LOCUS       sequence-48898-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHGDH
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg caaatctgcg gaaagtgctc atcagtgaca gcctggaccc ttgctgccgg
      121 aagatcttgc aagatggagg gctgcaggtg gtggaaaagc agaaccttag caaagaggag
      181 ctgatagcgg agctgcagga ctgtgaaggc cttattgttc gctctgccac caaggtgacc
      241 gctgatgtca tcaacgcagc tgagaaactc caggtggtgg gcagggctgg cacaggtgtg
      301 gacaatgtgg atctggaggc cgcaacaagg aagggcatct tggttatgaa cacccccaat
      361 gggaacagcc tcagtgccgc agaactcact tgtggaatga tcatgtgcct ggccaggcag
      421 attccccagg cgacggcttc gatgaaggac ggcaaatggg agcggaagaa gttcatggga
      481 acagagctga atggaaagac cctgggaatt cttggcctgg gcaggattgg gagagaggta
      541 gctacccgga tgcagtcctt tgggatgaag actatagggt atgaccccat catttcccca
      601 gaggtctcgg cctcctttgg tgttcagcag ctgcccctgg aggagatctg gcctctctgt
      661 gatttcatca ctgtgcacac tcctctcctg ccctccacga caggcttgct gaatgacaac
      721 acctttgccc agtgcaagaa gggggtgcgt gtggtgaact gtgcccgtgg agggatcgtg
      781 gacgaaggcg ccctgctccg ggccctgcag tctggccagt gtgccggggc tgcactggac
      841 gtgtttacgg aagagccgcc acgggaccgg gccttggtgg accatgagaa tgtcatcagc
      901 tgtccccacc tgggtgccag caccaaggag gctcagagcc gctgtgggga ggaaattgct
      961 gttcagttcg tggacatggt gaaggggaaa tctctcacgg gggtttgaca gtaaaggtgg
     1021 atacggatcc gaa
//
