LOCUS       sequence-48927-e        1212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1212)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FES
FEATURES             Location/Qualifiers
     source          1..1212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga ttccggaggt gcagaagccc ctgcatgagc agctgtggta ccacggggcc
      121 atcccgaggg cagaggtggc tgagctgctg gtgcactctg gggacttcct ggtgcgggag
      181 agccagggca agcaggagta cgtgctgtcg gtgctgtggg atggtctgcc ccggcacttc
      241 atcatccagt ccttggataa cctgtaccga ctggaagggg aaggctttcc tagcattcct
      301 ttgctcatcg accacctact gagcacccag cagcccctca ccaagaagag tggtgttgtc
      361 ctgcacaggg ctgtgcccaa ggacaagtgg gtgctgaacc atgaggacct ggtgttgggt
      421 gagcagattg gacgggggaa ctttggcgaa gtgttcagcg gacgcctgcg agccgacaac
      481 accctggtgg cggtgaagtc ttgtcgagag acgctcccac ctgacctcaa ggccaagttt
      541 ctacaggaag cgaggatcct gaagcagtac agccacccca acatcgtgcg tctcattggt
      601 gtctgcaccc agaagcagcc catctacatc gtcatggagc ttgtgcaggg gggcgacttc
      661 ctgaccttcc tccgcacgga gggggcccgc ctgcgggtga agactctgct gcagatggtg
      721 ggggatgcag ctgctggcat ggagtacctg gagagcaagt gctgcatcca ccgggacctg
      781 gctgctcgga actgcctggt gacagagaag aatgtcctga agatcagtga ctttgggatg
      841 tcccgagagg aagccgatgg ggtctatgca gcctcagggg gcctcagaca agtccccgtg
      901 aagtggaccg cacctgaggc ccttaactac ggccgctact cctccgaaag cgacgtgtgg
      961 agctttggca tcttgctctg ggagaccttc agcctggggg cctcccccta tcccaacctc
     1021 agcaatcagc agacacggga gtttgtggag aaggggggcc gtctgccctg cccagagctg
     1081 tgtcctgatg ccgtgttcag gctcatggag cagtgctggg cctatgagcc tgggcagcgg
     1141 cccagcttca gcaccatcta ccaggagctg cagagcatcc gaaagcggca tcggtgacag
     1201 taaaggtgga ta
//
