LOCUS       sequence-48953-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TWF2
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gcatccacgc cacggaagag ctgaaggaat tctttgccaa ggcacgggct
      121 ggctctgtgc ggctcatcaa ggttgtgatt gaggacgagc agctcgtgct gggtgcctcg
      181 caggagccag taggccgctg ggatcaggac tatgacaggg ccgtgctgcc actgctggac
      241 gcccagcagc cctgctacct gctctaccgc ctcgactcac agaatgctca gggcttcgaa
      301 tggctcttcc tcgcctggtc gcctgataac tcccccgtgc ggctgaagat gctgtacgcg
      361 gccacgcggg ccacagtgaa aaaggagttt ggaggtggcc acatcaagga tgagctcttc
      421 gggactgtga aggatgacct ctcttttgct gggtaccaga aacactgaca gtaaaggtgg
      481 ata
//
