LOCUS       sequence-48849-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDH2
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt cggcccagaa caatgctaaa gtagctgtgc taggggcctc tggaggcatc
      121 gggcagccac tttcacttct cctgaagaac agccccttgg tgagccgcct gaccctctat
      181 gatatcgcgc acacacccgg agtggccgca gatctgagcc acatcgagac caaagccgct
      241 gtgaaaggct acctcggacc tgaacagctg cctgactgcc tgaaaggttg tgatgtggta
      301 gttattccgg ctggagtccc cagaaagcca ggcatgaccc gggacgacct gttcaacacc
      361 aatgccacga ttgtggccac cctgaccgct gcctgtgccc agcactgccc ggaagccatg
      421 atctgcgtca ttgccaatcc ggttaattcc accatcccca tcacagcaga agttttcaag
      481 aagcatggag tgtacaaccc caacaaaatc ttcggcgtga cgaccctgga catcgtcaga
      541 gccaacacct ttgttgcaga gctgaagggt ttggatccag ctcgagtcaa cgtccctgtc
      601 attggtggcc atgctgggaa gaccatcatc cccctgatct ctcagtgcac ccccaaggtg
      661 gactttcccc aggaccagct gacagcactc actgggcgga tccaggaggc cggcacggag
      721 gtggtcaagg ctaaagccgg agcaggctct gccaccctct ccatggcgta tgccggcgcc
      781 cgctttgtct tctcccttgt ggatgcaatg aatggaaagg aaggtgttgt ggaatgttcc
      841 ttcgttaagt cacaggaaac ggaatgtacc tacttctcca caccgctgct gcttgggaaa
      901 aagggcatcg agaagaacct gggcatcggc aaagtctcct cttttgagga gaagatgatc
      961 tcggatgcca tccccgagct gaaggcctcc atcaagaagg gggaagattt cgtgaagacc
     1021 ctgaagtaac agtaaaggtg gatacggatc cgaa
//
