LOCUS       sequence-489231-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with S75P mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-S75P
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(520..540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(938..949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
       61 gactctagag gatcgaattc atgtcggcta ccgctgccac cgtcccgcct gccgccccgg
      121 ccggcgaggg tggcccccct gcacctcctc caaatcttac cagtaacagg agactgcagc
      181 agacccaggc ccaggtggat gaggtggtgg acatcatgag ggtgaatgtg gacaaggtcc
      241 tggagcgaga ccagaagcta tcggaactgg atgatcgcgc agatgccctc caggcagggg
      301 ccccccagtt tgaaacaagt gcagccaagc tcaagcgcaa atactggtgg aaaaacctca
      361 agatgatgat catcttggga gtgatttgcg ccatcatcct catcatcatc atcgtttact
      421 tcagcactta aggatccgtt aacaattcga tatcaagctt atcgataatc aacctctgga
      481 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
      541 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
      601 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
      661 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
      721 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
      781 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
      841 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
      901 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
      961 tccttcccgc gg
//
