LOCUS       sequence-488923-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet- or Dox-inducible expression of QtEnC fused to sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  TITLE     A Genetically Encoded Device for Transcriptome Storage in Mammalian 
            Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKC078_pLenti_TetON_QtEnC_sfGFP
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3620..3638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3692..3710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3728..3746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3764..3782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3899..3923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4930..5643
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4969..4990)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5577..5598
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5681..5700)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5746..5801
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5800..5819)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        6005..6216
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6229..6497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      6532..6541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6538..7248
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             7255..7308
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7309..8055
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8068..8656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8121..8141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8539..8550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8731..8964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9083..9101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9169..9273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9274..9342,9343..10134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9492..9511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag accggtggcc
     3601 tccacggcca ctagattact ccctatcagt gatagagaac gtatgaagag tttactccct
     3661 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3721 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3781 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     3841 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     3901 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     3961 actttccgta ccacttccta ccctcgtaaa gaagttccta ttccgaagtt cctattcttc
     4021 aaaaggtata ggaacttcgc tcgagccgcc accatgaata agtctcagct ctaccccgat
     4081 agcccgctca cggaccagga ttttaatcaa ctggatcaaa ccgtaatcga agctgcacga
     4141 cgccaacttg ttggaaggag atttatcgag ctgtatggtc ctttgggacg aggaatgcag
     4201 tctgtgttta atgacatatt catggagtcc catgaggcaa agatggattt ccaaggaagc
     4261 ttcgatacag aagtagagag ttcccggcgg gtcaactaca ccattcctat gctttacaaa
     4321 gatttcgttc tttactggag agatctcgag cagtccaaag cgctggatat accgatcgac
     4381 tttagcgtgg cagccaacgc agctcgagat gttgcttttc ttgaggatca gatgatcttt
     4441 cacggaagta aggagttcga tattccgggg ttgatgaatg tgaaagggag gcttacgcac
     4501 ctcataggta attggtacga aagtggtaac gcattccaag atatagttga ggcgcggaac
     4561 aaactcttgg agatgaacca taatggacct tatgctcttg tgctgtctcc agagctttac
     4621 tccttgttgc atagggtcca caaagacacc aacgtcctcg aaatcgagca cgttcgggag
     4681 ctcatcactg ctggtgtctt ccaatcccca gtacttaaag gcaaaagcgg ggtgatagtt
     4741 aatacgggaa ggaataacct tgaccttgcc atcagtgaag attttgagac ggcttatctt
     4801 ggcgaagaag ggatgaatca cccctttaga gtttacgaaa ctgtggtcct tcggattaaa
     4861 cggcctgcgg ctatctgcac gctcatcgat cctgaggagg gcagcggtag tgggtcggga
     4921 tcaggctcca gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4981 gacggcgacg taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc
     5041 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5101 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5161 aagcgccacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5221 agcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5281 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5341 cacaagctgg agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag
     5401 aacggcatca aggccaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
     5461 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5521 cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg
     5581 gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga gctgtacaag
     5641 taaggatcca ccggatctag ataactgagc ggccgcctaa tctttttccc tctgccaaaa
     5701 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     5761 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     5821 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     5881 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     5941 cccctgctgt ccattcctta catatgacta gtaaggatct gcgatcgctc cggtgcccgt
     6001 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6061 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6121 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6181 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6241 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6301 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6361 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6421 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6481 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacac ctgcagccaa cgccaccatg
     6541 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
     6601 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     6661 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     6721 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     6781 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     6841 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     6901 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     6961 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     7021 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     7081 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     7141 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     7201 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgc tagcgagggc
     7261 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat gtctagactg
     7321 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc
     7381 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     7441 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     7501 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     7561 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     7621 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     7681 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     7741 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     7801 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct
     7861 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     7921 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     7981 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     8041 atgctccccg ggtaagtcga cgatcctaat caacctctgg attacaaaat ttgtgaaaga
     8101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8461 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8641 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     8701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8761 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8821 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8881 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8941 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9001 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     9061 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9121 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9241 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9421 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9481 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9781 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
