LOCUS       sequence-490179-        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for activated ARIH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  TITLE     E2 Variants for Probing E3 Ubiquitin Ligase Activities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GST-tag ARIH1 binding E2 variant L3A1-1
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..37
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             506..550
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(614..636)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(781..799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        867..971
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctccaaaa tcggatgaaa acctgtattt tcagggatcc atgcgggcgg ccacaaggag
       61 gctgaggtgg gagcttgaag aaatccgcaa agcagggatg aaaaacttcc gtaacatcca
      121 ggttgatgaa gctaatttat tgacttggca agggcttatt gttcctgaca accctccata
      181 tgataaggga gccttcagaa tcgaaatcaa ctttccagca gagtacccat tcaaaccacc
      241 gaagatcaca tttaaaacaa agatctatca cccaaacatc gacgaaaagg ggcaggtctg
      301 tctgccagta attagtgccg aaaactggaa gccagcaacc aaaaccgacc aagtaatcca
      361 gtccctcata gcactggtga atgaccccca gcctgagcac ccgcttcggg ctgacctagc
      421 tgaagaatac tctaaggacc gtaaaaaatt cgcaaagaat gctgaagagt ttacaaagaa
      481 atatggggaa aagcgacctg tggacggtct taacgacatc ttcgaggctc aaaagattga
      541 atggcatgaa taagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg
      601 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
      661 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      721 gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg
      781 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      841 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      901 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      961 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1021 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaagatg ctgaagatca
     1081 gttgggtgcm cragtgggtt acwtcgaact ggatytcacm gcggtaagat ccttgaaagt
     1141 ttcgcccsaa gaacgttttc caakgatga
//
