LOCUS       sequence-48996-e         522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 522)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PRKCG
FEATURES             Location/Qualifiers
     source          1..522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc acaccgagcg ccgcgggcgc ctgcagctgg agatccgggc tcccacagca
      121 gatgagatcc acgtaactgt tggcgaggcc cgtaacctaa ttcctatgga ccccaacggt
      181 ctctctgatc cctatgtgaa actgaagctc atcccagacc ctcggaacct gacgaaacag
      241 aagacccgaa cggtgaaagc cacgctaaac cctgtgtgga atgagacctt tgtgttcaac
      301 ctgaagccag gggatgtgga gcgccggctc agcgtggagg tgtgggactg ggaccggacc
      361 tcccgcaacg acttcatggg ggccatgtcc tttggcgtct cggagctgct caaggcgccc
      421 gtggatggct ggtacaagtt actgaaccag gaggagggcg agtattacaa tgtgccggtg
      481 gccgatgctg acaactgaag taaaggtgga tacggatccg aa
//
