LOCUS       sequence-490181-        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for RNF14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  TITLE     E2 Variants for Probing E3 Ubiquitin Ligase Activities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GST-tag RNF14 binding E2 variant L3R14-1
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(568..590)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(735..753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        821..925
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatggcggc cagcaggagg
       61 ctgatgaagg agcttgaaga aatccgcaaa gcagggatga aaaacttccg taacatccag
      121 gttgatgaag ctaatttatt gacttggcaa gggcttattg ttcctgacaa ccctccatat
      181 gataagggag ccttcagaat cgaaatcaac tttccagcag agtacccatt caaaccaccg
      241 aagatcacat ttaaaacaaa gatctatcac ccaaacatcg acgaaaaggg gcaggtctgt
      301 ctgccagtaa ttagtgccga aaagtggcag cgggagacct ggaccgacca agtaatccag
      361 tccctcatag cactggtgaa tgacccccag cctgagcacc cgcttcgggc tgacctagct
      421 gaagaatact ctaaggaccg taaaaaattc gcaaagaatg ctgaagagtt tacaaagaaa
      481 tatggggaaa agcgacctgt ggactaagcg gccgcatcgt gactgactga cgatctgcct
      541 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
      601 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      661 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc
      721 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      781 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1081 taagatcctt gagagtttcg ccccgaagaa cgttttccat gatgagcact tttaaagttc
     1141 tgctatgtgg cscggattat cccgggttga cgccgggcaa gagcaactcg gtcgc
//
