LOCUS       sequence-488519-        5398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid encoding the human alpha-SYN under the control 
            of the CMVie enhanced synapsin1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Oliveras-Salva M, Van der Perren A, Casadei N, Stroobants S, Nuber 
            S, D'Hooge R, Van den Haute C, Baekelandt V
  TITLE     rAAV2/7 vector-mediated overexpression of alpha-synuclein in mouse 
            substantia nigra induces protein aggregation and progressive 
            dose-dependent neurodegeneration.
  JOURNAL   Mol Neurodegener. 2013 Nov 25;8:44. doi: 10.1186/1750-1326-8-44.
  PUBMED    24267638
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2013-11-25"; volume: "8:44. doi"; pages: " 
            10.1186/1750-1326-8-44"
COMMENT     Sequence Label: pAAV-CMVenh synapsin-intron-aSYNUCLEIN WT
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1009..1141
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1124..1143
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(1661..1681)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2079..2090)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2157..2174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2163..2370
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2458..2587
                     /label=AAV2 ITR
     primer_bind     complement(2606..2623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2606..2622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2615..2637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2764..3219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2851..2870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3061..3082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3246..3350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3351..3419,3420..4211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3569..3588)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4382..4970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4871..4890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5258..5279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5294..5299,5300..5317,5318..5324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5332..5348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5337..5359
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5356..5372
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5356..5372
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctaga tagttattaa tagtaatcaa ttacggggtc
      241 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      301 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      361 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      421 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      481 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      541 gtacatctac gtattagtca tcgctattac catggcacct gcagagggcc ctgcgtatga
      601 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
      661 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      721 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      781 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      841 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
      901 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      961 gaccatctgc gctgcggccg cactagttgg tcgtgaggca ctgggcaggt aagtatcaag
     1021 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     1081 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     1141 ggtgtccagg cggccgctct agaggatcta ccatggatgt attcatgaaa ggactttcaa
     1201 aggccaagga gggagttgtg gctgctgctg agaaaaccaa acagggtgtg gcagaagcag
     1261 caggaaagac aaaagagggt gttctctatg taggctccaa aaccaaggag ggagtggtgc
     1321 atggtgtggc aacagtggct gagaagacca aagagcaagt gacaaatgtt ggaggagcag
     1381 tggtgacggg tgtgacagca gtagcccaga agacagtgga gggagcaggg agcattgcag
     1441 cagccactgg ctttgtcaaa aaggaccagt tgggcaagaa tgaagaagga gccccacagg
     1501 aaggaattct ggaagatatg cctgtggatc ctgacaatga ggcttatgaa atgccttctg
     1561 aggaagggta tcaagactac gaacctgaag cctaacacgc gtgatccaat caacctctgg
     1621 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1681 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1741 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1801 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1861 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     1921 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     1981 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     2041 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2101 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcga gatctgcctc
     2161 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2221 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2281 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2341 ttgggaagac aatagcaggc atgctgggga ctcgagttaa gggcgaattc ccgataagga
     2401 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     2461 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2521 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2581 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2641 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2701 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2761 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2941 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3181 ttaacgcgaa ttttaacaaa atattaacgt ttataatttc aggtggcatc tttcggggaa
     3241 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3301 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3361 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3421 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3481 acatcgaact ggatctcaat agtggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3541 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3601 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3661 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3721 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3781 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3841 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagtaa
     3901 tggtaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3961 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4021 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4081 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4141 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4201 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4981 acgcggcctt tttacggttc ctggcctttt gctgcggttt tgctcacatg ttctttcctg
     5041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5161 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5221 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5281 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5341 gataacaatt tcacacagga aacagctatg accatgatta cgccagattt aattaagg
//
