctcgaggt cgacggtatc gataagcttg atatcgaatt
      181 catatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      241 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      301 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      361 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      421 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      481 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      541 ttatgccaac aaggtttttc actanagaat gcattatatg cactcagcgc tgtggggcat
      601 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      661 acacctacta ctgatagtat gccgcc
//
