LOCUS       sequence-490164-       12802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L2 binary T-DNA backbone, suitable for plant transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Abate BA, Hahn F, Chirivi D, Betti C, Fornara F, Molloy MC, Creasey 
            Krainer KM
  TITLE     Developing a Molecular Toolkit to ENABLE all to apply 
            CRISPR/Cas9-based Gene Editing in planta
  JOURNAL   bioRxiv 2025.11.09.687425
REFERENCE   2  (bases 1 to 12802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.11.09.687425"
COMMENT     Sequence Label: pGMF3
FEATURES             Location/Qualifiers
     source          1..12802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6126..6135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8140..8164
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8473..9102
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9531..10604
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10670..10864
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        10926..11030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11031..11825
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11996..12584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12485..12504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    12720..12744
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccttgtct tccagtggta tggggtaccg cacggtctgc caatccgacg
       61 gaggtttatg aattttccac cttttccaca agctcaacta gtattaacga tgtggattta
      121 gcaaaaaaaa cctgtaaccc taaatgtaaa ataacgggta agcctgccaa ccatgttatg
      181 gcagattaag cgtctttttg aagggcaccg catctttcgc gttgccgtaa atgtatccgt
      241 ttagcaataa ggaaattctt aatgaataat ccgtcgttac tcaatcatgc ggtcgaaacg
      301 atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt tatttgatgc aaaaacccgg
      361 cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg acgatgttat tgacgatcag
      421 acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc ccgaacaacg tctgatgcaa
      481 cttgagatga aaacgcgcca ggcctatgca ggatcgcaga tgcacgaacc ggcgtttgcg
      541 gcttttcggg aagtggctat ggctcatgat atcgccccgg cttacgcgtt tgatcatctg
      601 gaaggcttcg ccatggatgt acgcgaagcg caatacagcc aactggatga tacgctgcgc
      661 tattgctatc acgttgcagg cgttgtcggc ttgatgatgg cgcaaatcat gggcgtgcgg
      721 gataacgcca cgctggaccg cgcctgtgac cttgggctgg catttcagtt gaccaatatt
      781 gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt atctgccggc aagctggctg
      841 gagcatgaag gtctgaacaa agagaattat gcggcacctg aaaaccgtca ggcgctgagc
      901 cgtatcgccc gtcgtttggt gcaggaagca gaaccttact atttgtctgc cacagccggc
      961 ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta cggcgaagca ggtttaccgg
     1021 aaaataggtg tcaaagttga acaggccggt cagcaagcct gggatcagcg gcagtcaacg
     1081 accacgcccg aaaaattaac gctgctgctg gccgcctctg gtcaggccct tacttcccgg
     1141 atgcgggctc atcctccccg ccctgcgcat ctctggcagc gcccgctcta ggcttactat
     1201 aaggatgttt gcaatgaaac caactacggt aattggtgca ggcttcggtg gcctggcact
     1261 ggcaattcgt ctacaagctg cggggatccc cgtcttactg cttgaacaac gtgataaacc
     1321 cggcggtcgg gcttatgtct acgaggatca ggggtttacc tttgatgcag gcccgacggt
     1381 tatcaccgat cccagtgcca ttgaagaact gtttgcactg gcaggaaaac agttaaaaga
     1441 gtatgtcgaa ctgctgccgg ttacgccgtt ttaccgcctg tgttgggagt cagggaaggt
     1501 ctttaattac gataacgatc aaacccggct cgaagcgcag attcagcagt ttaatccccg
     1561 cgatgtcgaa ggttatcgtc agtttctgga ctattcacgc gcggtgttta aagaaggcta
     1621 tctaaagctc ggtactgtcc cttttttatc gttcagagac atgcttcgcg ccgcacctca
     1681 actggcgaaa ctgcaggcat ggagaagcgt ttacagtaag gttgccagtt acatcgaaga
     1741 tgaacatctg cgccaggcgt tttctttcca ctcgctgttg gtgggcggca atcccttcgc
     1801 cacctcatcc atttatacgt tgatacacgc gctggagcgt gagtggggcg tctggtttcc
     1861 gcgtggcggc accggcgcat tagttcaggg gatgataaag ctgtttcagg atctgggtgg
     1921 cgaagtcgtg ttaaacgcca gagtcagcca tatggaaacg acaggaaaca agattgaagc
     1981 cgtgcattta gaggacggtc gcaggttcct gacgcaagcc gtcgcgtcaa atgcagatgt
     2041 ggttcatacc tatcgcgacc tgttaagcca gcaccctgcc gcggttaagc agtccaacaa
     2101 actgcagact aagcgcatga gtaactctct gtttgtgctc tattttggtt tgaatcacca
     2161 tcatgatcag ctcgcgcatc acacggtttg tttcggcccg cgttaccgcg agctgattga
     2221 cgaaattttt aatcatgatg gcctcgcaga ggacttctca ctttatctgc acgcgccctg
     2281 tgtcacggat tcgtcactgg cgcctgaagg ttgcggcagt tactatgtgt tggcgccggt
     2341 gccgcattta ggcaccgcga acctcgactg gacggttgag gggccaaaac tacgcgaccg
     2401 tatttttgcg taccttgagc agcattacat gcctggctta cggagtcagc tggtcacgca
     2461 ccggatgttt acgccgtttg attttcgcga ccagcttaat gcctatcatg gctcagcctt
     2521 ttctgtggag cccgttctta cccagagcgc ctggtttcgg ccgcataacc gcgataaaac
     2581 cattactaat ctctacctgg tcggcgcagg cacgcatccc ggcgcaggca ttcctggcgt
     2641 catcggctcg gcaaaagcga cagcaggttt gatgctggag gatctgatat gagcttttac
     2701 ccaggagaca gagaatgcaa ccgcattatg atctgattct cgtgggggct ggactcgcga
     2761 atggccttat cgccctgcga cttcagcagc agcaacctga tatgcgtatt ttgcttatcg
     2821 acgccgcacc ccaggcgggc gggaatcata cgtggtcatt tcaccacgat gatttgactg
     2881 agagccaaca tcgttggata gctccgctgg tggttcatca ctggcccgac tatcaggtac
     2941 gctttcccac acgccgtcgt aagctgaaca gcggctactt ttgtattact tctcagcgtt
     3001 tcgctgaggt tttacagcga cagtttggcc cgcacttgtg gatggatacc gcggtcgcag
     3061 aggttaatgc ggaatctgtt cggttgaaaa agggtcaggt tatcggtgcc cgcgcggtga
     3121 ttgacgggcg gggttatgcg gcaaattcag cactgagcgt gggcttccag gcgtttattg
     3181 gccaggaatg gcgattgagt cacccgcatg gtttatcatc tccgattatc atggatgcca
     3241 cggtcgatca gcaaaatggt tatcgcttcg tgtacagcct gccgctctcg ccgaccagat
     3301 tgttaattga agatacgcac tatattgata atgcgacatt agatcctgaa tgcgcgcggc
     3361 aaaatatttg cgactatgcc gcgcaacagg gttggcagct tcagacactg ctgcgagaag
     3421 aacagggcgc cttacccatt actctgtcgg gcaatgccga cgcattctgg cagcagcgcc
     3481 ccctggcctg tagtggatta cgtgccggtc tgttccatcc taccaccggc tattcactgc
     3541 cgctggcggt tgccgtggcc gaccgcctga gtgcacttga tgtctttacg tcggcctcaa
     3601 ttcaccatgc cattacgcat tttgcccgcg agcgctggca gcagcagggc tttttccgca
     3661 tgctgaatcg catgctgttt ttagccggac ccgccgattc acgctggcgg gttatgcagc
     3721 gtttttatgg tttacctgaa gatttaattg cccgttttta tgcgggaaaa ctcacgctga
     3781 ccgatcggct acgtattctg agcggcaagc cgcctgttcc ggtattagca gcattgcaag
     3841 ccattatgac gactcatcgt taagcttcag aggaggaatt aagtaatgag ttttgatatt
     3901 gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa
     3961 gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt
     4021 tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat
     4081 gtctacaaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat
     4141 aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac
     4201 ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc
     4261 tccattagtg ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc
     4321 accgtctgtg tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat
     4381 cacgcgggcg atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt
     4441 tccgaaaatg tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac
     4501 tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg
     4561 ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaggag
     4621 ctgggcttta actacatcgg cccggtggac ggtcatgatg tgctggggct tatcaccacg
     4681 ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt
     4741 cgtggttatg aaccggcaga aaaagacccg atcactttcc atgccgtgct aaatttgatc
     4801 cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca aaaatctttg
     4861 gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt actccggcga
     4921 tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgc tacttcgacg
     4981 tggcaattgc cgagcaacat gcggtgacct ttgctgcggg tctggcgatt ggtgggtaca
     5041 aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag gtgctgcatg
     5101 acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga ccgcgcgggc attgttggtg
     5161 ctgacggtca aacccatcag ggtgcttttg atctctctta cctgcgctgc ataccggaaa
     5221 tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat accggctatc
     5281 actataacga tggcccgtca gcggtgcgct acccgcgtgg caacgcggtc ggcgtggaac
     5341 tgacgccgct ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac
     5401 tggcgatcct taactttggt acgctgatgc cagaagcggc gaaagtcgcc gaatcgctga
     5461 acgccacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta attctggaaa
     5521 tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag
     5581 gcagcggcgt gaacgaagtg ctgatggccc atcgtaaacc agtacccgtg ctgaacattg
     5641 gcctgccgga cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc
     5701 tcgatgccgc tggtatggaa gccaaaatca aggcctggct ggcataagct ttgtgtgagg
     5761 agatgtcgaa tgagcgcaca tgccctgccc aaggcagatc tgaccgccac cagcctgatc
     5821 gtatcgggcg gcatcatcgc cgcttggctg gccctgcatg tgcatgcgct gtggtttctg
     5881 gacgcagcgg cgcatcccat cctggcgatc gcaaatttcc tggggctgac ctggctgtcg
     5941 gtcggattgt tcatcatcgc gcatgacgcg atgcacgggt cggtggtgcc ggggcgtccg
     6001 cgcgccaatg cggcgatggg ccagcttgtc ctgtggctgt atgccggatt ttcgtggcgc
     6061 aagatgatcg tcaagcacat ggcccatcac cgccatgccg gaaccgacga cgaccccgat
     6121 ttcgaccatg gcggcccggt ccgctggtac gcccgcttca tcggcaccta tttcggctgg
     6181 cgcgaggggc tgcttctgcc cgtcatcgtg acggtctatg cgctgatcct tggggatcgc
     6241 tggatgtacg tggtattctg gccgctgccg tcgatcctgg cgtcgatcca gctgttcgtg
     6301 ttcggcacct ggctgccgca ccgccccggc cacgacgcgt tcccggaccg ccacaatgcg
     6361 cggtcgtcgc ggatcagcga ccccgtgtcg ctgctgacct gttttcactt tggcggttat
     6421 catcacgaac accacctgca cccgacggtg ccgtggtggc gcctgcccag cacccgcacc
     6481 aagggcgaca ccgcataagc ttgagctgag gagattttga atgcaaacgg aacacgtcat
     6541 tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg cacacacggc
     6601 agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag gacaattatt
     6661 agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta actcggtttg
     6721 tgggcaccca caactgggag aaagcaacga ggacgcagtg atccgccgtt gccgttatga
     6781 gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct accgcgccac
     6841 cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac gcaccactag
     6901 tgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt tagcagatgt
     6961 attacacggt attgatgcaa cgccgtgggc gttcagtccg tggatggtga tgcaggcgac
     7021 aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataag ctttgcctca
     7081 ggatttttga aatgacggtc tgcgcaaaaa aacacgttca tctcactcgc gatgctgcgg
     7141 agcagttact ggctgatatt gatcgacgcc ttgatcagtt attgcccgtg gagggagaac
     7201 gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc accgggaaaa cgtattcgcc
     7261 ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc tgtcagccat gacggattac
     7321 tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc gctgatcctt gacgatatgc
     7381 cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac cattcattct cattacggag
     7441 agcatgtggc aatactggcg gcggttgcct tgctgagtaa agcctttggc gtaattgccg
     7501 atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt ttctgaactg tcaaacgcca
     7561 tcggcatgca aggattggtt cagggtcagt tcaaggatct gtctgaaggg gataagccgc
     7621 gcagcgctga agctattttg atgacgaatc actttaaaac cagcacgctg ttttgtgcct
     7681 cgatgcagat ggcctcgatt gttgcgaatg cctccagcga agcgcgtgat tgcctgcatc
     7741 gtttttcact tgatcttggt caggcatttc aactgctgga cgatttgacc gatggcatga
     7801 ccgacaccgg taaggatagc aatcaggacg ccggtaaatc gacgctggtc aatctgttag
     7861 gcccgagggc ggttgaagaa cgtctgagac aacatcttca gcttgccagt gagcatctct
     7921 ctgcggcctg ccaacacggg cacgccactc aacattttat tcaggcctgg tttgacaaaa
     7981 aactcgctgc cgtcagttaa gcttgcaaga attcaagctt ggagtacgtt aattagttgt
     8041 ctgttttaag ccccgtgctc tccgccgggg cttttttatt aaaaaccact caagtggaag
     8101 acaagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat attggcgggt
     8161 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     8221 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggagtcagc
     8281 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     8341 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     8401 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     8461 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     8521 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     8581 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     8641 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     8701 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     8761 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     8821 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     8881 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     8941 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     9001 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     9061 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     9121 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     9181 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     9241 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     9301 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     9361 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     9421 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     9481 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     9541 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     9601 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     9661 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     9721 cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     9781 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     9841 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     9901 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     9961 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
    10021 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
    10081 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
    10141 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
    10201 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
    10261 agctagctga ttggatgtac cgcgagatta cagaaggcaa gaacccggac gtgctgacgg
    10321 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
    10381 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    10441 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    10501 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    10561 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    10621 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct gtggatagca
    10681 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    10741 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    10801 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    10861 cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca tcaaggctcc
    10921 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
    11041 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
    11101 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
    11161 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
    11221 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
    11281 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
    11341 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
    11401 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
    11461 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
    11521 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
    11581 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
    11641 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
    11701 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
    11761 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
    11821 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11881 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11941 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12001 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12061 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12121 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12601 cctttttacg gttcctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg
    12661 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    12721 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    12781 acgtttttaa tgtactgggg tt
//
