LOCUS       sequence-48968-e        1224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1224)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ADH4
FEATURES             Location/Qualifiers
     source          1..1224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gcaccaaggg caaagttatt aaatgcaaag cagccatcgc ctgggaagca
      121 ggcaagcccc tttgcattga agaggttgaa gtagctcccc ccaaggctca tgaagttcgc
      181 attcagatca ttgctacctc cctgtgccat actgatgcca ctgttatcga ttctaaattt
      241 gagggcctag ctttcccagt gatcgttggc catgaggctg caggtattgt ggaaagtatt
      301 gggccaggag tgaccaacgt caaaccaggt gacaaagtaa ttccacttta tgcacctcta
      361 tgtagaaaat gcaagttttg tctgagtcca ctcacaaatt tgtgtgggaa aatcagtaat
      421 ctcaaaagtc ctgctagtga tcaacaacta atggaagaca aaaccagcag gtttacctgc
      481 aaaggaaaac cagtttacca tttctttgga accagtacat tctctcagta cactgtggtg
      541 tcagatatca atcttgccaa aatagatgat gatgcaaatt tagagagagt ttgtctgctt
      601 ggatgtgggt tttcaactgg ctatggggct gcaatcaaca atgccaaggt cacccctggt
      661 tcgacttgtg ctgtctttgg cctaggaggt gtgggtcttt ctgctgtaat gggttgtaaa
      721 gcagcaggag cttccagaat cataggtatt gacatcaaca gtgagaagtt tgtgaaggct
      781 aaagccctgg gagccactga ctgcctcaat cctagagact tacataaacc tatccaggaa
      841 gttatcattg aattgaccaa gggaggtgtg gattttgccc ttgactgtgc aggtggatct
      901 gaaaccatga aagcagccct ggactgtaca accgcaggct ggggatcatg tactttcatt
      961 ggagtagctg ctggtagcaa aggattgact gtttttccag aggagctaat aatcggccgt
     1021 actataaatg gaacattctt tggtggttgg aaaagtgtag attctatccc aaagctggtc
     1081 actgactata agaataagaa attcaatctg gatgcactgg tgacccatac cctgcctttt
     1141 gacaaaatca gtgaggcatt tgacctaatg aaccaaggaa aaagcatccg aacaatcctc
     1201 atcttttgac agtaaaggtg gata
//
