LOCUS       sequence-48846-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid for Hb9 GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
COMMENT     Sequence Label: Lenti-Hb9-GFP
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    198..786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(251..271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(669..680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtaaagcgg ccaattcgga tccgtttaaa ctctagagga tccaccggtg gccgcctggg
       61 cccgttaacg tctcgagttt aaacctggaa aaacatggag caatcacaag tagcaataca
      121 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
      181 ccagtcacac ctcagacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
      241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
      601 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
      661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
      721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
      781 ccccgcctg
//
