LOCUS       sequence-489644-        2006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BsaI Golden Gate assembly plasmid encoding the EFS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone 
            CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
  TITLE     High-resolution profiling reveals coupled transcriptional and 
            translational regulation of transgenes.
  JOURNAL   Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
  PUBMED    39651241
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528"
COMMENT     Sequence Label: pKG1179_pPV1-EFS
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        662..873
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        919..1023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1024..1092,1093..1884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALITFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGXRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1242..1261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt ccmgaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg ggtctcatac
      661 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      721 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      781 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      841 ctttttcgca acgggtttgc cgccagaaca cagtaatgag agacctagac gtcaggtggc
      901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttataaat acattcaaat
      961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1021 agtatgagta ttcaacattt ccgtgtcgcc cttattacct tttttgcggc attttgcctt
     1081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1261 tcccgtrttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1741 tmtcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1981 atgaccaaaa tcccttaacg tgagtt
//
